Segmental bone regeneration using an rhBMP-2-loaded gelatin/nanohydroxyapatite/fibrin scaffold in a rabbit model

Liu, Yue; Yun, Lingsong; Xia, Tian; Geng, Changran; Zhao, Yanmei; Yang, Qiang; Yu, Sen; Xing, Guosheng; Zhang, Boxun

doi:10.1016/j.biomaterials.2009.08.003

Cited by 100 publications

(72 citation statements)

References 44 publications

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“…Moreover, due to short soaking time, the composite can be potentially used as a carrier for active substances (drugs, growth factors, etc.). This presumption is in agreement with the opinion that hybrid scaffolds are more profitable bone fillers and delivery systems than single component scaffolds [41]. However, the materials that reach the equilibrium of soaking several hours after the implantation may evoke undesired side effects because of the increased pressure within the implantation site, generated by swelling material.…”

Section: Soaking Propertiessupporting

confidence: 85%

Application of β-1,3-glucan in production of ceramics-based elastic composite for bone repair

et al. 2013

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Application of β-1,3-glucan in production of ceramics-based elastic composite for bone repair IntroductionHydroxyapatite (HAp), especially in a porous form, is appreciated as a bone filler due to its biocompatibility, bioactivity, osteoconductivity, minimal risk of appearance of allergic reactions, lack of carcinogenic properties and lack of sensitivity to sterilization processes [1][2][3]. There are numerous commercially available bone graft materials based on biologic and synthetic HAp, including ProOsteon ® (Interpore Cross International, USA), Endobon (BIOMET Orthopaedics, Switzerland), Cerapatite (Ceraver Osteal, France), Synatite (SBM, France) and others. HAp may serve not only as a bone filler but also as a carrier of active substances: antibiotics, chemiotherapeutics, growth factors, etc. [4][5][6][7][8][9]; it can also be used in composites, as a factor increasing their cytocompatibility, bioactivity, osteoconductivity, adhesion of coatings and compression modulus [10][11][12][13][14]. On the other hand, HAp application is often limited due to its relatively poor resorption and slow replacement by the host bone after implantation, substantially high Young modulus, and low fracture toughness [15,16], although these properties may be improved by addition of elasticity-increasing polymers. It is considered that polymer-ceramic composites reveal superior properties (at least in some aspects) over polymer and ceramic alone [17]. However, most polymers used for bone filler fabrication meet only some of the criteria for implantable materials.Bone replacing material such as ceramics or crushed bone offers an insufficient surgical handiness, especially when the orthopedic surgeon has to handle granules (in case of defects in maxillary bone) or scaffolds (rigid and non-adaptable to the implantation site). Some commercially available biomaterials contain the artificial or natural polymers serving as plasticizing agents and thus significantly reduce this inconvenience. Among them, EasyGraft TM (Degradable Solutions SA, Switzerland), Plexur M and P (Osteotech, USA) and Cerapatite-Collagen (Ceraver Osteal, France) can be listed. EasyGraft TM contains PLGA-coated β-TCP granules which hardens into a compact mass via partial and temporary PLGA dissolution by organic solvent; Plexur M and P are composed of cortical bone and resorbable artificial polymer; Cerapatite-Collagen, made from HAp granules and naturally-derived collagen fibers, becomes elastic after hydration with blood or saline. Bone or ceramic granules and powders may also be turned into a paste using collagen gels (e.g., Tecnoss ® Gel O; Tecnoss, Italy) or fibrin glue [18,19]. However, the natural polymeric compounds used in commercially available composites (collagen, fibrin glue) originate from animal sources; this can be considered as a limiting factor because of the increased risk of transmissible contamination (e.g. viruses) and undesirable immune responses. Thus, the necessity of careful purification of these animal polymers exacts the high price of fin...

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Section: Soaking Propertiessupporting

confidence: 85%

Application of β-1,3-glucan in production of ceramics-based elastic composite for bone repair

et al. 2013

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“…[34][35][36] Biological components including (but not limiting to) mesenchymal stem cells (MSCs) derived from various tissues such as bone marrow, 18 adipose tissue, 37 umbilical cord, 32 muscle, 38 skin, 39 tooth, 40 and osteoblasts from different regions [41][42][43] and also several biomolecules and growth factors like bone morphogenetic protein (BMP), 37 basic fibroblast growth factor (bFGF), 41 and vascular endothelial growth factor (VEGF) 44 can be used in combination with fibrin. Different properties of these adjacent materials led to different results both in vitro and in vivo.…”

mentioning

confidence: 99%

“…For instance, Zhao et al demonstrated that the incorporation of β-tricalcium phosphate nanoparticles in a fibrin glue significantly increased both proliferation and ALP expression of entrapped MSCs. 150 Therefore, fibrin composites, such as particulate allogenic bone/fibrin 151 and gelatin/nano HA/fibrin, 36 are preferred for cell delivery purposes over fibrin alone. The co-delivery of growth factors and cells is another attractive approach to enhance the efficiency of a fibrin/cell mixture.…”

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confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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“…Artificial devices containing beneficial functions, as is shown with the bone screws in our experiment, still need to be developed. Some experimental trials have been reported using PLLA screws or hydroxyapatite screws containing bioactive substances [14,24]. Additional studies are required to clarify the essential functions supplied by bone materials.…”

Section: Discussionmentioning

confidence: 99%

Bone Screws Have Advantages in Repair of Experimental Osteochondral Fragments

Kono

Mori

Uchio

2012

Clinical Orthopaedics &Amp; Related Research

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Background Cartilage defects are created on intraarticular osteochondral fragments at the entrance holes of fixation devices when these fragments are fixed to the original sites. Conventional fixation devices hinder repair of these defects and there is a latent risk of secondary osteoarthritis. We therefore developed a novel fixation device system consisting of bone screws made of cortical bone for osteochondral fragments to improve repair of these surface defects. Questions/purposes We asked whether bone screws had advantages over poly-L-lactic acid (PLLA) screws in terms of (1) gross assessment of the surface, (2) volume and histologic quality of the repair tissue, and (3) biomechanical assessment of the tissue stiffness.Methods We examined gross morphology, microCT, histology, and stiffness of the repaired tissue with PLLA (n = 32) and bone (n = 32) screws in a rabbit model of osteochondral fracture, compared with normal controls (n = 16). Results Gross morphology and histology revealed better quality with bone screws than with PLLA screws. Mean repaired volumes in microCT were 70.6% ± 14% with bone screws and 50.3% ± 15% with PLLA screws. Average stiffness values for PLLA screws, bone screws, and normal cartilage were 1.67 ± 0.54 N/mm, 2.63 ± 0.42 N/mm, and 3.15 ± 0.49 N/mm, respectively. Conclusions Our results show better repaired tissue was observed for quality and quantity when chondral fractures were treated with bone screws than when treated with PLLA screws. Clinical Relevance Bone screws made of cortical bone may have applications in clinical situations for the fixation of intraarticular osteochondral fragments.

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Segmental bone regeneration using an rhBMP-2-loaded gelatin/nanohydroxyapatite/fibrin scaffold in a rabbit model

Cited by 100 publications

References 44 publications

Application of β-1,3-glucan in production of ceramics-based elastic composite for bone repair

Application of β-1,3-glucan in production of ceramics-based elastic composite for bone repair

A review of fibrin and fibrin composites for bone tissue engineering

Bone Screws Have Advantages in Repair of Experimental Osteochondral Fragments

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