Selected Activities of Citrus Maxima Merr. Fruits on Human Endothelial Cells: Enhancing Cell Migration and Delaying Cellular Aging

Buachan, Paiwan; Chularojmontri, Linda; Wattanapitayakul, Suvara K.

doi:10.3390/nu6041618

Cited by 37 publications

(23 citation statements)

References 43 publications

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“…Numerous studies have shown that replicative senescence can be viewed as a result of a constant moderate stress such as stimuli from reactive oxygen species (ROS) to the cells [22]. Continuous cultivation of HUVEC cells could promote endogenous formation of ROS [23,24]. In this study, we observed gradually decreased levels of several antioxidant molecules (glutathione, creatine and fumarate).…”

Section: Promoted Oxidative Stress During Cellular Replicative Senescmentioning

confidence: 53%

NMR-based metabonomic analysis of HUVEC cells during replicative senescence

Lin

Jiang

et al. 2020

Aging

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Cellular senescence is a physiological process reacting to stimuli, in which cells enter a state of irreversible growth arrest in response to adverse consequences associated with metabolic disorders. Molecular mechanisms underlying the progression of cellular senescence remain unclear. Here, we established a replicative senescence model of human umbilical vein endothelial cells (HUVEC) from passage 3 (P3) to 18 (P18), and performed biochemical characterizations and NMR-based metabolomic analyses. The cellular senescence degree advanced as the cells were sequentially passaged in vitro, and cellular metabolic profiles were gradually altered. Totally, 8, 16, 21 and 19 significant metabolites were primarily changed in the P6, P10, P14 and P18 cells compared with the P3 cells, respectively. These metabolites were mainly involved in 14 significantly altered metabolic pathways. Furthermore, we observed taurine retarded oxidative damage resulting from senescence. In the case of energy deficiency, HUVECs metabolized neutral amino acids to replenish energy, thus increased glutamine, aspartate and asparagine at the early stages of cellular senescence but decreased them at the later stages. Our results indicate that cellular replicative senescence is closely associated with promoted oxidative stress, impaired energy metabolism and blocked protein synthesis. This work may provide mechanistic understanding of the progression of cellular senescence.

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Section: Promoted Oxidative Stress During Cellular Replicative Senescmentioning

confidence: 53%

NMR-based metabonomic analysis of HUVEC cells during replicative senescence

Lin

Jiang

et al. 2020

Aging

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“…Cellular senescence is a major cause of aging. It is characterized by numerous biochemical changes, such as the increased activity of β-galactosidase [ 25 ]. It is well known that the exposure of H 2 O 2 induces higher β-galactosidase activity that allows an in vitro identification of senescent cells [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study

et al. 2020

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Gallic acid (GA), a plant-derived ubiquitous secondary polyphenol metabolite, can be a useful dietary supplement. This in vitro study’s primary purpose was to assess the anti-aging properties of GA using rat embryonic fibroblast (REF) cells, antidiabetic effects via pancreatic islet cells, and finally, elucidating the molecular mechanisms of this natural compound. REF and islet cells were isolated from fetuses and pancreas of rats, respectively. Then, several senescence-associated molecular and biochemical parameters, along with antidiabetic markers, were investigated. GA caused a significant decrease in the β-galactosidase activity and reduced inflammatory cytokines and oxidative stress markers in REF cells. GA reduced the G0/G1 phase in senescent REF cells that led cells to G2/M. Besides, GA improved the function of the β cells. Flow cytometry and spectrophotometric analysis showed that it reduces apoptosis via inhibiting caspase-9 activity. Taken together, based on the present findings, this polyphenol metabolite at low doses regulates different pathways of senescence and diabetes through its antioxidative stress potential and modulation of mitochondrial complexes activities.

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“…In this context and in the framework of the ageing process, citrus crops are one of the most important in the world, as producers of high vitamin C and polyphenolic content, and the fruits are among the most valuable functional diets, highly consumed globally in the form of fresh as well as processed juices and shown to lower oxidative stress-related diseases and ageing [6]. Furthermore, an extract of pummelo ( Citrus maxima ) was able to attenuate increased ROS levels in aged cells [7], while epidemiological studies reveal a strong correlation between high levels of citrus fruit consumption and low incidence of age-related diseases such as cardiovascular diseases and cancer [8]. Besides, citrus fruits showed cytoprotective action which is enhanced by the presence of antioxidants including vitamin C and flavonoids [9].…”

Section: Introductionmentioning

confidence: 99%

HPLC-UV-MS Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile

et al. 2014

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Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7-9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MS n fragmentations. Ascorbic acid content was higher in the pulps of OPEN ACCESSMolecules 2014, 19 17401 the varieties Genova and Sutil (60.13 ± 1.28 and 56.53 ± 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 ± 0.81 and 25.58 ± 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 ± 1.23 µg/mL in the DPPH assay and 120.63 ± 2.45 µM trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products.

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Selected Activities of Citrus Maxima Merr. Fruits on Human Endothelial Cells: Enhancing Cell Migration and Delaying Cellular Aging

Cited by 37 publications

References 43 publications

NMR-based metabonomic analysis of HUVEC cells during replicative senescence

NMR-based metabonomic analysis of HUVEC cells during replicative senescence

Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study

HPLC-UV-MS Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile

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