Selected serum oxidative stress biomarkers in dogs with non‐food‐induced and food‐induced atopic dermatitis

Almela, Ramón M.; Rubio, Camila Peres; Cerón, José J.; Ansón, Agustina; Tichy, Alexander; Mayer, Ursula

doi:10.1111/vde.12525

Cited by 10 publications

(14 citation statements)

References 47 publications

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“…As precision medicine develops, other promising diagnostic approaches for identifying AD include the use of molecular techniques on serum and blood samples, evaluating selected biomarkers or establishing a molecular profile or a metabolic fingerprint of atopic dogs versus healthy subjects . Whether those biomarkers are truly specific and would be reliable as a diagnostic tool is still to be determined, as well as their possible use as therapeutic targets for cAD.…”

Section: Discussionmentioning

confidence: 99%

Reliability of different sets of criteria in diagnosing canine atopic dermatitis applied to a population of 250 dogs seen in a veterinary teaching hospital

Brément

Laly

Combarros

et al. 2019

Veterinary Dermatology

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Background -Different sets of criteria are available in veterinary dermatology for the diagnosis of canine atopic dermatitis (cAD); there are limited data to assess their reliability.Hypothesis/Objectives -The aim of this study was to assess the discriminative and predictive ability of four sets in diagnosing cAD in a population of dogs with different skin diseases.Animals -Two hundred and fifty dogs examined in the dermatology unit of a veterinary teaching hospital.Methods and materials -Dogs were diagnosed with cAD, flea infestation (FI), flea bite hypersensitivity (FBH), sarcoptic mange (SM) and other skin diseases (OD). FI, FBH and SM were pooled in one group of dermatoses mimicking cAD (MD). All nonatopic dogs were grouped as a "global population" (GP). Four criteria sets were applied to all dogs. For each set of criteria, sensitivity, specificity, positive and negative predictive values (PPV, NPV), likelihood ratios (LR) and diagnostic odds ratio (DOR) were calculated.Results -When applied to the GP, sensitivities ranged from 54 to 83%, specificities from 68 to 75% and PPVs from 35 to 43%. NPVs ranged from 87 to 94%. LRs and DORs were poor. When applied to MD, sensitivities remained unchanged, specificities and PPV were mildly higher, whereas NPVs were mildly lower and LRs and DORs were comparable.Conclusions and clinical importance -This study showed that each set of criteria had a low diagnostic reliability when used alone. The use of more discriminant criteria integrated into a thorough clinical approach excluding MDs should be considered.

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Section: Discussionmentioning

confidence: 99%

Reliability of different sets of criteria in diagnosing canine atopic dermatitis applied to a population of 250 dogs seen in a veterinary teaching hospital

Brément

Laly

Combarros

et al. 2019

Veterinary Dermatology

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“…To evaluate lipid peroxidation, MDA analysis was performed, where 250 μL of serum was added to 400 μL of perchloric acid 1 (35%) and taken to water bath (37°C, 01h). The mixture was centrifuged (1400 g; 10 min) and 600 μL of the supernatant was added to 200 μL of 1.2% thiobarbituric acid 2 . This mixture was taken to water bath (95°C, 30 min).…”

Section: Blood Sampling and Hemato-biochemical Analysismentioning

confidence: 99%

“…The activation of inflammatory cells induces the production of free radicals, which may oxidate cellular membrane and potentially contributes to immunopathogenesis of CAD [2,5,15]. To control the oxidative skin damage, enzymatic and nonenzymatic antioxidant molecules are activated in order to improve the defense systems and neutralize free radicals [21].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Systemic and Cutaneous Inflammatory Immune Response in Canine Atopic Dermatitis

Ferreira

Lima-Verde

Júnior

et al. 2021

Acta Scientiae. Vet.

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Background: Canine atopic dermatitis (CAD) is a chronic and inflammatory disease present in veterinary dermatological practice. The inflammation in CAD is triggered by environmental allergens and skin microorganisms, which are responsible for the worsening of cutaneous lesions. This continuous activation of skin inflammatory process can induce the production of free radicals that also contribute to cellular damage and ultimately leads to changes in blood parameters in dogs with CAD. Although there are reports of inflammatory parameters in CAD, there are a lack of studies correlating skin lesions, blood leukocytes and oxidative stress. Based on that,this study aimed to evaluate the integumentary and systemic inflammatory response in dogs with atopic dermatitis. Materials, Methods & Results: Dogs with confirmed diagnosis of canine atopic dermatitis (n = 10) were divided in two groups according to CADESI-IV: AI, with CADESI between 0-10, AII, with CADESI between 10-34, and control group (n = 5). Blood-biochemical and histological analysis were performed to access systemic and cutaneous inflammatory response. AII group tended to higher neutrophil and eosinophil counts, as well as neutrophil/lymphocyte ratio (NLR) when compared to AI. The albumin was lower in AII compared to AI and control (P < 0.05), while total bilirubin and malondialdehyde (MDA) did not differ between groups. NLR (r = 0.64 and P = 0.04) and MDA (r = 0.54 and P = 0.1) were positively correlated with CADESI, while albumin was negatively correlated with CADESI (r = -0.79 and P = 0.005). Histopathological analysis revealed a larger number of neutrophils, macrophages and mast cells in AI and AII than in control group (P < 0.05). Discussion: In this study it was possible to evaluate the systemic and cutaneous leukocyte dynamics in CAD. Skin inflammation induces the production of chemotactic molecules contribute to neutrophil outflow from blood vessel toward the affected tissue, which can be visualized as perivascular inflammatory infiltrate and exocytosis. At systemic level, there was a tendency to increase in total leukocytes and circulating neutrophil count in group AII, as well as in neutrophil/lymphocyte ratio (NLR), when compared to AI. The NLR is a widely available and inexpensive laboratory biomarker that quantifies systemic inflammation, being used in human medicine to evaluate prognosis in different types of cancer. In our study, dogs in AII group showed an increased NLR compared to AI and control, which demonstrates the influence of skin injury in systemic parameters. Furthermore, AII group is composed of dogs with greater lesion state, which reflects in higher NLR values. Since this disease is known by its chronicity and may remain stable for years, NLR may be a novel biomarker to evaluate acute exacerbation in CAD and could potentially explain why some patients have longer crisis duration and frequent flares. The maintenance of the inflammatory state also induces the production of oxidizing substances, which possibly exceed the total antioxidant capacity, generating a situation of oxidative stress, which can result in damage to membrane lipids and release of their products. MDA is reliable and is the most commonly used marker of the overall lipid peroxidation level and the presence of oxidative stress. This result may be related with the antioxidant system, such as albumin and bilirubin, which was able to promote an efficient control of oxidant substances. In conclusion, the presented data demonstrated an inflammatory process progression as well as introduced NLR as a potential marker of disease exacerbation in CAD.

