Segmental Difference in Epithelial and Mucosal Barrier Functions between the Jejunum and the Ileum in Cold Storage Small Bowel Grafts in the Rat

Abstract

The purpose of this study was to determine the differences in epithelial and mucosal barrier function between the jejunum and the ileum at transplantation. Rat small bowel was preserved in cold University of Wisconsin solution for 0, 12 or 24 hrs. Time-related segmental differences in the potential differences, at rest (rPD) and upon glucose stimulation (PDgs), and in the permeation rates of phenolsulfonphtalein (PSP) and polyethylene glycol 4000 (PEG) as non-absorbable markers were studied after a 20-min reoxygenation period following cold preservation. Time-related histologic damage during cold preservation was also evaluated. After 24 hrs of preservation, when the villi were denuded both in jejunum and ileum, the rPD (1.4 +/- 0.3 mV/cm2) and PDgs (1.4 +/- 0.3 mV/cm2) of the jejunum were significantly lower than those of the ileum (1.9 +/- 0.3 mV/cm2 and 2.1 +/- 0.3 mV/cm2, respectively). A difference in PEG permeation rates occurred after 24 hrs of preservation. The permeation rate of PSP in the jejunum was 10.5 +/- 1.0%, which was significantly higher than that in the ileum (8.6 +/- 1.0%) after 12 of preservation, when subepithelial edema occurred both in the jejunum and ileum. Our functional study demonstrated that, in the rat small bowel, the ileumis more resistant than the jejunum to cold preservation.

Segmental Difference of Mucosal Damage along the Length of a Mouse Small Intestine in an Ussing Chamber

“…Consistent with the present findings, a previous study has demonstrated that the ileum was more resistant to cold preservation than the jejunum (12). In contrast, the rat small intestine has exhibited no marked segmen tal difference in mucosal damage while in the Ussing chamber (5).…”

Garlic essential oil supplementation modulates colonic microbiota compositions and regulates immune response in weaned piglets

“…Previous researches reported that time-related damage of epithelial function occurred faster in the jejunum than in the ileum [ 35 ] and in a pig small transplantation experiment, which used cold ischemic and periods ranging from 0 to 12 h, estimated (based on the light microscopy) that the ileum is more resistant to preservation injury than jejunum [ 36 ]. Ulcerative colitis (UC) and Crohn's disease (CD) causes inflammation mostly in the ileum, colon, or both [ 37 ].…”