Seed hemicelluloses tailor mucilage properties and salt tolerance

Yang, Bo; Hofmann, Florian; Usadel, Björn; Voiniciuc, Cătălin

doi:10.1101/2020.08.03.234708

Cited by 6 publications

(9 citation statements)

References 36 publications

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“…Plant cell walls not only determine cell shape and provide structural support, but they also act as the front line of various environmental stresses (Doblin and Pettolino, 2010;Liu et al, 2021a;Tenhaken, 2014;Voxeur and H€ ofte, 2016). Plant cell wall polysaccharides exhibit a striking variety in composition in accordance with environmental conditions, and therefore, there is a close relationship between the variety of plant cell wall polysaccharide compositions and the response of plants to environmental stresses (Corrêa-Ferreira et al, 2019;Doblin et al, 2010;Houston et al, 2016;Lenk et al, 2019;Liu et al, 2021b;Yang et al, 2021;Zhang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…CSLD share high amino acid similarity with CESA and is involved in the biosynthesis of several types of glycans (Bernal et al, 2007;Galway et al, 2011;Li et al, 2017;Peng et al, 2019). CSLDs can regulate different types of cell wall polysaccharide synthesis with tissue and organ specificity, and they play important roles in plant growth and development (Favery et al, 2001;Gu et al, 2016;Hu et al, 2018;Kim et al, 2007;Li et al, 2009;Peng et al, 2019;Wang et al, 2001;Wang et al, 2011a;Yang et al, 2021;Yoshikawa et al, 2013). In Arabidopsis, AtCSLD1 and AtCSLD4 are involved in pollen tube growth (Wang et al, 2011a).…”

Section: Introductionmentioning

confidence: 99%

“…Cell wall polysaccharides also play important roles in plant responses to environmental stresses (Corrêa-Ferreira et al, 2019;Houston et al, 2016;Lenk et al, 2019;Liu et al, 2021b;Tenhaken, 2014;Yang et al, 2021). Salinity stress is one of the most widespread abiotic stresses and has a serious threat to global food security.…”

Section: Introductionmentioning

confidence: 99%

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Cellulose synthase‐like protein OsCSLD4 plays an important role in the response of rice to salt stress by mediating abscisic acid biosynthesis to regulate osmotic stress tolerance

Zhao

Wang

et al. 2021

Plant Biotechnology Journal

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Cell wall polysaccharide biosynthesis enzymes play important roles in plant growth, development and stress responses. The functions of cell wall polysaccharide synthesis enzymes in plant growth and development have been well studied. In contrast, their roles in plant responses to environmental stress are poorly understood. Previous studies have demonstrated that the rice cell wall cellulose synthase-like D4 protein (OsCSLD4) is involved in cell wall polysaccharide synthesis and is important for rice growth and development. This study demonstrated that the OsCSLD4 function-disrupted mutant nd1 was sensitive to salt stress, but insensitive to abscisic acid (ABA). The expression of some ABA synthesis and response genes was repressed in nd1 under both normal and salt stress conditions. Exogenous ABA can restore nd1-impaired salt stress tolerance. Moreover, overexpression of OsCSLD4 can enhance rice ABA synthesis gene expression, increase ABA content and improve rice salt tolerance, thus implying that OsCSLD4-regulated rice salt stress tolerance is mediated by ABA synthesis. Additionally, nd1 decreased rice tolerance to osmotic stress, but not ion toxic tolerance. The results from the transcriptome analysis showed that more osmotic stress-responsive genes were impaired in nd1 than salt stress-responsive genes, thus indicating that OsCSLD4 is involved in rice salt stress response through an ABAinduced osmotic response pathway. Intriguingly, the disruption of OsCSLD4 function decreased grain width and weight, while overexpression of OsCSLD4 increased grain width and weight. Taken together, this study demonstrates a novel plant salt stress adaptation mechanism by which crops can coordinate salt stress tolerance and yield.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellulose synthase‐like protein OsCSLD4 plays an important role in the response of rice to salt stress by mediating abscisic acid biosynthesis to regulate osmotic stress tolerance

