Seed protein electrophoresis of the wild and cultivated species of selection faba of Vicia

Abstract

The seed protein profile obtained by disc electrophoresis was found to be a diagnostic characteristic of the broad bean, V.jaba, and of its taxonomically wild relatives V. narbonensis, V. galilaea and V. hyaeniscyamus The marked difference in the seed protein profile of the cultivated species and the three wild species indicated that none of the latter can be considered as the wild progenitor of V. faba. The similarity in the profiles of V. galilaea and V. hyaeniscyamus was consistent with the morphological resemblance of these species and their similar ecological requirements.

Geographic dispersion and varietal diversity in Vicia faba L.

“…From the comparison of the electrophoretic bands at the different levels considered (populations, groups of populations from different geographical origin and groups of populations belonging to different botanical varieties), we can conclude that ; (1) The populations studied do not seem to be very distant from each other whichever the method (Jaccard's coefficients or PCA) used to estimate the distances . This result agrees with those found by other researchers who have studied the A intraspecific biochemical variation of Vicia faba (Bassiri & Rouhani, 1977 ;Ladizinsky, 1975b ;Abdalla & Gunzel, 1979 ;Gates & Boulter, 1980;Stegemann et al ., 1980) . (2) The analysis performed at the varietal level showed that three botanical varieties (major, equina and minor) were very close to each other ; Ladizinsky (1975b), Abdalla & Gunzel, (1979) and Fayed (1989) found no differences among these botanical varieties in terms of biochemical variation .…”

“…This result agrees with those found by other researchers who have studied the A intraspecific biochemical variation of Vicia faba (Bassiri & Rouhani, 1977 ;Ladizinsky, 1975b ;Abdalla & Gunzel, 1979 ;Gates & Boulter, 1980;Stegemann et al ., 1980) . (2) The analysis performed at the varietal level showed that three botanical varieties (major, equina and minor) were very close to each other ; Ladizinsky (1975b), Abdalla & Gunzel, (1979) and Fayed (1989) found no differences among these botanical varieties in terms of biochemical variation .…”

“…Ladizinsky (1975b), on the contrary, in a study of 90 accessions belonging to the major, equina and minor groups, originated in a wide geographical area, did not find any relationship between variation in the seed protein profiles and their geographical distribution . Polignano et al .…”

“…Although considerable systematic work has been done in Vicia faba, the variability of both protein profiles (Ladizinsky, 1975b ;Abdalla & Gunzel, 1979) and isozymes in this species has not yet been extensively examined, although there has recently been detected a new interest in the subject (Polignano et al ., 1986(Polignano et al ., , 1990(Polignano et al ., , 1991 . Also scarce are the studies on isozymes (Gates & Boulter, 1980 ;Suso & Moreno, 1982 ;De Mora, 1985 ;Mancini et al ., 1989 ;Suso et al ., 1993), which, to our knowledge, have not been applied until now in a systematic way to evolutionary studies in this species .…”

“…(1990) suggested that the different patterns found in the areas they studied can be explained by different evolutionary processes in these regions . The complete lack of variation in protein profiles found by Ladizinsky (1975b) could be attributed not only to the sample size but also the aim of his study, i .e ., to compare the protein profiles of V. faba with those of some putative ancestor; his study was based on certain diagnostic bands rather than on electrophoretic patterns showing intraspecific variation . Thus, it may be very likely that the process of varietal diversification has not been accompanied by a parallel genetic divergence at the isozymic level .…”