Seeing is believing: a visualization toolbox to enhance selection efficiency in maize genome editing

Xu, Jieting; Yin, Yuejia; Jian, Liumei; Han, Baozhu; Chen, Ziqi; Yan, Jianbing

doi:10.1111/pbi.13575

Cited by 12 publications

(7 citation statements)

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“…Other features may also be manipulated to improve Agrobacterium -mediated transformation and the fate of transformed events. For example, the subversion of host plant factors ( Pitzschke, 2013 ; Sardesai et al, 2013 ; Hwang et al, 2015 ), the reduction of vector backbone and transposon integration ( Ülker et al, 2008 ; Kim and An, 2012 ; Jupe et al, 2019 ), the increase of T-DNA transfer capacity ( Nonaka et al, 2019 ), the environmental containment of Agrobacterium when used for field applications ( Torti et al, 2021 ), the increase on transient transformation frequency ( Wang et al, 2018 ) and the use of effective tools for non-invasive monitoring of gene expression and plant transformation ( He et al, 2020 ; Xu et al, 2021 ).…”

Section: Current Status Of Maize Transformationmentioning

confidence: 99%

“…However, improvements are needed to overcome the inherent problems related to haploid induction per se ( Trentin et al, 2020 ; Jacquier et al, 2021 ). For example, CRISPR-Cas can be used before or in parallel to HI-Edit to: (1) increase the haploid induction rates by targeting genes related to high haploid induction ( Kelliher et al, 2017 ; Zhong et al, 2019 ) or genes related to the inducer exclusion ( Kelliher et al, 2019 ); (2) accelerate and accurately sort kernels/seedlings with haploid embryos from normal embryos by modifying visual traits such as anthocyanin ( Chaikam et al, 2019 ) or by integrating visible transgenic markers into the inducers ( Yu and Birchler, 2016 ; Xu et al, 2021 ; Yan et al, 2021 ) or even targeting genes involved in the oil content of seeds ( Melchinger et al, 2013 ) and fixing recessive alleles of morphological traits in donors ( Trentin et al, 2020 ). Care should be taken with desirable/undesirable agronomic traits during induction of haploid plants that would compromise the breeding programmes ( Trentin et al, 2020 ).…”

Section: Emerging Technologies For Maize Transformationmentioning

confidence: 99%

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Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

et al. 2021

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Over the past decades, advances in plant biotechnology have allowed the development of genetically modified maize varieties that have significantly impacted agricultural management and improved the grain yield worldwide. To date, genetically modified varieties represent 30% of the world’s maize cultivated area and incorporate traits such as herbicide, insect and disease resistance, abiotic stress tolerance, high yield, and improved nutritional quality. Maize transformation, which is a prerequisite for genetically modified maize development, is no longer a major bottleneck. Protocols using morphogenic regulators have evolved significantly towards increasing transformation frequency and genotype independence. Emerging technologies using either stable or transient expression and tissue culture-independent methods, such as direct genome editing using RNA-guided endonuclease system as an in vivo desired-target mutator, simultaneous double haploid production and editing/haploid-inducer-mediated genome editing, and pollen transformation, are expected to lead significant progress in maize biotechnology. This review summarises the significant advances in maize transformation protocols, technologies, and applications and discusses the current status, including a pipeline for trait development and regulatory issues related to current and future genetically modified and genetically edited maize varieties.

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Section: Current Status Of Maize Transformationmentioning

confidence: 99%

Section: Emerging Technologies For Maize Transformationmentioning

confidence: 99%

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

et al. 2021

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“…For instance, in ViMeBox, a visible marker driven by a tissue-specific promoter is introduced into CRISPR-Cas expression vectors. The ViMeBox tool may also be combined with HI-Edit (KELLIHER et al, 2019) and IMGE (WANG et al, 2019a), once ViMeBox allows the easy identification of diploids by exhibiting red visible embryos, increasing the efficiency of the process (XU et al, 2021a).…”

Section: Haploid Inductionmentioning

confidence: 99%

Genome editing: propelling the next generation of crop improvement

et al. 2021

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Climate change and population size records threaten food security. Therefore, the call for a more sustainable and efficient crop production has never been more urgent. Traditional plant breeding was one of the first successful approaches to expand cultivation areas and crop yield. Later, biotechnological tools and their products, such as genetically modified organisms containing exogenous DNA, further broadened the limits of agricultural results, yet bringing huge financial, bureaucratic, and public rejection hurdles. In the 90s, scientific advances brought the opportunity to drive mutations using engineered nucleases, and since 2013 CRISPR-Cas has emerged as the most practical toolkit to edit genomes. One of the most striking possibilities is to generate edited and non-transgenic plants. In this review, we present the working mechanism behind CRISPR-induced mutations and pinpoint the latest techniques developed, as well as its myriad of applications in agriculture. The enhancing scope of CRISPR ranges from introducing traits of agronomic interest – such as herbicide resistance, resistance/tolerance to biotic and abiotic stresses, and quality and durability of products – to accelerating plant breeding processes, including haploid induction, generating male-sterile lines, fixating hybrid vigor, and overcoming self-incompatibility. We also discuss regulatory issues surrounding edited plants and derived products around the world, challenges that must be overcome, and future prospects to harness all the potential of this amazing tool to guarantee the new crop production revolution.

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“…As an alternative to mtl -based haploid induction, centromeric histone H3 ( cenh3 ) heterozygous maize mutants can also induce haploids at levels up to 20%, so this system could also be combined with CRISPR/Cas9 to quickly produce edited plants in a wide array of germplasm and bypass transformation [ 113 ]. Haploid induction strategies assisted with seed-specific marker lines will help improve the efficiency of identifying CRISPR-edited plants with this technique to be able to rapidly identify haploids at the seed stage [ 114 , 115 ].…”

Section: Challenges and Emerging Technologies For Crispr/cas9-based Crop Improvementmentioning

confidence: 99%

Next Generation Cereal Crop Yield Enhancement: From Knowledge of Inflorescence Development to Practical Engineering by Genome Editing

Liu

Lindsay

Jackson

2021

IJMS

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Artificial domestication and improvement of the majority of crops began approximately 10,000 years ago, in different parts of the world, to achieve high productivity, good quality, and widespread adaptability. It was initiated from a phenotype-based selection by local farmers and developed to current biotechnology-based breeding to feed over 7 billion people. For most cereal crops, yield relates to grain production, which could be enhanced by increasing grain number and weight. Grain number is typically determined during inflorescence development. Many mutants and genes for inflorescence development have already been characterized in cereal crops. Therefore, optimization of such genes could fine-tune yield-related traits, such as grain number. With the rapidly advancing genome-editing technologies and understanding of yield-related traits, knowledge-driven breeding by design is becoming a reality. This review introduces knowledge about inflorescence yield-related traits in cereal crops, focusing on rice, maize, and wheat. Next, emerging genome-editing technologies and recent studies that apply this technology to engineer crop yield improvement by targeting inflorescence development are reviewed. These approaches promise to usher in a new era of breeding practice.

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Seeing is believing: a visualization toolbox to enhance selection efficiency in maize genome editing

Cited by 12 publications

References 10 publications

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

Genome editing: propelling the next generation of crop improvement

Next Generation Cereal Crop Yield Enhancement: From Knowledge of Inflorescence Development to Practical Engineering by Genome Editing

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