Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean

Kumar, Awadhesh; Kumar, Varun; Krishnan, Veda; Hada, Alkesh; Marathe, Ashish; Parameswaran, C.; Jolly, M. S.; Sachdev, Archana

doi:10.1038/s41598-019-44255-7

Cited by 34 publications

(14 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In conclusion, our work confirms that normal InsP 6 biosynthesis is important in maintaining basal defences against pathogens and also shows that it contributes to more than one defensive mechanism. In creating low-phytate crops, it may be wise not to inhibit synthesis of InsP 6 in all tissues but to do so only in those parts that are to be consumed in the human diet or processed as animal feeds, an approach shown to be feasible in, for example, soybean where levels of this metabolite were selectively decreased in the seeds (Kumar et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Inositol hexakisphosphate biosynthesis underpins PAMP‐triggered immunity to Pseudomonas syringae pv. tomato in Arabidopsis thaliana but is dispensable for establishment of systemic acquired resistance

Poon

Fevre

Carr

et al. 2019

Molecular Plant Pathology

View full text Add to dashboard Cite

Phytic acid (inositol hexakisphosphate, InsP6) is an important phosphate store and signal molecule necessary for maintenance of basal resistance to plant pathogens. Arabidopsis thaliana (‘arabidopsis’) has three genes encoding myo‐inositol phosphate synthases (IPS1–3), the enzymes that catalyse conversion of glucose‐6‐phosphate to InsP, the first step in InsP6 biosynthesis. There is one gene for inositol‐(1,3,4,5,6)‐pentakisphosphate 2‐kinase (IPK1), which catalyses the final step. Previously, we showed that mutation of IPS2 and IPK1 but not IPS1 increased susceptibility to pathogens. Our aim was to better understand the InsP6 biosynthesis pathway in plant defence. Here we found that the susceptibility of arabidopsis (Col‐0) to virulent and avirulent Pseudomonas syringae pv. tomato was also increased in ips3 and ips2/3 double mutants. Also, ipk1 plants had compromised expression of local acquired resistance induced by treatment with the pathogen‐derived molecular pattern (PAMP) molecule flg22, but were unaffected in other responses to flg22, including Ca2+ influx and the oxidative burst, seedling root growth inhibition, and transcriptional up‐regulation of the PAMP‐triggered genes MITOGEN‐ACTIVATED PROTEIN KINASE (MPK) 3, MPK11, CINNAMYL ALCOHOL DEHYDROGENASE 5, and FLG22‐INDUCED RECEPTOR‐LIKE KINASE 1. IPK1 mutation did not prevent the induction of systemic acquired resistance by avirulent P. syringae. Also, ips2 and ips2/3 double mutant plants, like ipk1, were hypersusceptible to P. syringae but were not compromised in flg22‐induced local acquired resistance. The results support the role of InsP6 biosynthesis enzymes in effective basal resistance and indicate that there is more than one basal resistance mechanism dependent upon InsP6 biosynthesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Inositol hexakisphosphate biosynthesis underpins PAMP‐triggered immunity to Pseudomonas syringae pv. tomato in Arabidopsis thaliana but is dispensable for establishment of systemic acquired resistance

Poon

Fevre

Carr

et al. 2019

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…These genes were silenced in seeds using seed-specific promoters that resulted in 68% reduction in PA accumulation with no adverse effect on seed germination or plant growth. Similarly, low PA transgenic lines were developed in soybean by silencing genes inositol polyphosphate 6-/3-/5-kinase (IPK2) and myo-inositol-3phosphate synthase (MIPS) with significant reduction in seed PA and increase in Fe, Zn, and Ca content (Punjabi et al 2018;Kumar et al 2019). It may take another 6-8 years to develop crop varieties with low PA and increased micronutrients without compromising on plant growth and grain yield either through conventional breeding or transgenic approaches.…”

Section: Approaches To Improve Nutritional Quality By Reducing Phytatmentioning

confidence: 99%

Exploring phosphorus fertilizers and fertilization strategies for improved human and environmental health

