independent variables (inoculums age, volume and concentration of sulphuric acid) and their interactions significantly influenced the hydrogen production. Optimum conditions optimized during the study were inoculums age, 21 h, inoculums volume, 50 ml/l and concentrations of sulphuric acid, 1.25%. Maximum bio-hydrogen production obtained with these optimum conditions was 705 ml. Results show that RSM (CCD) was a useful statistical tool for optimization of the key factors influencing the biohydrogen production.Conflict of interest: No conflict of interest was declared.Author's contribution: Veena Thakur and Mona Tandon were involved in modelling and designing of the experiment. Final manuscript drafting and compiling was done by S. K. Jadhav. Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge.