Seed‐set and pollen–stigma compatibility in <i>Leymus chinensis</i>

Zhang, W. D.; Chen, S. Y.; Liu, G. S.; Jan, C. C.

doi:10.1111/j.1365-2494.2004.00419.x

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…It is a self-incompatible species, which often enforces outcrossing and may have increased the geographic distribution of genetic diversity [9]. Sheepgrass has diverse environmental adaptations and can grow across diverse soil and climate conditions.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis in Sheepgrass (Leymus chinensis): A Dominant Perennial Grass of the Eurasian Steppe

et al. 2013

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BackgroundSheepgrass [Leymus chinensis (Trin.) Tzvel.] is an important perennial forage grass across the Eurasian Steppe and is known for its adaptability to various environmental conditions. However, insufficient data resources in public databases for sheepgrass limited our understanding of the mechanism of environmental adaptations, gene discovery and molecular marker development.ResultsThe transcriptome of sheepgrass was sequenced using Roche 454 pyrosequencing technology. We assembled 952,328 high-quality reads into 87,214 unigenes, including 32,416 contigs and 54,798 singletons. There were 15,450 contigs over 500 bp in length. BLAST searches of our database against Swiss-Prot and NCBI non-redundant protein sequences (nr) databases resulted in the annotation of 54,584 (62.6%) of the unigenes. Gene Ontology (GO) analysis assigned 89,129 GO term annotations for 17,463 unigenes. We identified 11,675 core Poaceae-specific and 12,811 putative sheepgrass-specific unigenes by BLAST searches against all plant genome and transcriptome databases. A total of 2,979 specific freezing-responsive unigenes were found from this RNAseq dataset. We identified 3,818 EST-SSRs in 3,597 unigenes, and some SSRs contained unigenes that were also candidates for freezing-response genes. Characterizations of nucleotide repeats and dominant motifs of SSRs in sheepgrass were also performed. Similarity and phylogenetic analysis indicated that sheepgrass is closely related to barley and wheat.ConclusionsThis research has greatly enriched sheepgrass transcriptome resources. The identified stress-related genes will help us to decipher the genetic basis of the environmental and ecological adaptations of this species and will be used to improve wheat and barley crops through hybridization or genetic transformation. The EST-SSRs reported here will be a valuable resource for future gene-phenotype studies and for the molecular breeding of sheepgrass and other Poaceae species.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis in Sheepgrass (Leymus chinensis): A Dominant Perennial Grass of the Eurasian Steppe

et al. 2013

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“…This can be attributed to the self-adjusting of L. chinensis for the highly adaptation to alkaline-sodic soils (pH 8.5-11.5) (Zhang et al, 2004;Jin et al, 2006;Jin et al, 2008;Chen and Wang, 2009). …”

Section: Accepted Manuscriptmentioning

confidence: 94%

“…chinensis, a native, perennial rhizomatous grass with high palatability and forage value, is one of the dominant species in grassland of northern China, including Songnen Grassland Liu et al, 2004) and part of the Inner Mongolia Steppe Wang et al, 2010). L. chinensis is capable of growing in various environments, such as drought, low temperature, and highly alkaline-sodic (pH 8.5-11.5) soils (Zhang et al, 2004;Jin et al, 2006;Jin et al, 2008;Chen and Wang, 2009). …”

Section: Introductionmentioning

confidence: 97%

Physiological Responses ofLeymus Chinensisto Long-Term Salt, Alkali and Mixed Salt-Alkali Stresses

Wang

Cheng

Wang

2015

Journal of Plant Nutrition

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The aim of this study was to evaluate the physiological responses of Leymus chinensis (Trin.)Tzvel exposed to long-term salt, salt-alkali, and alkali stress in order to elucidate how L. chinensis can survive under alkaline-sodic soils. L. chinensis (30 days after germination) were stressed with salt [SS; sodium chloride (NaCl)], mixed salt-alkali [MS; molar ratio of NaCl: sodium carbonate (Na 2 CO 3 )=2:1] and alkali salt (AS; Na 2 CO 3 ) at four different levels of sodium (Na + ) concentration (0, 75, 150, and 300 mM) for 60 days. L. chinensis showed 100% survival rate at all treatments except 300 mM SS (33.3%) and AS (18.9%). The growth and physiological parameters of survival plants were measured. As anticipated, growth of L. chinensis was inhibited after stresses, which reflected in the decline of plant height, dry weight and tiller number following the increased Na + concentration. The content of Na + , proline, and soluble sugar in L. chinensis increased with the increasing Na + concentration, suggesting that L. chinensis need to accumulate inorganic and organic solutes for resisting osmotic stress induced by various salt stresses. These processes ensure the water balance that can provide a relative normal physiological environment for L. chinensis. Potassium (K + ) content of L. chinensis kept at a relative lower level than control to ensure the normal physiological processes. Chlorophyll content of stressed plant increased slightly compared to control plants, which can produce more

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“…Our previous study revealed that sheepgrass is a GSI species, in which most self-pollinated pollen grains are incompatible but cross-pollinated pollen grains are compatible, and the seed-set range of sheepgrass ranges from 6.50% to 56.70% under cross-pollination and from 0.56% to 4.26% under self-pollination [48]. Subsequently, a transcriptomic analysis of mature stigmas, mature ovaries, and leaves were carried out and 1025 sheepgrass stigma-specific or preferentially expressed genes were identified in our laboratory [49].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility

Chen

Jia

Cheng

et al. 2019

IJMS

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Sheepgrass (Leymus chinensis (Trin.) Tzvel.) is an economically and ecologically important forage in the grass family. Self-incompatibility (SI) limits its seed production due to the low seed-setting rate after self-pollination. However, investigations into the molecular mechanisms of sheepgrass SI are lacking. Therefore, microscopic observation of pollen germination and pollen tube growth, as well as transcriptomic analyses of pistils after self- and cross-pollination, were performed. The results indicated that pollen tube growth was rapidly inhibited from 10 to 30 min after self-pollination and subsequently stopped but preceded normally after cross-pollination. Time course comparative transcriptomics revealed different transcriptome dynamics between self- and cross-pollination. A pool of SI-related signaling genes and pathways was generated, including genes related to calcium (Ca2+) signaling, protein phosphorylation, plant hormone, reactive oxygen species (ROS), nitric oxide (NO), cytoskeleton, and programmed cell death (PCD). A putative SI response molecular model in sheepgrass was presented. The model shows that SI may trigger a comprehensive calcium- and phytohormone-dominated signaling cascade and activate PCD, which may explain the rapid inhibition of self-pollen tube growth as observed by cytological analyses. These results provided new insight into the molecular mechanisms of sheepgrass (grass family) SI.

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Seed‐set and pollen–stigma compatibility in Leymus chinensis

Cited by 12 publications

References 22 publications

Transcriptome Analysis in Sheepgrass (Leymus chinensis): A Dominant Perennial Grass of the Eurasian Steppe

Transcriptome Analysis in Sheepgrass (Leymus chinensis): A Dominant Perennial Grass of the Eurasian Steppe

Physiological Responses ofLeymus Chinensisto Long-Term Salt, Alkali and Mixed Salt-Alkali Stresses

Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility

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