Seed physiological performance of soybeans with altered saturated fatty acid contents

Abstract

Soybean (Glycine max (L.) Merr.) seeds with elevated or reduced percentages of palmitate and elevated percentages of stearate were compared with seeds of typical composition in tests for germination, seedling growth rate and leachate conductivity. In general, seeds with altered compositions did well in these physiological tests, but their vigour tended to be negatively correlated with the percentages of stearate and palmitate in various lipid classes.

Agronomic Effects of Mutations in Two Soybean Δ9–Stearoyl‐Acyl Carrier Protein‐Desaturases

“…Seed fatty acid profiles and total seed oil for each genotypic class were consistent with those previously reported (Ruddle et al, 2012)—the primary effect of both the SACPD‐B and SACPD‐C mutations on seed oil composition was an increase in stearic acid and a decrease in oleic acid. Unlike previous studies (Rahman et al, 1997; Wang et al, 2001b), the high seed stearic acid levels due to these two mutations did not significantly reduce germination. Seedling fatty acid composition was affected primarily by the SACPD‐B mutation, with the effect of nearly doubling seedling stearic acid content.…”

“…Least squares means of seed fatty acid (g kg -1 ), and seed total oil (g kg -1 ) of 50 lines from the F 2 -derived populations, LLL-05-01 (SACPD-C mutant: fas nc ) × TCJWB03-806-7-19 (SACPD-B mutant: fas2 nc ) and LLL-05-14 (SACPD-C mutant: fas nc ) × TCJWB03-806-7-19. Unlike previous studies (Rahman et al, 1997;Wang et al, 2001b), the high seed stearic acid levels due to these two mutations did not significantly reduce germination. Seedling fatty acid composition was affected primarily by the SACPD-B mutation, with the effect of nearly doubling seedling stearic acid content.…”

“…Another study noted poor germination in seeds with over 300 g kg -1 stearic acid (Rahman et al, 1997); however, in Brassica rapa L. and Brassica napus L. the poor germination associated with elevated stearic acid has been attributed to low total oil, and normal oil with elevated stearic acid was not associated with low germination (Knutzon et al, 1992). Wang et al (2001b) observed a negative correlation between lipid saturates and both germination and seedling growth rate. Primomo et al (2002) noted that RG6 and RG8, two elevated stearic acid mutants developed by induced mutagenesis with CH 3 SO 3 C 2 H 5 (ethyl methanesulfonate), yielded lower than the check cultivars while another elevated stearic acid mutant developed in the same manner, RG7, yielded similarly to the check cultivars.…”

Neutral and polar lipid phase transition of soybeans with various saturated fatty acid contents

“…However, we have observed that soybeans with elevated stearate percentages have unpredictable and abnormal field germination and low yield. Wang et al (2) showed that seed with altered saturated fatty acid contents generally did fairly well in viability and vigor tests conducted at various temperatures under controlled laboratory conditions, but in a number of instances, seed vigor was negatively correlated with saturate content, especially stearate content.…”

Analysis of Quantitative Trait Loci (QTLs) for Seed Size and Fatty Acid Composition Using Recombinant Inbred Lines in Soybean

“…The fatty acid composition of soybean oil completely determines its nutritional value [12], storage compatibility [25], industrial properties [21] and potential food applications [24,28]. Modification of fatty acid composition of soybean oil makes it more competitive in various segments of the food and industrial oil markets [9], and to make it more nutritional [12] has been an important objective of plant breeding and molecular genetics in recent years [29]. Altered fatty acid composition has been developed through traditional plant breeding [33] and application of chemical mutagens [6,10,31] that have extended the range of the five major fatty acid normally found in soybean oil unfortunate.…”