Segregation distortion caused by weak hybrid necrosis in recombinant inbred lines of common wheat

Abstract

Segregation distortion of molecular markers is closely related to hybrid incompatibility in progeny from intraspecific crosses. Recent reports in higher plants have demonstrated that hybrid sterility results in segregation distortion at the causal gene regions in progeny of intraspecific crosses. Ne1 and Ne2 complementary loci are known to control hybrid necrosis in intraspecific crosses of common wheat cultivars. Here, we examine the effect of a weak necrosis allele Ne1(w) on the segregation ratio of molecular markers in recombinant inbred lines (RILs) of common wheat. Some RILs showed accelerated cell death in the leaves at the heading stage due to the epistatic interaction between two quantitative trait loci (QTL) on chromosomes 5B and 2B. Chromosomal localization of these QTL corresponding to Ne1(w) and Ne2 showed distorted segregation ratios of assigned markers having oppositely biased direction. Although the Ne1(w) and Ne2 interaction had no obvious effect on seed fertility, Ne1(w) reduced completion of grain development under the Ne2-homozygous background. This reduction might be one of causes that induces segregation distortion in the 5B and 2B chromosomal regions of RILs. The present study demonstrated that weak hybrid necrosis has limited phenotypic effects; it causes segregation distortion in progeny from intraspecific crosses.

Development of two major resources for pea genomics: the GenoPea 13.2K SNP Array and a high‐density, high‐resolution consensus genetic map

“…It is thus important to gain knowledge about the genetics of segregation distortion in pea. Excluding environmental factors that occur during the course of population development and genotyping errors, different factors could influence the rate and chromosomal location of segregation aberration including: gametophytic and/or sterility gene loci (Castro et al ., ; Baumbach et al ., ), loci involved in the process of meiotic division or early stages of reproductive cell development, and epistasis between incompatible loci (Takumi et al ., ). High‐density molecular linkage maps obtained from various populations within a species provide a great opportunity to survey the entire genome for segregation distortion loci.…”

Identification and Characterization of Segregation Distortion Loci on Cotton Chromosome 18

“…With the development of molecular markers, SD has been widely reported in several plant species, particularly in interspecific crosses for genetic mapping, such as rice (Shanmugavadivel et al, 2013), wheat (Takumi et al, 2013; Adamski et al, 2014), chickpea (Ravikumar et al, 2013), Arabidopsis (Leppala et al, 2013), coffee (Gartner et al, 2013), soybean (Baumbach et al, 2012), potato (Manrique-Carpintero et al, 2016), Brassica rapa (Kitashiba et al, 2016), and Populus deltoids (Zhou et al, 2015). SD has previously been reported in interspecific populations of cotton (Guo et al, 2007; Blenda et al, 2012; Byers et al, 2012; Liang et al, 2012; Yu et al, 2013; Diouf et al, 2014; Zhang et al, 2014; Li et al, 2016), intraspecific populations of G. barbadense (Wang et al, 2013), intraspecific populations of G. hirsutum (Lin et al, 2009; Zhang et al, 2012, 2016; Ning et al, 2014), and intraspecific populations of G. arboreum (Li et al, 2007); however, the genetic mechanism of SD in cotton remains unclear.…”

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

“…Reactive oxygen species generation and necrotic cell death were effectively inhibited by ZnCl 2 treatment in types I, III and Electronic supplementary material The online version of this article (doi:10.1007/s11103-015-0338-6) contains supplementary material, which is available to authorized users. on chromosome arms 5BL and 2BS, and the induction of necrotic cell death by Ne1-Ne2 is called type I hybrid necrosis (Chu et al 2006;Takumi et al 2013;Tsunewaki 1960Tsunewaki , 1992. Type I necrosis functions as a reproductive barrier contributing to intraspecific differentiation mainly between European and American varieties and middle Asian and African varieties in common wheat (Pukhalskiy et al 2000).…”