Tomatoes are widely used worldwide and are an economically important crop. Tomatoes contain important secondary metabolites, as well as lycopene, β-carotene, vitamin C, and flavonoids. The quality of tomato seedlings varies depending on certain environmental factors during the seedling growth season. In Korea, diniconazole (DIN), a triazole-based plant growth regulator (PGR), is widely used to prevent seedling overgrowth. Misapplication of a triazole-based PGR results in excessive growth inhibition. However, little is known about the prevention of side effects such as growth inhibition after a DIN treatment. Therefore, this study sought to determine whether growth inhibition could be resolved by treatment with GA 3 , a phytohormone, after a DIN treatment. This study compared the survival rates of seedlings after a DIN treatment and analyzed the growth of tomato seedlings treated with GA 3 after a DIN treatment. To this end, DIN was diluted to a concentration of 250 mg•L -1 to be treated at 3 mg per plant, and GA 3 was diluted to a concentration of 5 mg•L -1 and 10 mg•L -1 to be treated at 2.5 mL per plant. GA 3 was administered up to two times per concentration. In the foliar application of GA 3 after the DIN treatment, no difference in growth was found between the treatments at different concentrations, but there was a significant difference between plants subjected to the single and double treatments. The results of this study suggest that a GA 3 treatment at a higher frequency than a high-concentration treatment can more effectively address the growth inhibition issue associated with a DIN treatment.