Segmentation And Modelling of Helanuclear Envelope

Karabağ, Cefa; Jones, Martin L.; Peddie, Christopher J.; Westor, A.E.; Collinson, Lucy; Reyes-Aldasoro, Constantino Carlos

doi:10.1109/isbi.2019.8759151

Cited by 2 publications

(2 citation statements)

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“…Details of the cell preparation have been published previously [77], but briefly, the data set consisted of EM images of HeLa cells, which were prepared and embedded in Durcupan resin following the method of the National Centre for Microscopy and Imaging Research (NCMIR) [78].…”

Section: Hela Cells Preparation and Acquisitionmentioning

confidence: 99%

Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning architectures

et al. 2020

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The quantitative study of cell morphology is of great importance as the structure and condition of cells and their structures can be related to conditions of health or disease. The first step towards that, is the accurate segmentation of cell structures. In this work, we compare five approaches, one traditional and four deep-learning, for the semantic segmentation of the nuclear envelope of cervical cancer cells commonly known as HeLa cells. Images of a HeLa cancer cell were semantically segmented with one traditional image-processing algorithm and four three deep learning architectures: VGG16, ResNet18, Inception-ResNet-v2, and U-Net. Three hundred slices, each 2000 × 2000 pixels, of a HeLa Cell were acquired with Serial Block Face Scanning Electron Microscopy. The first three deep learning architectures were pre-trained with ImageNet and then fine-tuned with transfer learning. The U-Net architecture was trained from scratch with 36, 000 training images and labels of size 128 × 128. The image-processing algorithm followed a pipeline of several traditional steps like edge detection, dilation and morphological operators. The algorithms were compared by measuring pixel-based segmentation accuracy and Jaccard index against a labelled ground truth. The results indicated a superior performance of the traditional algorithm (Accuracy = 99%, Jaccard = 93%) over the deep learning architectures:

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Section: Hela Cells Preparation and Acquisitionmentioning

confidence: 99%

Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning architectures

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Details of the cell preparation have been published previously [74], but briefly, the data set consisted of EM images of HeLa cells. HeLa cells were prepared and embedded in Durcupan resin following the method of the National Centre for Microscopy and Imaging Research (NCMIR) [75].…”

Section: Hela Cells Preparation and Acquisitionmentioning

confidence: 99%

Semantic Segmentation of HeLa Cells: An Objective Comparison between one Traditional Algorithm and Three Deep-Learning Architectures

Karabağ

Jones

Peddie

et al. 2020

Preprint

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In this work, images of a HeLa cancer cell were semantically segmented with one traditional image-processing algorithm and three deep learning architectures: VGG16, ResNet18 and Inception-ResNet-v2. Three hundred slices, each 2000 × 2000 pixels, of a HeLa Cell were acquired with Serial Block Face Scanning Electron Microscopy. The deep learning architectures were pre-trained with ImageNet and then fine-tuned with transfer learning. The image-processing algorithm followed a pipeline of several traditional steps like edge detection, dilation and morphological operators. The algorithms were compared by measuring pixel-based segmentation accuracy and Jaccard index against a labelled ground truth. The results indicated a superior performance of the traditional algorithm (Accuracy = 99%, Jaccard = 93%) over the deep learning architectures: VGG16 (93%, 90%), ResNet18 (94%, 88%), Inception-ResNet-v2 (94%, 89%).

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Segmentation And Modelling of Helanuclear Envelope

Cited by 2 publications

References 15 publications

Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning architectures

Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning architectures

Semantic Segmentation of HeLa Cells: An Objective Comparison between one Traditional Algorithm and Three Deep-Learning Architectures

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