Select the best: novel biocatalysts for industrial applications

Konarzycka-Bessler, Monika; Jaeger, Karl‐Erich

doi:10.1016/j.tibtech.2006.04.004

Cited by 18 publications

(12 citation statements)

References 16 publications

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“…They demonstrated that it was possible to modify the system using alternative promoters and reporter genes. Modifications to this system, such as application of alternative transcriptional regulators, could expand the range of biocatalysts detected [Konarzycka-Bessler and Jaeger, 2006].…”

Section: Product-based Detection Systemsmentioning

confidence: 99%

Advances in Recovery of Novel Biocatalysts from Metagenomes

et al. 2008

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Metagenomics has accelerated the process of discovery of novel biocatalysts by enabling scientists to tap directly into the entire diversity of enzymes held within natural microbial populations. Their characterization has revealed a great deal of valuable information about enzymatic activity in terms of factors which influence their stability and activity under a wide range of conditions. Many of the biocatalysts have particular properties making them suitable for biotechnological applications. A diverse array of strategies has been developed to optimize each step of the process of generating and screening metagenomic libraries for novel biocatalysts. This review covers the diversity of metagenome-derived enzymes characterized to date, and the strategies currently being developed to optimize discovery of novel metagenomic biocatalysts.

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Section: Product-based Detection Systemsmentioning

confidence: 99%

Advances in Recovery of Novel Biocatalysts from Metagenomes

et al. 2008

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“…Thus compared to screening, the selection method allows for analysing much larger libraries, namely ~ 10 9 versus ~ 10 5 . It should be mentioned that more recent screening techniques such as cells‐in‐droplet screens coupled to FACS are also used to screen relatively big libraries (~ 10 9 ).…”

Section: General Overview Of In Vivo Screening and Selection Strategiesmentioning

confidence: 99%

Reporter‐based screening and selection of enzymes

2013

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The biotech industry is continuously seeking for new or improved biocatalysts. The success of these efforts is often hampered by the lack of an efficient screening assay. Thus, to be able to extend the number of enzymes available for industrial applications, high-throughput screening and selection methods are required. In the last few years an impressive range of screening and selection strategies has been developed. In this review, we will mainly focus on in vivo reporter systems in which the activity of a reporter is controlled by the activity of an enzyme of interest. Different mechanisms can be distinguished: (a) binding of the product of the enzymatic reaction to a transcriptional regulator and thereby turning on transcription of the reporter; (b) direct modification of a transcriptional regulator by the enzyme resulting in expression of the reporter; (c) binding of the product to a regulatory riboswitch or ribozyme, resulting in translation of the reporter; and (d) direct modification of the reporter by the enzyme, altering the reporter's activity. The choice for either a selection or a screening strategy depends on the type of reporter, e.g. providing antibiotic resistance (selection) or transmitting a fluorescent signal (screening). Although developing the specificity of each of these reporter-based selection or screening systems towards a certain enzymatic reaction is not yet straightforward, their adjustable modular design appears to be a promise for general applicability in the near future.

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“…Multi-well plates have been intensively used for high throughput screening of enzyme activity, in assays based in the production (depletion) of a chromogenic or fluorogenic product (substrate), allowing for the simultaneous evaluation of up to 10 6 different variants [29,44]. Screening can be performed in liquid media or in solid media [45].…”

Section: Miniature Systemsmentioning

confidence: 99%

Miniaturization in Biocatalysis

Fernandes

2010

IJMS

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The use of biocatalysts for the production of both consumer goods and building blocks for chemical synthesis is consistently gaining relevance. A significant contribution for recent advances towards further implementation of enzymes and whole cells is related to the developments in miniature reactor technology and insights into flow behavior. Due to the high level of parallelization and reduced requirements of chemicals, intensive screening of biocatalysts and process variables has become more feasible and reproducibility of the bioconversion processes has been substantially improved. The present work aims to provide an overview of the applications of miniaturized reactors in bioconversion processes, considering multi-well plates and microfluidic devices, update information on the engineering characterization of the hardware used, and present perspective developments in this area of research.

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Select the best: novel biocatalysts for industrial applications

Cited by 18 publications

References 16 publications

Advances in Recovery of Novel Biocatalysts from Metagenomes

Advances in Recovery of Novel Biocatalysts from Metagenomes

Reporter‐based screening and selection of enzymes

Miniaturization in Biocatalysis

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