2020

View full text Add to dashboard Cite

Mineral phosphorus (P) fertilizers support high crop yields and contribute to feeding the teeming global population. However, complex edaphic processes cause P to be immobilized in soil, hampering its timely and sufficient availability for uptake by plants. The resultant low use efficiency of current water-soluble P fertilizers creates significant environmental and human health problems. Current practices to increase P use efficiency have been inadequate to curtail these problems. We advocate for the understanding of plant physiological processes, such as physiological P requirement, storage of excess P as phytate, and plant uptake mechanisms, to identify novel ways of designing and delivering P fertilizers to plants for improved uptake. We note the importance and implications of the contrasting role of micronutrients such as zinc and iron in stimulating P availability under low soil P content, while inhibiting P uptake under high P fertilization; this could provide an avenue for managing P for plant use under different P fertilization regimes. We argue that the improvement of the nutritional value of crops, especially cereals, through reduced phytic acid and increased zinc and iron contents should be among the most important drivers toward the development of innovative fertilizer products and fertilization technologies. In this paper, we present various pathways in support of this argument. Retuning P fertilizer products and application strategies will contribute to fighting hunger and micronutrient deficiencies in humans. Moreover, direct soil P losses will be reduced as a result of improved P absorption by plants.

show abstract

“…The estimation of PA was done by an assay procedure which involved the measurement of P from PA after digestion with phytase and alkaline phosphatase enzyme using Phytic acid assay kit (Megazyme International Limited, Ireland) with some modifications (Kumar et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

“…The bioavailability of Fe and Zn in the rice grain samples was estimated as per modified in vitro protocol (Kumar et al., 2019; Liang et al., 2008). A powdered rice grain sample of about 5 g was suspended in 30 ml distilled water for ~10 hr in a water bath shaker (150 rpm).…”

Section: Methodsmentioning

confidence: 99%

A single nucleotide substitution in the SPDT transporter gene reduced phytic acid and increased mineral bioavailability from Rice grain ( Oryza sativa L.)

et al. 2021

Self Cite

View full text Add to dashboard Cite

Phosphorus (P) flow in agricultural land depends on the P taken off from harvested product, its losses through runoff and fertilizer applied to balance the removed P. Phytic acid (PA), the major storage form of phosphorus (P) in cereal grains is a key anti‐nutrient for human and non‐ruminants leads to eutrophication of waterways. As the natural non‐renewable P reserves are limited, enhancing P use efficiency is needed for field crops. SULTR‐like phosphorus distribution transporter (SPDT) is a novel rice transporter transfer P to the grain. Any alteration in transporter gene reduce grain P with concomitant rise in the leaves. A low PA (3.0 g/kg) rice Khira was identified where a single nucleotide mutation in LOC_Os06g05160 gene encoding SPDT showed low P transportation to grain. An amino acid change was detected as Valine‐330 to Alanine at the 3′ end of fifth exon. Highest expression of SPDT was observed in node I of rice as compared to low PA genotype. The mutation in SPDT could significantly affect P and PA accumulation in the grains with increased mineral bioavailability. Practical applications Excessive P application in crop leads to higher production cost as well as rapid depletion of limited rock phosphate. Alteration of P transporter function in the rice lower PA and total P accumulation in the grains with increased mineral bioavailability. The re‐distributed P in the straw can be applied as manure to the rice field. Thus, less P will be removed from the field, result in the decreased requirement for P fertilizer.

show abstract

Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean

Cited by 34 publications

References 46 publications

Inositol hexakisphosphate biosynthesis underpins PAMP‐triggered immunity to Pseudomonas syringae pv. tomato in Arabidopsis thaliana but is dispensable for establishment of systemic acquired resistance

Inositol hexakisphosphate biosynthesis underpins PAMP‐triggered immunity to Pseudomonas syringae pv. tomato in Arabidopsis thaliana but is dispensable for establishment of systemic acquired resistance

Exploring phosphorus fertilizers and fertilization strategies for improved human and environmental health

A single nucleotide substitution in the SPDT transporter gene reduced phytic acid and increased mineral bioavailability from Rice grain ( Oryza sativa L.)

Contact Info

Product

Resources

About