Selected plasma biochemistry parameters in gilthead seabream (Sparus aurata) juveniles

Abstract

The aim of this study was to assess plasma biochemistry parameters with the potential of being used as indicators of the nutritional status for healthy gilthead seabream juveniles. Triplicate groups of 18 seabream (body weight of 58 g) were kept unfed for 24 h, 7 or 14 days. Nine fish per treatment were then sampled randomly for blood collection and the following parameters analyzed in the plasma using standard clinical methods: glucose; protein; triglycerides; cholesterol; calcium; magnesium; inorganic phosphorus; alkaline phosphatase (ALP); aspartate aminotransferase (AST); lactate dehydrogenase (LDH); gamma-glutamyl transferase (GGT); creatine phosphokinase (CPK); and lipase. Biochemical parameters showed lower variability among individuals than did enzymatic parameters. Plasma glucose, protein, cholesterol, calcium and inorganic phosphorus levels were inversely related to the duration of starvation. On the contrary, plasma triglycerides decreased significantly during the first week of starvation and remained stable in the second week. Plasma ALP, AST and LDH decreased significantly after 1 week of starvation and then remained constant. In healthy seabream juveniles, plasma glucose, protein, cholesterol, calcium and inorganic phosphorus are responsive to starvation and may be useful indicators of the nutritional status of the animals. Indicative baseline reference values for gilthead seabream juveniles starved for 24 h and held at optimum temperature are: protein, 3.7-4.9 g dl À1 ; cholesterol, 341-407 mg dl À1 ; calcium, 13.1-8.0 mg dl À1 ; and inorganic phosphorus, 10-14.2 mg dl À1 . Plasma triglycerides, along with plasma enzyme activities, may be useful as indicators of short term starvation. For these parameters baseline values after 1 week of starvation were: triglycerides: 138-230 mg dl À1 ; ALP: 58-125 U L À1 ; AST: 15-127 U L À1 ; and LDH 61-677 U L À1 . Plasma glucose is only responsive to longer starvation periods, remaining relatively stable during the first week of starvation, and ranging from 59 to 196 mg dl À1 . U.S.

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“…AST values in our study were comparable with Peres et al (2013). Although Peres et al (2013) reported cholesterol level in juvenile sea bream (left hungry for 24 hours) as 361.6 mg/dl (341-407), the highest cholesterol level in our study was 336.67 mg/dl (control group). In this phase, cholesterol level of the sea bream, fed with ‰2 MOSaddition was significantly lo wer (P˃0.05) than the normal range.…”

“…ALP ranged fro m 250-334 UI/l in the present trial was higher than the reported value (58-125 UI/l) by Peres et al (2013). After 24 hours of food deprivation in the current trial, glucose levels in test fish were similar to levels reported in prev ious studies (Peres et al, 1999, Sitja-Bobadilla, et al, 2005Peres et al, 2013).…”

“…Plas ma biochemistry parameters play an important role in provid ing an insight into the nutritional, physiological and health status of the animals and may help in determining the suitability of feeding practices, husbandry conditions, presence of acute or chronic stressors and pathogenic man ifestations (Peres, Santos, & Oliva-Teles, 2013). No difference (P˃0.05) in blood parameters was observed among trial groups.…”

“…In our study, the urine values were higher than reported values for Mugil cephalus (Francesco, Satheeshkumar, Senthil, Caterina, & Giuseppe, 2012), but they showed similarity with the results for Synodontis membranacea (Owolab i, 2011). Peres et al (2013) reported that AST (U L -1 ) level as 64.0 (28-134), U L -1 for juvenile sea bream which were not fed for 24 hours. AST values in our study were comparable with Peres et al (2013).…”

“…Peres et al (2013) reported that AST (U L -1 ) level as 64.0 (28-134), U L -1 for juvenile sea bream which were not fed for 24 hours. AST values in our study were comparable with Peres et al (2013). Although Peres et al (2013) reported cholesterol level in juvenile sea bream (left hungry for 24 hours) as 361.6 mg/dl (341-407), the highest cholesterol level in our study was 336.67 mg/dl (control group).…”