Zhao

Wang

et al. 2021

Plant Biotechnology Journal

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“…In the developing konjac corm (Gille et al, 2011), AkCSLA3 produces glucomannan that already has promising health care applications such as in the treatment of life style diseases (Behera and Ray, 2016). While AtCSLA2 is most important for seed mucilage biosynthesis in Arabidopsis (Yu et al, 2014;Voiniciuc et al, 2015), novel links between seed HM structure and salt tolerance provide an indication that fine-tuning HM structure could also be relevant for engineering stress-resistant crops (Yang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Modular biosynthesis of plant hemicellulose and its impact on yeast cells

Robert

Waldhauer

Stritt

et al. 2021

Preprint

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Interest in the engineering of polysaccharide-based biomaterials has emerged in recent years. Despite impressive advances on bacterial cellulose, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes. Here, we optimized the cultivation of the yeast Pichia pastoris for the orthogonal production of plant polysaccharides, and enhanced heteromannan (HM) production by assembling modular cellulose synthase-like A (CSLA) enzymes. Chimeric proteins swapping the domains of a plant mannan synthase and a glucomannan synthase led, in three cases, to higher yields or improved growth compared to the parental CSLA enzymes. Prolonged expression of a glucomannan synthase from Amorphophallus konjac (AkCSLA3) was toxic to yeast cells, as demonstrated by reduced biomass accumulation and elevated uptake of dyes that are normally restricted to the extracellular matrix. However, no growth inhibition was observed for CSLA variants producing relatively pure mannan or a CSLC glucan synthase. The toxicity of AkCSLA3 was reduced by swapping its C-terminal region with that of a mannan synthase. HM production was further boosted by co-expressing chimeric CSLA proteins with the MANNAN-SYNTHESIS-RELATED1 (MSR1) putative glycosyltransferase. Interestingly, Pichia cells either increased or decreased in size depending on the CSLA variant expressed, and most of them remained viable even producing copious amounts of hemicellulose. Therefore, yeast modified with non-toxic plant polysaccharides could represent a modular chassis to produce and protect sensitive cargo such as therapeutic proteins.

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“…Germination assays were performed in 24-well culture plates as described previously (Yang et al, 2021). Around 35 seeds were hydrated in 500 µl of water or 150 mM CaCl 2 solution per well.…”

Section: Methodsmentioning

confidence: 99%

Microtubule-associated IQD9 guides cellulose synthase velocity to shape seed mucilage

Yang

Stamm

Bürstenbinder

et al. 2021

Preprint

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SummaryArabidopsis seeds release large capsules of mucilaginous polysaccharides, which are shaped by an intricate network of cellulosic microfibrils. Cellulose synthase complexes is guided by the microtubule cytoskeleton, but it is unclear which proteins mediate this process in the seed coat epidermis (SCE).Using reverse genetics, we identified IQ67 DOMAIN 9 (IQD9) and KINESIN LIGHT CHAIN-RELATED 1 (KLCR1) as two highly expressed genes during seed development and comprehensively characterized their roles for cell wall polysaccharide biosynthesis and cortical microtubule (MT) organization.Mutations in IQD9 as well as in KLCR1 lead to compact mucilage capsules with aberrant cellulose distribution, which can be rescued by transgene complementation. Double mutant analyses revealed that their closest paralogs (IQD10 and KLCR2, respectively) are not required for mucilage biosynthesis. IQD9 physically interacts with KLCR1 and localizes to cortical MTs to maintain their organization in SCE cells. Similar to the previously identified TONNEAU1 (TON1) RECRUITING MOTIF 4 (TRM4) protein, IQD9 is required to maintain the velocity of cellulose synthases.Our results demonstrate that IQD9, KLCR1 and TRM4 are MT-associated proteins that are required for seed mucilage architecture. This study provides the first direct evidence that members of the IQD, KLCR and TRM families have overlapping roles in guiding the distribution of cell wall polysaccharides. Therefore, SCE cells provide an attractive system to further decipher the complex genetic regulation of polarized cellulose deposition.

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Seed hemicelluloses tailor mucilage properties and salt tolerance

Cited by 6 publications

References 36 publications

Cellulose synthase‐like protein OsCSLD4 plays an important role in the response of rice to salt stress by mediating abscisic acid biosynthesis to regulate osmotic stress tolerance

Cellulose synthase‐like protein OsCSLD4 plays an important role in the response of rice to salt stress by mediating abscisic acid biosynthesis to regulate osmotic stress tolerance

Modular biosynthesis of plant hemicellulose and its impact on yeast cells

Microtubule-associated IQD9 guides cellulose synthase velocity to shape seed mucilage

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