Seeing or believing in hyperplexed spatial proteomics via antibodies: New and old biases for an image-based technology
Maddalena M. Bolognesi,
Lorenzo Dall’Olio,
Amy Maerten
et al.
Abstract:Hyperplexed in-situ targeted proteomics via antibody immunodetection (i.e., >15 markers) is changing how we classify cells and tissues. Differently from other high-dimensional single-cell assays (flow cytometry, single-cell RNA sequencing), the human eye is a necessary component in multiple procedural steps: image segmentation, signal thresholding, antibody validation, and iconographic rendering. Established methods complement the human image evaluation, but may carry undisclosed biases in such a new contex… Show more
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