Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

Koziol, Adam; Loit, Evelin; McNulty, Melissa; MacFarlane, Amanda J.; Scott, Fraser W.; Altosaar, Illimar

doi:10.1186/1756-0500-5-385

Cited by 11 publications

(8 citation statements)

References 58 publications

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“…Analysis of sequence coverage indicated that these spots were produced by cleavage of the N-termini of these proteins. These results are consistent with previous investigations indicating that globulin-3 family proteins can be processed at internal cleavage sites to yield polypeptides with a large range of molecular weights and pI values (Singh et al 2001;Dupont et al 2011;Koziol et al 2012). Interestingly, the spots corresponding to full-length globulin were downaccumulated under e[CO 2 ], whereas those corresponding to endo-proteolytic events accumulated or appeared in samples grown under these conditions.…”

Section: Changes In Seed Proteome Profilessupporting

confidence: 92%

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

Verrillo

Badeck²,

Terzi³

et al. 2017

Crop Pasture Sci.

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Abstract. The aim of this study was to investigate the impact of elevated concentration of carbon dioxide (CO 2 ), as expected over coming decades, on yield and quality of winter bread wheat (Triticum aestivum L.). Plants (cv. Bologna) were grown by using the free-air CO 2 enrichment (FACE) system at Fiorenzuola d'Arda under ambient (control) and elevated (570 ppm, e[CO 2 ]) CO 2 concentrations for two growing seasons. We addressed whether there would be a response of wheat grains to elevated CO 2 concentration in terms of the contents of nitrogen (N), micro-and macronutrients, proteins and free amino acids. Under e[CO 2 ], total wheat biomass and grain yield increased in both years of the study. Grain N percentage was reduced under e[CO 2 ], but grain N yield (kg ha -1 ) was increased. Among macro-and micronutrients, a decrease in zinc concentration was observed. The proteome pattern was significantly different in grains grown at the two different CO 2 levels, but the observed changes were highly dependent on interactions with prevailing environmental conditions. Finally, a negative trend was observed in the early germination rates of seeds from plants grown under e[CO 2 ] compared with the controls. The results suggest that the expected increase in CO 2 levels and their interactive effects with environmental variables may influence agronomic performance by increasing yield and negatively affecting quality.

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Section: Changes In Seed Proteome Profilessupporting

confidence: 92%

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

Verrillo

Badeck²,

Terzi³

et al. 2017

Crop Pasture Sci.

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“…All the 15 significantly changed globulins in JB Asano were increased while most of which in Tobak was decreased by split N application. The increases in globulins abundance may reduce the nutritional quality of wheat flour since globulin-3 has been associated with the incidence of diabetes (Koziol et al, 2012). Moreover, wheat globulins are also likely to be glycosylated which may result in improved emulsifying capacity and emulsion stability (Altenbach et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Protein Composition and Baking Quality of Wheat Flour as Affected by Split Nitrogen Application

et al. 2019

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Baking quality of wheat flour is determined by grain protein concentration (GPC) and its composition and is highly influenced by environmental factors such as nitrogen (N) fertilization management. This study investigated the effect of split N application on grain protein composition and baking quality of two winter wheat cultivars, Tobak and JB Asano, belonging to different baking quality classes. Bread loaf volumes in both cultivars were enhanced by split N application. In contrast, GPC was only significantly increased in JB Asano. Comparative 2-DE revealed that the relative volumes of 21 and 28 unique protein spots were significantly changed by split N application in Tobak and JB Asano, respectively. Specifically, the alterations in relative abundance of certain proteins, i.e., globulins, LMW-GS, α-, and γ-gliadins as well as α-amylase/trypsin inhibitors were more sensitive to split N application. Furthermore, certain proteins identified as globulins and alpha-amylase inhibitors were changed in both wheat cultivars under split N application. These results implied that the functions of these unique proteins might have played important roles in affecting baking quality of wheat flour, especially for cultivars (i.e., Tobak in the present study) the baking quality of which is less dependent on GPC.

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“…In wheat, globulin-3 was cleaved post-translationally in embryos. Five major polypeptide spots of globulin-3 were identified by MS and N-terminal sequencing, and each spot displayed different Mr and pI, these post-translational processing events that lead to the maturation of the globulin family of proteins observed seed protein fraction that could be associated with type 1 diabetes or celiac disease following endoproteolytic processing ( Koziol et al, 2012 ). In barley, globulin contains 637 amino acids with one signal peptide detected by the SignalP v4.0 program, the peptide starts at position 1 and ends at position 27.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

et al. 2016

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Grain protein contents (GPCs) of barley seeds are significantly different between feed and malting barley cultivars. However, there is still no insight into the proteomic analysis of seed proteins between feed and malting barley cultivars. Also, the genetic control of barley GPC is still unclear. GPCs were measured between mature grains of Yangsimai 3 and Naso Nijo. A proteome profiling of differentially expressed protein was established by using a combination of 2-DE and tandem mass spectrometry. In total, 502 reproducible protein spots in barley seed proteome were detected with a pH range of 4–7 and 6–11, among these 41 protein spots (8.17%) were detected differentially expressed between Yangsimai 3 and Naso Nijo. Thirty-four protein spots corresponding to 23 different proteins were identified, which were grouped into eight categories, including stress, protein degradation and post-translational modification, development, cell, signaling, glycolysis, starch metabolism, and other functions. Among the identified proteins, enolase (spot 274) and small subunit of ADP-glucose pyrophosphorylase (spot 271) are exclusively expressed in barley Yangsimai 3, which may be involved in regulating seed protein expression. In addition, malting quality is characterized by an accumulation of serpin protein, Alpha-amylase/trypsin inhibitor CMb and Alpha-amylase inhibitor BDAI-1. Most noticeably, globulin, an important storage protein in barley seed, undergoes post-translational processing in both cultivars, and also displays different expression patterns.

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Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

Cited by 11 publications

References 58 publications

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

Protein Composition and Baking Quality of Wheat Flour as Affected by Split Nitrogen Application

Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

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