Selected sperm traits are simultaneously altered after scrotal heat stress and play specific roles in in vitro fertilization and embryonic development

Lucio, A. C.; Alves, Bênner Geraldo; Alves, Kele Amaral; Martins, Muller Carrara; Braga, Lucas S.; Miglio, Luisa; Silva, Thiago Henrique da; Jacomini, José Octávio; Beletti, Marcelo Emı́lio

doi:10.1016/j.theriogenology.2016.03.015

Cited by 32 publications

(20 citation statements)

References 64 publications

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“…In rams, simulated circadian thermal stress conditions (from 38°C to 44 °C after a daily schedule for 8 weeks) in climatic chambers altered the percentage of rapidly motile sperm as well as the linearity and the average path velocity of spermatozoa (De et al, ). In bulls, after a 72‐hr scrotal insulation period (i.e., the scrotum was wrapped with cotton layers and covered with a plastic bag) or a high temperature exposure (35.5°C and 50% RH for 7 weeks), there were simultaneous alterations in either spermatogenesis and semen characteristics or several sperm parameters affecting IVF and in vitro embryo development (Lucio et al, ; Rhynes & Ewing, ). In response to high temperatures, animals tend to maintain homeothermy through physiological adjustments.…”

Section: Male Reproductive Apparatusmentioning

confidence: 99%

Heat stress, a serious threat to reproductive function in animals and humans

Boni

2019

Molecular Reproduction Devel

106

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Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female components, fertilization mechanisms as well as the early and late stages of embryofetal development. This occurrence causes great economic damage in livestock, and, in wild animals creates selective pressure towards the advantages of better-adapted genotypes to the detriment of others. Humans undergo several types of stress, including heat, and these represent putative causes of ongoing progressive decay in procreation; an increasing number of remedies in the form of antioxidant preparations are now being proposed to counteract the effects of stress. This review aims to describe the results of the most recent studies that aimed to highlight these effects and to draw information on the mechanisms acting as the basis of this problem from a comparative analysis. K E Y W O R D S follicle, global warming, heat stress, mitochondria, oocyte, reproductive activity, spermatozoa Mol Reprod Dev. 2019;86:1307-1323.wileyonlinelibrary.com/journal/mrd

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Section: Male Reproductive Apparatusmentioning

confidence: 99%

Heat stress, a serious threat to reproductive function in animals and humans

Boni

2019

Molecular Reproduction Devel

106

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“…Intensive genetic selection for high milk production significantly shortened the thermoregulatory competence in dairy cows (De Rensis & Scaramuzzi, 2003), which is often detected as negative effects in reproductive parameters both in vivo and in vitro in cows and embryos under heat stress (HS) conditions (Chebel et al, 2004;De Rensis, Garcia-Ispierto, & Lopez-Gatius, 2015;Nabenishi et al, 2011;Rivera & Hansen, 2001). High temperature alters follicular development (Roth, 2015), steroidogenesis (Bridges, Brusie, & Fortune, 2005), and decreases the quality of females (Al-Katanani, Paula-Lopes, & Hansen, 2002) and male (Lucio et al, 2016) gametes. In vitro-produced bovine embryos demonstrated developmental inhibition when exposed to temperatures considered as heat-stressed cows in vivo, reducing cleavage and blastocyst rates (Rivera & Hansen, 2001).…”

mentioning

confidence: 99%

Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro

Amaral

Koch

Júnior

et al. 2020

Molecular Reproduction Devel

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Interferon tau (IFNT) is the cytokine responsible for the maternal recognition of pregnancy in ruminants and plays a role modulating embryo–maternal communication in the oviduct inducing a local response from immune cells. We aimed to investigate IFNT production, reactive oxygen species, and oxidative stress under the influence of heat stress (HS) during different stages of bovine in vitro embryo production. HS was established when the temperature was gradually raised from 38.5°C to 40.5°C in laboratory incubator, sustained for 6 hr, and decreased back to 38.5°C. To address the HS effects on IFNT production, reactive oxygen species, and oxidative stress, ovaries from a slaughterhouse were used according to treatments: control group (38.5°C); oocytes matured under HS; oocytes fertilized under HS; zygotes cultured in the first day under HS; and cells submitted to HS at oocyte maturation, fertilization, and the first day of zygote culture. The HS negatively affected cleavage and blastocyst rates, in all HS groups. On Day 7, all HS‐treated embryos showed decrease IFNT gene and protein expressions, whereas reactive oxygen species were increased in comparison to the control. In conclusion, the compromised early embryo development due to higher temperatures during in vitro oocyte maturation, fertilization, and/or zygote stage have diminished IFNT expression and increased reactive oxygen species in bovine.

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“…To evaluate the efficiency of the samples, 50 PIVEs were performed using approximately 3,000 viable oocytes (on average 20 oocytes per bull per IVEP). Oocytes collected from slaughterhouse ovaries by follicular aspiration were subjected to maturation (IVM), fertilization (IVF), and in vitro culture (IVC) according Lucio et al (2016). The cumulus oocytes complexes (COCs) were aspirated from follicles between 3 and 8 mm in diameter.…”

Section: Methodsmentioning

confidence: 99%

Micro-RNAs differentially expressed in comparative analysis of sperm samples with high and low efficiency in the in vitro production of bovine (Bos Taurus) embryos

Silva¹,

Gomes²,

Fujimura³

et al. 2019

Biosci. J.

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Infertility or subfertility in bovine males may be related to spermatic microRNAs (miRNAs), whose function seems to be associated with the regulation of gene expression, degradation or storage of messenger RNAs (mRNAs) for later translation into early embryonic development. Thus, the purpose of this study was to identify differentially expressed miRNAs in semen samples from bulls (Bos taurus) with low and high efficiency in the in vitro embryo production (IVEP) and to evaluate if they can be used as markers of semen efficiency for IVEPs. In order to identify miRNA markers of semen efficiency in the in vitro embryo production, eight semen samples from each animal, one bull with high and two bulls with low efficiency in IVEPs were used to perform the RNAseq technique for miRNAs. Initially the samples were washed with PBS to remove the extender semen and subsequently were submitted to RNA extraction protocols performed according to procedures described by mirVana™ miRNA Isolation Kit. Then, the amplification of the miRNAs was carried out, not to mention the preparation of the library (Ion Total RNA-Seq Kit v2), the PCR emulsion reaction, enrichment, as well as the injection of the sample on the chip by the Ion Chef equipment. The sequencing was done on Ion Proton equipment. The comparison between the samples was established using two methodologies for searching for targets to increase the robustness of the analytical procedure: the miRanda program using as cutoff minimum free energy of the hybridization-20 kcal/Mol, 100% of identity between nucleotides 2 and 8 of the miRNA, and the RNAhybrid program, using as cutoff minimum free energy of hybridization-20 kcal/mol. In sum, 1306 miRNAs were identified in the samples. The bta-miR-380-5p, bta-miR-155, bta-miR-30c and bta-miR-34a genes were identified by the Bioinformatics as being strongly differentially expressed between the groups, indicating that these genes may present themselves as possible efficiency markers. However, it has become clear that there is no single miRNA that marks different types and causes of fertility problems.

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Selected sperm traits are simultaneously altered after scrotal heat stress and play specific roles in in vitro fertilization and embryonic development

Cited by 32 publications

References 64 publications

Heat stress, a serious threat to reproductive function in animals and humans

Heat stress, a serious threat to reproductive function in animals and humans

Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro

Micro-RNAs differentially expressed in comparative analysis of sperm samples with high and low efficiency in the in vitro production of bovine (Bos Taurus) embryos

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