Seed Viability and Symbiotic Seed Germination in Vanilla spp. (Orchidaceae)

Alomía, Yasmin A.; Mosquera-E, A.T.; Flanagan, Nicola S.; Otero, J. Túpac

doi:10.3923/rjss.2017.43.52

Cited by 16 publications

(11 citation statements)

References 25 publications

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“…There are many different culture media use for asymbiotic germination of orchid, including MS, VW, Kundson (KC) and NDM. Like the seeds of other orchid species, Vanilla species produce many small seeds without endosperm, they have low germination rate under natural conditions and as such there is a nutrient requirement for initiation of germination [17,18]. The results from this study showed that NDM medium provided a good germination rate of V. siamensis seed while prolonging survival rate of the protocorm probably due to the high contents of mineral in medium.…”

Section: Discussionmentioning

confidence: 67%

In vitro seed germination and plantlet regeneration of Vanilla siamensis: An endemic species in Thailand

Chaipanich¹,

Roberts²,

Yenchon³

et al. 2020

ScienceAsia

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This study reports the in vitro germination of self-pollinated pod of Vanilla siamensis, native to Thailand. The aim of the present work is to determine the effect of culture media and plant growth regulators on seed germination and plantlet regeneration. The vanilla seeds were asymbiotically germinated on different culture media under aseptic conditions. The results showed that, New Dogashima medium (NDM) supplemented with 2% (w/v) sucrose, 15% (v/v) coconut water (CW) and 0.7% (w/v) agar gave the highest levels of seed germination at 10.1%. The fastest and highest percentage seed germination was achieved using NDM supplemented with 2 mg/l gibberellic acid (GA 3). Seeds on this culture medium germinated within 7-8 weeks in comparison with 10-11 weeks on culture medium without plant growth regulators. Protocorms were transferred to different culture media with various concentrations of 6benzyladenine (BA) to assess the protocorm development. The results revealed that ½ Murashige and Skoog (½ MS) medium gave the higher result in survival rate of protocorm than NDM. ½ MS medium with 0.5 mg/l BA was suitable for shoot formation. Plantlet development was completed after subculturing on ½ MS medium supplemented with 0.5 mg/l α-naphthaleneacetic acid (NAA) for 8 weeks and successfully acclimatized at 91.7%. The protocol outline here can be used for mass propagation of this endangered species and used as a guideline for improvement of V. siamensis and its in vitro conservation.

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Section: Discussionmentioning

confidence: 67%

In vitro seed germination and plantlet regeneration of Vanilla siamensis: An endemic species in Thailand

Chaipanich¹,

Roberts²,

Yenchon³

et al. 2020

ScienceAsia

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“…Crop wild relatives have long been recognized as priority species for conservation because their extinction threatens the genetic base of many of the world's crops (Heywood , Castañeda‐Álvarez ). Their protection started with ex situ conservation programs, which were not as successful as expected, especially for species that are characterized by small and dispersed, genetically distinct populations with low seed viability and complex interspecific relationships within their natural habitat, which is the case for vanilla species (Alomia , Azofeifa‐Bolaños et al. ).…”

Section: Discussionmentioning

confidence: 99%

Vanilla distribution modeling for conservation and sustainable cultivation in a joint land sparing/sharing concept

et al. 2020

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Vanilla, an expensive but popular spice used in many industries, faces problems related to its supply. Some of these problems are due to the fact that vanilla cultivation is based on clonal material of a single species (Vanilla planifolia) and is dominated by just a few countries located outside the native growing areas of aromatic vanilla species, which is the neotropics. Despite the economic importance of this crop, relatively little attention has been paid to its wild relatives, in particular with respect to their biology, ecology, and potential use. We hypothesized that species distribution models (SDMs) can identify suitable areas for both the conservation and cultivation of vanilla crop wild relatives (CWRs), following a joint land sparing/land sharing (SPASHA) approach, thus offering alternative sourcing areas and production methods. This is the first study that explored the use of ensemble SDMs to provide applicable land use maps related to the conservation and sustainable cultivation of wild vanilla species in Costa Rica, contributing to a solution for the problems related to current vanilla production systems. We focused on four aromatic vanilla CWRs, native to Costa Rica, to make land use policy recommendations for this country, and more specifically for the biological corridor Osa and its surroundings within our study region Area de Conservaci on Osa (ACOSA). The resulting distribution maps, with a mean AUC of 0.89, reflected their current potential distribution (ranging from unsuitable to suitable) in Costa Rica. Combining them with recent land use and conservation area maps of our study region, we defined (1) areas for vanilla conservation and (2) areas for sustainable vanilla cultivation within agroforestry systems. These land use recommendations can now be integrated within the National Bio-Corridor Program (PNCB) that aims at making biological corridors more productive by proposing alternative income generation for local communities living within these areas. Our approach can be applied to identify priority areas for implementing the SPASHA approach on other vanilla CWRs and in more regions across its native growing ranges, given the availability of land use maps and enough occurrence records to build accurate SDMs.

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“…According to Table 1 , many orchids are considered generalist—or anecdotally, promiscuous—forming OM with fungi isolated from another orchid species [ 6 , 48 , 56 , 57 , 58 , 59 , 60 ]. For specialists, there are no other options but to use OMF isolated from respective plants [ 61 ].…”

Section: Through the Looking Glass: How Orchids Naturally Establismentioning

confidence: 99%

In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

Pujasatria

Miura

Kaminaka

2020

Plants

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As one of the largest families of flowering plants, Orchidaceae is well-known for its high diversity and complex life cycles. Interestingly, such exquisite plants originate from minute seeds, going through challenges to germinate and establish in nature. Alternatively, orchid utilization as an economically important plant gradually decreases its natural population, therefore, driving the need for conservation. As with any conservation attempts, broad knowledge is required, including the species’ interaction with other organisms. All orchids establish mycorrhizal symbiosis with certain lineages of fungi to germinate naturally. Since the whole in situ study is considerably complex, in vitro symbiotic germination study is a promising alternative. It serves as a tool for extensive studies at morphophysiological and molecular levels. In addition, it provides insights before reintroduction into its natural habitat. Here we reviewed how mycorrhiza contributes to orchid lifecycles, methods to conduct in vitro study, and how it can be utilized for conservation needs.

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Seed Viability and Symbiotic Seed Germination in Vanilla spp. (Orchidaceae)

Cited by 16 publications

References 25 publications

In vitro seed germination and plantlet regeneration of Vanilla siamensis: An endemic species in Thailand

In vitro seed germination and plantlet regeneration of Vanilla siamensis: An endemic species in Thailand

Vanilla distribution modeling for conservation and sustainable cultivation in a joint land sparing/sharing concept

In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

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