Seed propagation and re-introduction of the U.S. federally endangered Hawaiian endemic, Platanthera holochila (Hbd.) Krzl. (Orchidaceae)

Zettler, Lawrence W.; Wood, Erin M.; Johnson, Lynnaun J. A. N.; Kirk, Anna K.; Perlman, Steven P.

doi:10.14712/23361964.2015.51

Cited by 6 publications

(3 citation statements)

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“…Occasionally, plant growth regulators can be added. Symbiotic germination media usually contain agar and any other ingredient with a complex carbohydrate source of fungus nutrition such as oatmeal (Guimarães et al 2013;Jiang et al 2015), potato , and corn meal (Zettler et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Cyrtopodium paludicolum germination with two Tulasnella isolates

et al. 2017

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Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. Th e aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its effi ciency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. Th e experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. Th e mycobiont CH01 was successfully isolated from Cyrtopodium paludicolum and identifi ed as Tulasnella sp. Treatments with both fungi reached a higher germination percentage than under asymbiotic conditions, indicating no specifi city in the relationship between Cyrtopodium paludicolum and the fungi. Th e results presented have the potential to advance research into the propagation and conservation of C. paludicolum, a native of the Cerrado biome.

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Section: Introductionmentioning

confidence: 99%

Cyrtopodium paludicolum germination with two Tulasnella isolates

et al. 2017

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“…Nevertheless, samples may be processed 3–4 days after collection if necessary (Swarts and Dixon 2017 ), or longer in some cases. Zettler et al 2011 , for example, recovered orchid endophytes from root samples that were collected 1-week prior. Similarly, Richardson et al ( 1993 ) had success in isolating a diverse assemblage of Rhizoctonia -like fungi from epiphytes in Costa Rica up to 3 weeks after collection.…”

Section: Introductionmentioning

confidence: 99%

Techniques for the collection, transportation, and isolation of orchid endophytes from afar: a case study from Madagascar

et al. 2017

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BackgroundTropical orchids need more study with respect to their mycorrhizal associations. For researchers in distant countries who aspire to study these orchids augmenting their conservation, the great distances involved, coupled with limited funds, pose formidable challenges. These challenges are sometimes exacerbated by political unrest, delays in securing permits, unexpected hardships, and the risk that the biological samples collected (e.g., roots harboring mycorrhizal fungi) will not survive long-distance transport.ResultsWe describe a protocol for the collection and transport of root samples from Madagascar orchids to labs in the United Kingdom (Kew) and the United States (Illinois) where Rhizoctonia-like fungi were subsequently isolated. Three separate trips were made spanning 4 years (2012–2015), with emphasis on the collection of roots from epiphytic, lithophytic, and terrestrial orchids inhabiting the Itremo Massif of the Central Highlands. Collectively, the trips to Madagascar resulted in the isolation of all major groups of Rhizoctonia-like fungi (Ceratobasidium, Tulasnella, Sebacina) from all three orchid growth forms (terrestrials, epiphytes and lithophytes). Sampling of terrestrial and epiphytes during the rainy season (January) yielded best results.ConclusionsOur study demonstrates that peloton-forming fungi in root samples can retain viability up to 3 weeks after collection.

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“…The Hawaiian Islands are host to three native orchid species, which are also endemic to the islands, Anoectochilus sandvicensis (tribe Cranichideae), Liparis hawaiensis (tribe Malaxideae) and the critically endangered species Peristylus holochila (= Platanthera holochila tribe Orchideae) [27,28]. Owing to the rarity of P. holochila , I focused this study only on L. hawaiensis and A. sandvicensis .…”

Section: Introductionmentioning

confidence: 99%

The carbon and nitrogen ecophysiologies of two endemic tropical orchids mirrors those of their temperate relatives and the local environment

Hynson¹

2016

R. Soc. open sci.

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Orchids are one of the most widely distributed plant families. However, current research on the ecophysiology of terrestrial orchids is biased towards temperate species. Thus, it is currently unknown whether tropical terrestrial orchids belong to similar trophic guilds as their temperate relatives. To examine the ecophysiologies of two tropical terrestrial orchids, I analysed the carbon and nitrogen stable isotope compositions and nitrogen concentrations of the Hawaiian endemics Anoectochilus sandvicensis and Liparis hawaiensis. I compared these values with those of surrounding vegetation and their temperate relatives. I found that A. sandvicensis was consistently enriched in the heavy isotope of nitrogen (15N) and had higher nitrogen (N) concentrations than surrounding vegetation, and these values were even higher than those of its temperate relatives. Carbon stable isotope composition among populations of A. sandvicensis varied by island. These results point to local environment and evolutionary history determining the ecophysiology of this species. Whereas L.hawaiensis was also enriched in 15N and had on average higher N concentrations than surrounding vegetation, these values were not significantly different from temperate relatives, indicating that evolutionary history may be a stronger predictor of this orchid species' ecophysiology than environment. I suggest that both Hawaiian species are potentially partially mycoheterotrophic.

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Seed propagation and re-introduction of the U.S. federally endangered Hawaiian endemic, Platanthera holochila (Hbd.) Krzl. (Orchidaceae)

Cited by 6 publications

References 4 publications

Cyrtopodium paludicolum germination with two Tulasnella isolates

Cyrtopodium paludicolum germination with two Tulasnella isolates

Techniques for the collection, transportation, and isolation of orchid endophytes from afar: a case study from Madagascar

The carbon and nitrogen ecophysiologies of two endemic tropical orchids mirrors those of their temperate relatives and the local environment

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