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“…The development of CAD has been suggested to be genetically predisposed in canines, as well as further modulated by epigenetic factors (18). Phenotypically, the disease manifests itself differently in each individual (21), although there is a relatively consistent trait of elevated concentrations of the antibody immunoglobulin E across both atopy types and species (22,23). Atopic dermatitis (AD) has been associated with several of the classic markers of metabolic syndrome (MetS) found both in humans (24) and in canines (25).…”

Section: Introductionmentioning

confidence: 99%

“…Nutrition has been shown to have a vital role in determining the development of MetS through modulating metabolic pathways that have been attributed to the development of AD (22). CAD typically comprises both food-induced AD and non-food-induced AD (23). Although physiologically indistinguishable (19), they can be differentiated with the dietrestriction provocation trial (20).…”

Section: Introductionmentioning

confidence: 99%

Targeted Metabolomics With Ultraperformance Liquid Chromatography–Mass Spectrometry (UPLC-MS) Highlights Metabolic Differences in Healthy and Atopic Staffordshire Bull Terriers Fed Two Different Diets, A Pilot Study

et al. 2020

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Background: While anecdotal evidence has long claimed that a raw meat-based diet (RMBD) improves the metabolic health of canines, no rigorous scientific study has clarified this issue. Canine atopic dermatitis (CAD) has also been linked to metabolic health, but its relation to diet remains poorly understood. This study investigates whether dietary choice is linked to metabolic health in healthy and CAD-diagnosed canines via targeted serum and urine metabolomic analysis of polar, non-ionic metabolites, as well as whether the underlying CAD condition modulates the response to nutritional intake. Materials and Methods: Serum metabolites of client-owned Staffordshire bull terriers, divided into CAD-diagnosed (n = 14) and healthy (n = 6) cohorts, were studied. Urine metabolites of a subset of the CAD-diagnosed canines (n = 8) were also studied. The canines were split into two cohorts based on diet. The first cohort were fed a commercially available high-fat, moderate-protein, low-carbohydrate RMBD (n = 11, CAD diagnosed n = 8, healthy n = 3). Those in the second cohort were fed a commercially available moderate-fat, moderate-protein, high-carbohydrate kibble diet (KD) (n = 9: CAD diagnosed n = 6, healthy n = 3). The diet intervention period lasted approximately 4.5 months (median 135 days). Statistical analyses of the serum profiles across all dogs (n = 20) and the urine profiles of the CAD-diagnosed subset (n = 8) were performed. Results and Discussion: The KD cohort was found to have higher concentrations of methionine than the RMBD cohort, both in serum (all dogs, p < 0.0001) and in urine (CAD-only cohort, p < 0.0002), as well as cystathionine and 4-pyridoxic acid. Methionine plays important roles in homocysteine metabolism, and elevated levels have been implicated in various pathologies. The CAD (n = 14) cohort dogs showed starker Moore et al. Canine Targeted Metabolomics Diet Intervention metabolic changes in response to diet regarding these pathways compared to the healthy (n = 6) cohort. However, there was no significant change in CAD severity as a result of either diet. Likely due to the higher meat content of the RMBD, higher concentrations of several carnitines and creatine were found in the RMBD cohort. Citrulline was found in higher concentrations in the KD cohort. Our findings provide insight into the relationship between diet and the serum and urine metabolite profiles of canines. They also suggest that neither diet significantly affected CAD severity.

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Selected serum oxidative stress biomarkers in dogs with non‐food‐induced and food‐induced atopic dermatitis

Abstract: These findings suggest that OS could play a role in the pathogenesis of canine NFIAD and FIAD. In addition, the evaluation of sbOS could be useful for precision medicine to help to detect atopic dogs that might benefit from antioxidant-targeted therapies.

Cited by 10 publications

References 47 publications

Reliability of different sets of criteria in diagnosing canine atopic dermatitis applied to a population of 250 dogs seen in a veterinary teaching hospital

Reliability of different sets of criteria in diagnosing canine atopic dermatitis applied to a population of 250 dogs seen in a veterinary teaching hospital

Analysis of Systemic and Cutaneous Inflammatory Immune Response in Canine Atopic Dermatitis

Targeted Metabolomics With Ultraperformance Liquid Chromatography–Mass Spectrometry (UPLC-MS) Highlights Metabolic Differences in Healthy and Atopic Staffordshire Bull Terriers Fed Two Different Diets, A Pilot Study

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