Seed transmission of Cucumber mosaic virus in pepper

Ali, Akhtar; Kobayashi, Michelle

doi:10.1016/j.jviromet.2009.09.026

Cited by 69 publications

(46 citation statements)

References 16 publications

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“…Such a rapid evolution could be, at least in part, a consequence of the severe bottlenecks experienced by PSbMV populations during vertical transmission. Similar declines in virulence [46] or symptom induction [37], [47], [48] have been observed for other seed-transmitted plant viruses.…”

Section: Discussionsupporting

confidence: 71%

Narrow Bottlenecks Affect Pea Seedborne Mosaic Virus Populations during Vertical Seed Transmission but not during Leaf Colonization

Fabre¹,

Moury²,

Johansen

et al. 2014

PLoS Pathog

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The effective size of populations (Ne) determines whether selection or genetic drift is the predominant force shaping their genetic structure and evolution. Populations having high Ne adapt faster, as selection acts more intensely, than populations having low Ne, where random effects of genetic drift dominate. Estimating Ne for various steps of plant virus life cycle has been the focus of several studies in the last decade, but no estimates are available for the vertical transmission of plant viruses, although virus seed transmission is economically significant in at least 18% of plant viruses in at least one plant species. Here we study the co-dynamics of two variants of Pea seedborne mosaic virus (PSbMV) colonizing leaves of pea plants (Pisum sativum L.) during the whole flowering period, and their subsequent transmission to plant progeny through seeds. Whereas classical estimators of Ne could be used for leaf infection at the systemic level, as virus variants were equally competitive, dedicated stochastic models were needed to estimate Ne during vertical transmission. Very little genetic drift was observed during the infection of apical leaves, with Ne values ranging from 59 to 216. In contrast, a very drastic genetic drift was observed during vertical transmission, with an average number of infectious virus particles contributing to the infection of a seedling from an infected mother plant close to one. A simple model of vertical transmission, assuming a cumulative action of virus infectious particles and a virus density threshold required for vertical transmission to occur fitted the experimental data very satisfactorily. This study reveals that vertically-transmitted viruses endure bottlenecks as narrow as those imposed by horizontal transmission. These bottlenecks are likely to slow down virus adaptation and could decrease virus fitness and virulence.

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Section: Discussionsupporting

confidence: 71%

Narrow Bottlenecks Affect Pea Seedborne Mosaic Virus Populations during Vertical Seed Transmission but not during Leaf Colonization

Fabre¹,

Moury²,

Johansen

et al. 2014

PLoS Pathog

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“…Transmission of viruses through seeds, even at very low rates, is important for the spread, overwintering, and longdistance dissemination of viruses. Seed transmission of a virus provides an initial source of inoculum for the transmission by the viral vector and may have a considerable impact on crop yields (Ali & Kobayashi, 2010). To date, there is no report of seed transmission for any geminivirus including SPLCV (Rojas & Gilbertson, 2008).…”

Section: Introductionmentioning

confidence: 99%

Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)

et al. 2015

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Sweet potato leaf curl virus (SPLCV) infects sweet potato and is a member of the family Geminiviridae (genus Begomovirus). SPLCV transmission occurs from plant to plant mostly via vegetative propagation as well as by the insect vector Bemisia tabaci. When sweet potato seeds were planted and cultivated in a whitefly‐free greenhouse, some sweet potato plants started to show SPLCV‐specific symptoms. SPLCV was detected by PCR from all leaves and floral tissues that showed leaf curl disease symptoms. More than 70% of the seeds harvested from SPLCV‐infected sweet potato plants tested positive for SPLCV. SPLCV was also identified from dissected endosperm and embryos. The transmission level of SPLCV from seeds to seedlings was up to 15%. Southern blot hybridization showed SPLCV‐specific single‐ and double‐stranded DNAs in seedlings germinated from SPLCV‐infected seeds. Taken altogether, the results show that SPLCV in plants of the tested sweet potato cultivars can be transmitted via seeds and SPLCV DNA can replicate in developing seedlings. This is the first seed transmission report of SPLCV in sweet potato plants and also, to the authors' knowledge, the first report of seed transmission for any geminivirus.

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“…TMV is transmitted through the seed coat and is transmitted endosperm in tomato seeds, but not through the embryo (Broadbent, 1965) and through seed coat, rarely the endosperm or embryo in pepper seeds (Demski 1981). CMV is present in the embryo, endosperm as well as the testa in pepper, melon and zucchini seeds (Ali and Kobayashi 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Treatments on the Inactivation of Various Seedborne Viruses in Some Vegetables

Paylan

Erkan

Çetinkaya

et al. 2014

Ozone: Science & Engineering

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In this study, we considered the efficacy of seed treatments for the inactivation of some seedborne viruses in tomato, pepper, melon, squash, bean and lettuce seeds, which are essential for human nutrition and seed production in our country. A total of 325 seed samples obtained from various farmers and foundations were tested by DAS-ELISA and RT-PCR procedures. Eight seed lots infected with Tomato mosaic tobamovirus (ToMV), Tobacco mosaic tobamovirus (TMV), Cucumber mosaic cucumovirus (CMV), Soybean mosaic potyvirus (SMV) and Lettuce mosaic potyvirus (LMV) were selected as research materials. Virus inactivation treatments were made by using acetic acid (CH 3 COOH), hydrogen peroxide (H 2 O 2 ), hydrochloric acid (HCl), sodium hypochlorite (NaClO), Triton X 100, dry heat, heated water, ozone (O 3 ), and UV (305 nm wavelength). The most effective treatments for reducing virus concentration were HCl, heated water (65 • C) and ozone (10 g m −3 ). These treatments reduced concentrations of seed-borne viruses in ranges of 51%, 42%, and 32%, respectively. Other treatments were less effective and reduced virus concentrations in the range of 27%-12%. HCl and ozone treatments were the most effective and applicable methods because they did not have negative effect on seed germination.

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Seed transmission of Cucumber mosaic virus in pepper

Cited by 69 publications

References 16 publications

Narrow Bottlenecks Affect Pea Seedborne Mosaic Virus Populations during Vertical Seed Transmission but not during Leaf Colonization

Narrow Bottlenecks Affect Pea Seedborne Mosaic Virus Populations during Vertical Seed Transmission but not during Leaf Colonization

Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)

Effects of Different Treatments on the Inactivation of Various Seedborne Viruses in Some Vegetables

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