The Matano fault is one of the fast-slipping faults in Indonesia with a slip rate of ~20 mm/yr, accommodating the motion between the Pacific and Australian plates. The fault has remained unruptured at least for the past two centuries. Recent paleoseismological study suggested that the Matano fault has accumulated enough slip for the next surface-rupturing earthquakes. However, studies on the structures, seismicity, and deformation rate of the Matano fault are very scarce. This study characterizes the fault’s segmentation, evaluates the modern seismicity, and calculates the deformation rate of the Matano fault. The segmentation model of the Matano fault comprises eight major geometric segments, separated by structural discontinuities. Analysis of modern seismicity shows the high a- and b-value on the Matano fault, suggesting the high tectonic activity. Result of InSAR modeling also shows the rapid deformation on the Matano fault, reaching 39 mm/yr. Integrating our results suggest that the Matano fault can be divided into two major portions, separated by Lake Matano, that could rupture independently and produce Mw ≥7 earthquakes, possibly similar to the 2018 Mw 7.5 Palu earthquake. This study highlights the importance of comprehensive approaches for better understanding the active fault behaviors.