Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

Abstract

Total seed proteins of 24 common buckwheat cultivars and cultivated populations within a molecular weight range of 30 to 54 kDa were analysed by SDS-PAGE. Single seed analysis of six cultivars identified a total of 18 alternative protein bands with different mobilities. Differences of individual protein band frequencies extracted from single seeds among six buckwheat cultivars varied distinctively, indicating high intravarietal polymorphism. The relation between frequencies of protein bands revealed by single seed analysis and their appearance on the bulk seed analyses was demonstrated. Regarding to band mobility rate and relative band intensity among 24 bulk samples analysed, 14 had distinctive electrophoregrams while the other 10 were ranged into four distinct groups. Analysis of endosperm and cotyledon proteins showed that proteins stored in these main seed parts are tissue specific. The observed electrophoretic polymorphism related to proteins stored in the cotyledons while there was no apparent variability with endosperm proteins.

Genetic diversity and relationships in some Vicia species as determined by SDS-PAGE of seed proteins

“…A bulk sample is a composite seed sample that represents the mixture of electrophoretic phenotypes present in a certain cultivar, population or species (Rogal and Javornik 1996).…”

The Major Seed Storage Proteins of Spelt Wheat, Sorghum, Millets and Pseudocereals

“…These subunits appear to be located mainly in the endosperm whereas the 13S globulin is located in the cotyledons [66,75], and accumulate in the developing seed . [74].…”

Effect of environmental and genetic factors on the stability of pea (Pisum sativum L.) isozyme and DNA markers

“…A bulk sample is a composite seed sample that represents the mixture of electrophoretic phenotypes present in a cultivar. For example, the comparison between single seed and bulk electrophoregrams in buckwheat showed that a substantial part of differences among cultivars in bulk electrophoregrams can be explained by differences in the band frequencies revealed by single seed analysis and thus variation was much higher within cultivars than between them (Rogl & Javornik 1996). Similarly, Oleo et al (1992) studied intravarietal (intrapopulation) variability in sugar beet (outbreeding crop) by means of ADH, MDH, ACO enzyme analysis and observed intravarietal variation while analyzing single seeds, however, using bulk samples (20 seeds) almost completely masked the individual genotypic differences.…”