Seizure logging: A new approach to synchronized cable-free EEG and video recordings of seizure activity in mice

Etholm, Lars; Arabadzisz, Dimitrula; Lipp, Hans‐Peter; Heggelund, Paul

doi:10.1016/j.jneumeth.2010.08.003

Cited by 20 publications

(12 citation statements)

References 15 publications

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“…The acute effects of these cannabinoids on sleep were assessed using epidural EEG recordings through wireless microchip technology, which has previously only been applied to mice [18, 21]. This novel technology produced results similar to previous work on other CB1 receptor agonists using tethered EEG recordings [1]; we report alterations in sleep-wake architecture by enhancing NREM sleep mainly at the expense of wakefulness following cannabinoid administration.…”

Section: Discussionmentioning

confidence: 57%

A Pilot Study into the Effects of the CB1 Cannabinoid Receptor Agonist WIN55,212-2 or the Antagonist/Inverse Agonist AM251 on Sleep in Rats

et al. 2011

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The plant cannabinoid Δ9-tetrahydrocannabinol and the endocannabinoid anandamide increase the amount of sleep via a CB1 receptor mediated mechanism. Here, we explored the use of a novel electroencephalogram (EEG) recording device based on wireless EEG microchip technology (Neurologger) in freely-moving rats, and its utility in experiments of cannabinoids-induced alterations of EEG/vigilance stages. EEG was recorded through epidural electrodes placed above pre-frontal and parietal cortex (overlaying the dorsal hippocampus). As cannabinoids, we acutely administered the full synthetic CB1 receptor agonist, WIN55,212-2 (1 mg/kg), and the antagonist/inverse agonist, AM251 (2 mg/kg), either alone or together through the intraperitoneal route. WIN55,212-2 increased the total amount of NREM sleep and the length of each NREM bout, but this was unlikely due to CB1 receptor activation since it was not prevented by AM251. However, WIN55,212-2 also lowered overall EEG spectral power especially in theta and alpha frequency bands during wakefulness and NREM sleep, and this effect was reversed by AM251. The antagonist/inverse agonist caused no sleep alterations by itself and moderately increased spectral power in Theta, alpha and beta frequency bands during NREM sleep when administered on its own. Implications of endocannabinoid modulation of the sleep-wake cycle and its possible interactions with other transmitter systems are considered.

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Section: Discussionmentioning

confidence: 57%

A Pilot Study into the Effects of the CB1 Cannabinoid Receptor Agonist WIN55,212-2 or the Antagonist/Inverse Agonist AM251 on Sleep in Rats

et al. 2011

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“…LFPs were recorded from the mPFC and NAcc of behaving females using a battery-powered Neurologger 35 chip (1-GB model, New Behavior AG). The Neurologger has 8 channels (4 neural data, 2 reference, 1 accelerometer, 1 infrared synchronization) and samples up to 500 Hz.…”

Section: Methodsmentioning

confidence: 99%

Dynamic corticostriatal activity biases social bonding in monogamous female prairie voles

Amadei

Johnson

Kwon

et al. 2017

Nature

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Summary paragraph Adult pair bonding involves dramatic changes in the perception and valuation of another individual1. One key change is that partners come to reliably activate the brain's reward system2-6, though the precise neural mechanisms by which partners become rewarding during sociosexual interactions leading to a bond remain unclear. Using a prairie vole model of social bonding7, we show how a functional circuit from medial prefrontal cortex (mPFC) to nucleus accumbens (NAcc) is dynamically modulated to enhance females' affiliative behavior towards a partner. Individual variation in the strength of this functional connectivity, particularly after the first mating encounter, predicts how quickly animals begin affiliative huddling with their partner. Rhythmically activating this circuit in a social context without mating biases later preference towards a partner, indicating that this circuit's activity is not just correlated with how quickly animals become affiliative but causally accelerates it. These results provide the first dynamic view of corticostriatal activity during bond formation, revealing how social interactions can recruit brain reward systems to drive changes in affiliative behavior.

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“…Typically, screws are used as electrodes or anchors for wires serving as electrodes and there are two different approaches for affixing the screws. One features drilling holes in the skull and inserting screws, which requires careful precision to avoid drilling or inserting the screws too deep and causing brain damage (Lapray et al, 2008 ; Armstrong et al, 2013 ), while the other avoids drilling and instead affixes the screws to the surface of the skull with cyanoacrylate glue (Etholm et al, 2010 ), which can result in poor contact if there is excessive glue between the skull and screw. After utilizing both approaches to record EEG on mice with a wireless recording system, with a lack of consistent successful implantation and adequate signal conduction, our lab decided to investigate alternative approaches and have developed procedures that result in consistent successful surgeries and recordings, with a robust implant that is minimally invasive and produces recordings for a month or more after implantation.…”

Section: Discussionmentioning

confidence: 99%

Low Cost Electrode Assembly for EEG Recordings in Mice

et al. 2017

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Wireless electroencephalography (EEG) of small animal subjects typically utilizes miniaturized EEG devices which require a robust recording and electrode assembly that remains in place while also being well-tolerated by the animal so as not to impair the ability of the animal to perform normal living activities or experimental tasks. We developed simple and fast electrode assembly and method of electrode implantation using electrode wires and wire-wrap technology that provides both higher survival and success rates in obtaining recordings from the electrodes than methods using screws as electrodes. The new wire method results in a 51% improvement in the number of electrodes that successfully record EEG signal. Also, the electrode assembly remains affixed and provides EEG signal for at least a month after implantation. Screws often serve as recording electrodes, which require either drilling holes into the skull to insert screws or affixing screws to the surface of the skull with adhesive. Drilling holes large enough to insert screws can be invasive and damaging to brain tissue, using adhesives may interfere with conductance and result in a poor signal, and soldering screws to wire leads results in fragile connections. The methods presented in this article provide a robust implant that is minimally invasive and has a significantly higher success rate of electrode implantation. In addition, the implant remains affixed and produces good recordings for over a month, while using economical, easily obtained materials and skills readily available in most animal research laboratories.

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Seizure logging: A new approach to synchronized cable-free EEG and video recordings of seizure activity in mice

Cited by 20 publications

References 15 publications

A Pilot Study into the Effects of the CB1 Cannabinoid Receptor Agonist WIN55,212-2 or the Antagonist/Inverse Agonist AM251 on Sleep in Rats

A Pilot Study into the Effects of the CB1 Cannabinoid Receptor Agonist WIN55,212-2 or the Antagonist/Inverse Agonist AM251 on Sleep in Rats

Dynamic corticostriatal activity biases social bonding in monogamous female prairie voles

Low Cost Electrode Assembly for EEG Recordings in Mice

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