Segmental Cell Kinetics of the Human Anagen Hair: A DNA-Flow Cytometric Analysis

Cell Cycle Kinetics of Human Anagen Scalp Hair Bulbs in Thyroid Disorders Determined by DNA Flow Cytometry

“…Our DNA-FCM data derived from the hair bulb segment with highest mitotic activity [8,14], i.e. the bulbar part below the level of Auber, reveal statistically significant differences between proliferation index in hyper-and hypothyroid ism compared with healthy volunteers.…”

“…Former autoradiographic [ 14| and DNA-FCM studies [8] on cell kinetics of anagen hair bulbs have demon strated most of DNA-synthesizing cells to be matrix cells. Therefore, the increased proliferation index of anagen scalp hair bulbs in hyperthyroidism on the one hand and the decreased proliferation index in hypothyroidism on the other, indicate, assuming a constant S phase duration, a different proliferative activity of hair matrix cells.…”

“…Each prepared anagen hair bulb ( fig. 1) was horizontally cut at the widest diameter of the bulb (Auber's level) [method in detail: see 8). and dissected bulbar segments of each biopsy were collected separately.…”

“…FCM measurements were performed as described previously [8]. In brief, from the dissected bulbar parts of each biopsy a single-nuclei suspension was generated by adding 0.3 ml of 0.5% pepsin-I IC1 solu tion pH 1.5 (Scrva, Heidelberg, FRG) followed by vortexing of each suspension for 30 min at room temperature.…”