Seed Priming of Rice Varieties with Decoyinine Improve Their Resistance against the Brown Planthopper Nilaparvata lugens

Abstract

The brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae) is one of the most destructive rice pests in Asia. The microbial metabolite decoyinine (DCY) has been extracted from Streptomyces hygroscopicus. Recent studies have suggested that treating rice seeds with DCY could improve the growth and yield of rice. To further assess the effects of priming the seeds of different rice varieties with DCY on rice seedling resistance against BPH, an age-stage, two-sex life table and choice test were applied to investigate the individual fitness, population parameters, and preference behavior of BPHs in this study. The results indicated that feeding on rice seedlings grown from seeds primed with DCY significantly affected BPHs’ adult longevity, oviposition period, fecundity, as well as the net reproductive rate (R0), intrinsic rate of increase (rm), finite rate of increase (λ), doubling time (DT), and population size in terms of BPH. The honeydew excretions and the weights of BPHs fed on DCY-pretreated rice plants were remarkably lowered. The two-way ANOVA results also showed that there were significant differences in the biological parameters, honeydew excretions, and of BPH weights owing to DCY treatment, rice variety, and the interactions between DCY treatment and rice variety. Additionally, the feeding and oviposition preferences of BPHs for the pretreated rice plants were reduced. Our results imply that the priming of seeds with DCY can improve rice resistance against BPH, which could facilitate the utilization of seed priming as a new avenue for effective crop protection.

Development and Application of the Novel Plant Growth Regulator Guvermectin: A Perspective

“…Recently, a growing body of evidence has suggested that GV also fortifies plants against pest damage (Figure ). Priming rice seeds with GV has been found to enhance resistance to brown planthopper (BPH, Nilaparvata lugens) and small brown planthopper (SBPH, Laodelphax striatellus) by significantly reducing their fertility. − Physio-biochemical analyses indicate that when GV-treated rice was fed upon by BPH, there was an enhancement in the activities of plant defense-related enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione S transferase (GST), peroxidase (POD), and polyphenol oxidase (PPO) . Additionally, transcriptomic analyses showed that these feeding events precede the upregulation of 15 resistance-related WRKY genes.…”

Colonization of Piriformospora indica enhances rice resistance against the brown planthopper Nilaparvata lugens

“…BPH strain was reared on TN1 plants in a chamber (27 ± 1 °C, relative humidity of 70 ± 5%, and a photoperiod of 16 h:8 h (L:D)). 42 2.2 The cultivation and inoculation of P. indica P. indica strain was provided by Jiangsu Lixiahe District Institute of Agricultural Sciences. P. indica stored at −80 °C was inoculated on the plate medium of potato dextrose agar (PDA) and cultured at 28 °C for 7 days, and then inoculated into the potato dextrose broth (PDB) liquid medium at 28 °C for 7 days at 180 rpm on an incubator shaker.…”

“…After laying, rice seedlings were replaced every 3 days, and the numbers of laid eggs were counted until the female adult died. 42…”

“…51 A Parafilm bag was attached to the stems of a 30-day-old rice seedling and a BPH female adult of newly emerged was confined in a bag. 42 At 24 h after feeding, the amount of honeydew secreted by the female adults on the control seedlings and P. indica-colonized (2 × 10 4 cfu/mL) rice seedlings was measured, and each treatment had 25 replicates.…”