Segment-specific and common nucleotide sequences in the noncoding regions of influenza B virus genome RNAs.

Stoeckle, Mark Y.; Shaw, Michael; Choppin, Purnell W.

doi:10.1073/pnas.84.9.2703

Cited by 64 publications

(55 citation statements)

References 25 publications

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“…The second possibility is based on inspection of longer regions of complementarity found in naturally occurring cRNA molecules which suggest a more stable alignment (when adjacent sequences are included) by pairing, for example, position 11 (3' arm) with position 10' (5' arm) (Fig. 2a, middle;Stoeckle et at., 1987). Based on the relationship between vRNA and cRNA, this latter possibility is also predicted from the findings for vRNA model RNAs where position 11 (3' arm) paired with position 12' (5' arm) (Fodor et al, 1994(Fodor et al, , 1995.…”

Section: Crna Panhandle Investigationmentioning

confidence: 99%

In vitro transcription and polymerase binding studies of the termini of influenza A virus cRNA: evidence for a cRNA panhandle

Pritlove¹,

Fodor²,

Seong³

et al. 1995

Journal of General Virology

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Section: Crna Panhandle Investigationmentioning

confidence: 99%

In vitro transcription and polymerase binding studies of the termini of influenza A virus cRNA: evidence for a cRNA panhandle

Pritlove¹,

Fodor²,

Seong³

et al. 1995

Journal of General Virology

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“…The non coding regions contain cis-acting signals for the regulation of transcription and replication of viral RNA (35,36). Type-specific differences of the NCRs between members of the Orthomyxovirus family were analyzed in the last decade mostly by using in vitro transcription assays or in vivo reconstitution of vRNA-like templates encoding reporter genes such as chloramphenicol acetyltransferase (37)(38)(39)(40).…”

Section: Discussionmentioning

confidence: 99%

Rescue of influenza B virus from eight plasmids

Hoffmann

Mahmood

Yang

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

149

123

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Influenza B virus causes a significant amount of morbidity and mortality, yet the systems to produce high yield inactivated vaccines for these viruses have lagged behind the development of those for influenza A virus. We have established a plasmid-only reverse genetics system for the generation of recombinant influenza B virus that facilitates the generation of vaccine viruses without the need for time consuming coinfection and selection procedures currently required to produce reassortants. We cloned the eight viral cDNAs of influenza B͞Yamanashi͞166͞98, which yields relatively high titers in embryonated chicken eggs, between RNA polymerase I and RNA polymerase II transcription units. Virus was detected as early as 3 days after transfection of cocultured COS7 and Madin-Darby canine kidney cells and achieved levels of 10 6 -10 7 plaque-forming units per ml of cell supernatant 6 days after transfection. The full-length sequence of the recombinant virus after passage into embryonated chicken eggs was identical to that of the input plasmids. To improve the utility of the eight-plasmid system for generating 6 ؉ 2 reassortants from recently circulating influenza B strains, we optimized the reverse transcriptase-PCR for cloning of the hemagglutinin (HA) and neuraminidase (NA) segments. The six internal genes of B͞Yamanashi͞166͞98 were used as the backbone to generate 6 ؉ 2 reassortants including the HA and NA gene segments from B͞Victoria͞504͞2000, B͞Hong Kong͞ 330͞2001, and B͞Hawaii͞10͞2001. Our results demonstrate that the eight-plasmid system can be used for the generation of high yields of influenza B virus vaccines expressing current HA and NA glycoproteins from either of the two lineages of influenza B virus.

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“…Likewise the L-and medium (M) dsRNA segments in GH2 have identical sequences at the heteropolymer terminus (Tartaglia et al, 1986). Appearance of homologous terminal sequences at the respective ends of dsRNA segments is reminiscent of ss-and dsRNA viruses with segmented genomes (Nuss & Summers, 1984;Szekeres et al, 1985;Stoeckle et al, 1987).…”

confidence: 99%

Molecular Cloning of Hypovirulence-associated Double-stranded RNA in Endothia parasitica and Detection of Sequence-related Single-stranded RNA

Hiremath¹,

Nuss²,

Ghabrial³

et al. 1988

Journal of General Virology

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SUMMARYRecombinant plasmids containing cDNA copies of the dsRNAs present in one hypovirulent strain of Endothia (Cryphonectria)parasitica, EP713, were constructed and analysed by restriction endonuclease mapping and Southern hybridization. Overlapping inserts of four plasmids were found to represent most of the large dsRNA (L-dsRNA) sequences. Inserts which represented the two termini of the L-dsRNAs, designated 'homopolymer' and 'heteropolymer', were identified. These plasmids were used as probes in Northern hybridization experiments in an attempt to detect other RNAs having sequences related to those of the L-dsRNAs. No additional RNAs were detected that hybridized to plasmids representing the middle region of L-dsRNAs. However, plasmids representing the termini of L-dsRNAs hybridized to several RNAs in EP713 ranging in size from 300 to 1300 nucleotides. These RNAs were absent from EP 155, the isogenic virulent strain of E. parasitica. RNAs related to the homopolymer terminus were more abundant than those related to the heteropolymer terminus. All were sensitive to digestion by S1 nuclease but resistant to RNase III, indicating that they were single-stranded. Only those ssRNAs related to the homopolymer terminus of L-dsRNAs were retained on oligo(dT)-cellulose. Single-stranded M13 phage DNAs containing the insert of one of the plasmids, in each orientation, were used as probes in Northern hybridization experiments. Hybridization to the ssRNAs was observed with only one of these probes, indicating that the transcripts are derived from only one strand of the L-dsRNAs. These results establish the existence of a set of poly(A)-containing ssRNAs that are Y-coterminal with the homopolymer terminus of LdsRNAs and have the same polarity as the poly(A)-containing strand of L-dsRNAs.

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Segment-specific and common nucleotide sequences in the noncoding regions of influenza B virus genome RNAs.

Cited by 64 publications

References 25 publications

In vitro transcription and polymerase binding studies of the termini of influenza A virus cRNA: evidence for a cRNA panhandle

In vitro transcription and polymerase binding studies of the termini of influenza A virus cRNA: evidence for a cRNA panhandle

Rescue of influenza B virus from eight plasmids

Molecular Cloning of Hypovirulence-associated Double-stranded RNA in Endothia parasitica and Detection of Sequence-related Single-stranded RNA

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