Selbstreplikation eines Peptids unter Ionenkontrolle

In 1997, Wang and Sutherland reported a case of selfreplication in a Diels-Alder reaction.[1] This system is remarkable for at least two reasons: it constituted the first example of nearly exponential growth and it had the potential to exhibit two kinds of self-replication, homochiral autocatalysis and heterochiral cross-catalysis, because a chiral diene was employed. Unfortunately, the authors could not complete a stereochemical analysis of their system. We report herein mechanistic and stereochemical studies of variants of the Wang-Sutherland replicator. We show that only one of four potential diastereomers is formed within our detection limits and that this diastereomer is involved in both autocatalytic and cross-catalytic channels. A computational study suggests that the system can be classified as an example of a nearby

show abstract

Autonomous Fueled Mechanical Replication of Nucleic Acid Templates for the Amplified Optical Detection of DNA

Weizmann

Cheglakov

Pavlov

et al. 2006

Angewandte Chemie

View full text Add to dashboard Cite

The self-replication of molecular or macromolecular units has attracted substantial research efforts over the past fifteen years, and the relevance of these systems to prebiotic chemical evolution and optimal synthetic machines has been discussed.[1] The different replication systems that have been developed include nucleotide-based oligomers, [2][3][4][5][6] peptides, [7][8][9][10] molecular conjugates, [11,12] micelles, [13] and vesicles.[14] The application of self-replicating systems is scarce, and only chemical amplification in synthesis [15,16] or the preparation of nanoparticles in self-reproducing micelles [17] were demonstrated. Amplification is a central aspect in bioanalytical science, and enzyme conjugates, [18] DNAzymes, [19] and, more recently, nanoparticles [20] have been widely used as amplifying labels for biorecognition events. The polymerase chain reaction (PCR) is the standard method for the chemical amplification of nucleic acids.The information encoded in DNA sequences and structures has also been employed to develop nanomachines [21,22] such as unidirectional walkers [23] and gears.[24] Herein, we report on the development of an enzyme/DNA scission machine that is triggered by the hybridization of a pretailored DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary dye/quencher-functionalized nucleic acid, and upon operation, it replicates the machinery cutter. The scission of the fuel yields a fluorescent waste product that provides the optical readout signal for the replicating machine. Herein, we describe the analysis of one of the Tay-Sachs genetic disorder mutants. The method reveals a new concept that could substitute PCR amplification and highlights the use of self-replication in amplified DNA sensing.The operation of the replication machine is discussed with regards to, first, the formation of the scission machine and its operation in the presence of the dye/quencher nucleic acid fuel (Figure 1) and, second, the triggering of the DNA scission

show abstract

Selbstreplikation eines Peptids unter Ionenkontrolle

Cited by 23 publications

References 39 publications

Replicators: Components for Systems Chemistry

Replicators: Components for Systems Chemistry

Systems Chemistry: Kinetic and Computational Analysis of a Nearly Exponential Organic Replicator

Autonomous Fueled Mechanical Replication of Nucleic Acid Templates for the Amplified Optical Detection of DNA

Contact Info

Product

Resources

About