Selected aspects of apoptosis in psoriasis

Myśliwiec, Hanna; Baran, Anna; Flisiak, Iwona

doi:10.5114/dr.2017.66223

Cited by 4 publications

(6 citation statements)

References 47 publications

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“…However, there are no significant differences in Bcl-2 expression in the epidermis or Bcl-2 levels in the serum of patients with psoriasis compared to healthy individuals. Indeed, the presence of proteins from the Bcl-2 family in the epidermis may not be the sole determinant, but rather their relative ratios and the predominance of one over the others likely play a meaningful role [16]. In psoriasis, keratinocytes produce various chemokines, such as CCL20 and CXCL10, which recruit Th1/Tc1 and Th17 cells [17].…”

Section: Discussionmentioning

confidence: 99%

The Unknown Role of Periostin in Psoriatic Epidermal Hyperplasia

Wojciechowska,

Ścibior,

Betyna-Białek

et al. 2023

IJMS

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Psoriasis is an inflammatory skin disease that affects 1–2% of the general population. The pathomechanism is based on type 1 immunological reactions. Hyperplasia of the epidermis in psoriasis is a result of disrupted epidermal architecture due to increased synthesis and expression of extracellular matrix proteins. In our study, we analyzed the involvement of periostin (POSTN) in the pathogenesis of psoriasis, as one of the extracellular matrix proteins belonging to the fasciclin family. The study group consisted of 70 patients with psoriasis, while the control group comprised 30 healthy individuals. The serum concentrations of POSTN, Il-6, Il-17, Il-22, TNF-α and IFN-γ were measured in all participants. The severity of psoriasis was determined using the PASI (Psoriasis Area and Severity Index) score. The presence of POSTN in biopsy samples of 50 patients was assessed using the direct immunofluorescence method. The results were subjected to statistical analysis. The serum concentrations of POSTN, Il-6, Il-17, Il-22, TNF-α and IFN-γ in the study group are significantly higher than in the control group. Positive correlation has been demonstrated between the PASI score and the investigated cytokines, but not with POSTN. There was no statistically significant correlation between the POSTN level and the cytokines levels. POSTN deposits were localized in the epidermis in 66% of patients with psoriasis. The role of POSTN in the pathogenesis of psoriasis remains unclear. The mechanisms inducing the synthesis and expression of POSTN in psoriatic skin are not yet fully understood. Further research is needed to enhance our understanding of the mechanism underlying epidermal hyperplasia in psoriasis.

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Section: Discussionmentioning

confidence: 99%

The Unknown Role of Periostin in Psoriatic Epidermal Hyperplasia

Wojciechowska,

Ścibior,

Betyna-Białek

et al. 2023

IJMS

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“…One of the critical processes whose disorder is observed in the course of psoriasis is the loss of the balance between the process of apoptosis and cell proliferation [3,24]. As part of this work, we decided to evaluate the effect of adalimumab therapy on the gene expression profile that is involved in apoptosis in patients with psoriatic arthritis and in healthy volunteers.…”

Section: Discussionmentioning

confidence: 99%

“…The anti-apoptotic Bcl-2 family of proteins regulates this type of path. Bid, Bad, Bak, and Bax are examples of pro-apoptotic proteins [3].…”

Section: Introductionmentioning

confidence: 99%

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mRNA level of ROCK1, RHOA, and LIMK2 as genes associated with apoptosis in evaluation of effectiveness of adalimumab treatment

Krawczyk

Strzałka‐Mrozik

Grabarek³

et al. 2020

Pharmacol. Rep

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Background Psoriasis is a multifactorial autoimmune disease, which underlies the abnormalities of the apoptotic process. In cases of psoriasis and psoriatic arthritis, biological treatment is used. This study aimed to determine any changes in the expression of the genes associated with apoptosis in patients with psoriatic arthritis treated with adalimumab and to assess any phenotypic modifications based on changes in dermatological indexes. Methods The study included 20 patients with psoriatic arthritis treated biologically and 20 healthy volunteers. The research material consisted of peripheral blood mononuclear cells (PBMCs) from which the total RNA was isolated. Changes in the gene expression were determined using oligonucleotide microarrays and RT-qPCR. The clinical condition was assessed based on selected indicators: PASI, BSA [%], DAS28, and DLQI, which were determined every 3 months. Results There were changes in the expression of genes associated with apoptosis. Significant differences were found for ROCK1, RhoA, and LIMK2 expression profiles in PBMCs. At the initial stage of treatment, a decrease in the PASI and BSA rates was observed. At the later stages, the values of these indicators increased once again. There were correlations between the changes in these genes' expression and the dermatological markers. Conclusion Adalimumab influences the expression of genes related to apoptosis and the values of dermatological indicators of patients. Changes in the expression level of genes associated with apoptosis suggest that ROCK1, RhoA, and LIMK2 may be genes that can potentially be indicators of treatment effectiveness and lack of response to biological treatment.

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“…Apoptosis is an active process whose induction begins after the action of the appropriate stimulus. This process is regulated by many factors, including the BCL‐2 family of proteins (Myśliwiec, Baran, & Flisiak, ). Antiapoptotic proteins belong to this group of proteins, an example of which is the Bcl‐xL protein that is encoded by the BCL2L1 gene.…”

Section: Introductionmentioning

confidence: 99%

Adalimumab changes the expression profile of selected BCL‐2 family genes

Krawczyk

Strzałka‐Mrozik

Wcisło‐Dziadecka

et al. 2020

Dermatologic Therapy

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Biological drugs are an alternative to treatment of psoriasis and psoriatic arthritis. Adalimumab is a representative of the anti‐TNF group. The underlying of this disease is a cellular homeostasis disorder—apoptosis. Many proteins are involved in the apoptosis induction pathways, including those from the BCL‐2 family. The aim of the study was to perform a transcriptional analysis of the genes coding selected proteins from the BCL‐2 family in patients treated with adalimumab therapy, and to determine the direction of these changes. The test materials were peripheral blood mononuclear cells. The cells were obtained from 20 patients with psoriatic arthritis who were being treated with adalimumab (study group) and 20 healthy volunteers (control). The gene expression profile was determined using the real‐time quantitative reverse transcription polymerase chain reaction technique. Statistically significant changes were observed in the expression level of the BNIP3, BNIP3L, and BCL2L1 genes (p < .05) during a 24‐month observation of therapy. We indicated that adalimumab therapy has an impact on the expression of the analyzed genes, which may constitute a new class of molecular markers for assessing the effectiveness of a therapy. It appears that the BNIP3L gene could be used as a potential diagnostic marker of psoriasis.

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Selected aspects of apoptosis in psoriasis

Cited by 4 publications

References 47 publications

The Unknown Role of Periostin in Psoriatic Epidermal Hyperplasia

The Unknown Role of Periostin in Psoriatic Epidermal Hyperplasia

mRNA level of ROCK1, RHOA, and LIMK2 as genes associated with apoptosis in evaluation of effectiveness of adalimumab treatment

Adalimumab changes the expression profile of selected BCL‐2 family genes

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