Seeds as sources of L-Dopa

Me, Daxenbichler; Ch, V; Ea, Hallinan; Fr, Earle; As, Barclay

doi:10.1021/jm00287a030

Cited by 64 publications

(30 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initial browning/blackening of explants due to leaching and subsequent oxidation of polyphenols might be responsible for delayed response in IZE explants. Baptista and Lupinus (Daxenbichler et al, 1971), in Mucuna L-Dopa accumulation is predominant in seed (3 -6%), while other parts such as stem, leaf and root also exhibits its presence at lower levels (Szabo and Tebett, 2000). L-Dopa is synthesized from tyrosine precursor in one step conversion process catalyzed by the enzyme tyrosine hydroxylase (Griffith and Conn, 1973) and in turn acts as precursor for the black pigment melanin (Brain, 1976).…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in Mucuna pruriens

S¹,

Sathyanarayana²

2009

Afr. J. Biotechnol.

View full text Add to dashboard Cite

This study reports the induction of somatic embryos in Mucuna pruriens. Different explants cultured on MS medium supplemented with 11.31 µM 2,4-D produced golden yellow embryogenic callus that induced synchronized embryo development on MS basal liquid medium. Organization of pre-embryonic mass was noticed 15 d after sub culturing the callus, which progressively developed to globular, heart, torpedo and cotyledonary shaped embryos. Attempts to germinate them did not succeed as the cotyledonary embryos turned characteristic red and subsequently brown before finally turning black and unresponsive. The reaction was consistent across different media constituents, hormonal concentrations and pH ranges. Supplementation of anti-oxidants was also ineffective. Polyacrylamide gel electrophoretic analysis of the brown and non-brown embryos revealed quantitative differences in protein contents between the two. More detailed study is necessary to establish the precise cause for embryo browning and ways of its regulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in Mucuna pruriens

S¹,

Sathyanarayana²

2009

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…When various concentrations of CuSO 4 were added as elicitors, maximum yield of L-DOPA, 9.72% (dry weight) was observed at 20 mg/dl (Fig. 2a) and in case of CaCl 2 maximum yield of 8.62% (dry weight) (Fig.…”

Section: Effect Of Elicitors On L-dopamentioning

confidence: 98%

“…3 Plants were exploited as an alternate source for the isolation of L-DOPA and in a screening survey, more than 1000 species in 135 plant families have been screened. 4 Among them, genus Mucuna ( Leguminosae ) was found to contain the maximum level of L-DOPA which was successfully exploited commercially. Among the various species of Mucuna, M. holtonii (6.4% of dry weight) and M. pruriens (5.21% of dry weight ) were promising L-DOPA content in their seeds.…”

Section: Introductionmentioning

confidence: 99%

“…Among the various species of Mucuna, M. holtonii (6.4% of dry weight) and M. pruriens (5.21% of dry weight ) were promising L-DOPA content in their seeds. 4,5 In naturally occurring L-DOPA synthesis, tyrosinase or tyrosine hydroxylase (EC 1.14.18.1) is the key enzyme which is a copper containing oxygen oxidoreductase and its deficiency causes oculocutaneus albinism in humans. Tyrosinase catalyzes the first two steps of melanin biosynthesis pathway, namely, the conversion of L-tyrosine into L-DOPA and further conversion of L-DOPA into dopaquinone.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Innovative use of intact seeds of Mucuna monosperma Wight for improved yield of L-DOPA

Inamdar

Joshi

Jadhav

et al. 2012

Nat. Prod. Bioprospect.

View full text Add to dashboard Cite

Abstract:The drug L-DOPA has been widely used against Parkinson's disease and is extracted from plants. Due to the increasing demand of this drug, new plant sources need to be discovered in addition to the existing sources. The paper embodies results on Mucuna monosperma, which can be a promising candidate for L-DOPA. The seed powder of this plant contains 5.48% of (dry weight) the drug and when the seeds were soaked in distilled water, content was increased to 6.58%. Different elicitors when added, enhanced the drug level in seed up to 11.8%. The possible rationale behind this increase was confirmed by increase in tyrosinase activity in the seeds. Presence of L-DOPA was confirmed using various analytical techniques as HPLC, HPTLC and NMR. The work demonstrates a potential candidate plant as a source for L-DOPA when a novel method was adopted as described here.

show abstract

“…However, it has been noted that when consumed without proper treatment, Mucuna seeds have adverse effects in humans and birds (Josephine and Janardhanan, 1992;Vijayakumari et al, 1996;Dossa et al, 1998Dossa et al, /1999Del Carmen et al, 1999). Among the toxic and anti-nutritional factors identified in these seeds are tannins, trypsin inhibitors, anticoagulants and L-dopa (Bell and Janzen, 1971;Daxenbichler et al, 1971;Ravindran and Ravindran, 1988). Traditional treatments such as cooking, fermentation or germination improve the protein digestibility of seeds by decreasing the levels of some anti-nutrients (Bishnoi et al, 1994;Chau and Cheung, 1997).…”

Section: Introductionmentioning

confidence: 99%

An Evaluation of the Protein Digestibility of Flours and Derived Protein Rich Product of Three Varieties of Mucuna Pruriens (L.) From Rats (Rattus Norvegicus) Males in Growth

Théopihile¹,

Ali²,

Doloum

et al. 2017

Int J Appl Sci Biotechnol

View full text Add to dashboard Cite

The objective of the present study is to evaluate the proteinaceous digestibility of crude flours and derived protein rich product of three varieties of Mucuna pruriens of the male albino rats in growth. 48 rats have been taken randomly split up into 8 groups where 6 each according to their formulated feedings; those foods are: foods without protein (RSP), foods with casein (RC), food with the black macuna derived protein rich product (RMNc), food with the scratch macuna derived protein rich product (RMRc), food with the white macuna derived protein rich product (RMBc), food with the black macuna crude flours (RMNf), food with the scratch macuna crude flours (RMRf) et food with the white macuna crude flours (RMBf). All those foods content the same quantity of azote excepted foods without protein. Rats have been isolated in the semi metabolic cages which allow gathering the remaining of the foods supplied and the faeces to determine the parameter values of consumption. The experiment has taken 28 days. The apparent and reel protein digestibilities obtained in each food are respectively : RC (76,63±0,36% ; 95,86±0,06%), RMNc (71,20±0,77% ; 91,60±0,99%), RMRc (73,68±0,23% ; 93,49±0,85%), RMBc (71,16±0,59% ; 92,03±0,70%), RMNf (33,25±1,91% ; 53,881±1,70%), RMRf (31,3±2,40% ; 52,12±2,22%) and RMBf (31,12±4,00% ; 51,92±3,8%). The results show that derived protein rich product have ameliorated the protein digestibility and could be the alternative for the use of seeds of Mucuna pruriens to feed human beings and animals.

show abstract

Seeds as sources of L-Dopa

Cited by 64 publications

References 0 publications

Somatic embryogenesis in Mucuna pruriens

Somatic embryogenesis in Mucuna pruriens

Innovative use of intact seeds of Mucuna monosperma Wight for improved yield of L-DOPA

An Evaluation of the Protein Digestibility of Flours and Derived Protein Rich Product of Three Varieties of Mucuna Pruriens (L.) From Rats (Rattus Norvegicus) Males in Growth

Contact Info

Product

Resources

About