Experiments were conducted to examine the effects of cocultivating the important bioenergy crop switchgrass with the ectomycorrhizal fungus Sebacina vermifera under severe drought conditions. Plants cocultivated with the fungus produced significantly higher biomass and had a higher macronutrient content than uninoculated control plants under both adequately watered and drought conditions. Drought is a predominant factor limiting plant growth and yield in both dry land and irrigated agriculture (6,27). Lack of soil water has a wide range of effects on morphological and biochemical processes in plants, including nutrient uptake from the soil, negatively impacting crop productivity (2). Unfortunately, the influence of drought on agriculture is expected to worsen in the future due to climate change (8) and increasing demands for water for municipal and residential consumption (9). Thus, various strategies are being developed to maximize water use efficiency and minimize the effects of drought on agriculture (3,12,14,18,23,26,29). However, utilization of naturally occurring symbiotic microbes to enhance drought tolerance of agricultural crops has remained largely unexplored.Most plant species in natural ecosystems are in symbiotic relationships with mycorrhizal and/or endophytic fungi (21). Members of the newly defined basidiomycete order Sebacinales naturally form a wide spectrum of mycorrhizal types of relationships (31) with the roots of various mono-and dicotyledonous plants (4,11,15,28,30). Two species in particular, Sebacina vermifera [Serendipita vermifera (Oberw.) P. Roberts, comb. nov] and its close relative Piriformospora indica, have stimulated considerable attention over the past several years, because they form endophytic and mycorrhiza-like associations with most plant species studied to date (30,32). This is of great interest, because both species are axenically cultivable, possess plant growth-promoting characteristics, and contribute several other benefits to their host plants (4,11,15,28,30). Two previous studies have shown that colonization of roots by P. indica confers drought tolerance in Arabidopsis thaliana and Chinese cabbage (24, 25). However, no similar studies have been performed to evaluate the potential of S. vermifera to impart drought tolerance to host plants. Our objective here was to investigate the effect of S. vermifera in mitigating biomass losses in switchgrass due to drought, with the ultimate goal of maximizing the utility of this important bioenergy crop and the range of lands upon which it can be grown.An in vitro study was performed using 175-ml plant containers (65 mm in diameter by 65 mm in height) with lids. The containers were filled with 25 ml of modified PNM culture medium (24) and overlaid with a nylon disk (mesh pore size, 50 m). Two strains of S. vermifera, MAFF-305828 and MAFF-305830, were used in the study. One 5-mm-diameter plug of fungal hyphae from a colony actively growing on malt extract agar (MEA) was placed at the center of the nylon disk and allowed to grow...