Selecting better diagnostic kits for diagnosis of malarial parasites at point of care

Mallepaddi, Prudhvi Chand; Maity, Soumendra Nath; Poonati, Revathi; Pyadala, Nagababu; Polavarapu, Rathnagiri; Mangamuri, Usha Kiranmayi; Poda, Sudhakar

doi:10.1007/s13205-018-1566-7

Cited by 4 publications

(1 citation statement)

References 38 publications

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“…Overall the microscopy and malaria antigen-based lateral flow assay exhibited similar sensitivity, specificity, PPV, NPV and efficiency; their results are comparable with our study. 13 A study in Kenya conducted in 2016 showed that in comparison to microscopy, the sensitivity of eight RDTs to detect malaria parasites was 90.3-94.8 %, and the specificity was 73.3-79.3 %. 14 Ojurongbe et al, compared PCR, thick film microscopy and RDTs to detect Malarial parasites.…”

Section: Discussionmentioning

confidence: 99%

Accuracy of Rapid Diagnostic Test for Malaria Compared to Microscopy

Shah

Waris

Mushtaq³

et al. 2022

PAFMJ

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Objective: To determine the diagnostic accuracy of the Rapid Diagnostic Test in the detection of malaria by taking routine microscopy as a gold standard. Study design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Pakistan Naval Ship Rahat Hospital, Karachi Pakistan, from Aug 2018 to Jan 2019. Methodology: Patients diagnosed as a case with malaria were included. Malaria was confirmed by using a Rapid detection test and microscopy. Mean and standard deviations were calculated for the quantitative variables like age. Frequencies and percentages were calculated for the qualitative variables like gender. Sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy were calculated. Results: Out of 175 patients in the study, the mean age was 39.30 ± 11.29 years. 85 (48.6%) were male, and 90 (51.4%) were female. Out of 175 patients, sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy of the rapid diagnostic test for malaria, keeping microscopy as the gold standard was found to be 87.2%, 74.15%, 67.34%, 85.71% and 80.57% respectively. Conclusion: Rapid immune-capture tests can have excellent applicability in malaria diagnosis. Field workers can reliably read such tests without any supervision. It appears to satisfy the desired criteria of being rapid, easy to perform, interpret and reasonably accurate.

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Section: Discussionmentioning

confidence: 99%

Accuracy of Rapid Diagnostic Test for Malaria Compared to Microscopy

Shah

Waris

Mushtaq³

et al. 2022

PAFMJ

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Rapid diagnostic tests forPlasmodium vivaxmalaria in endemic countries

Agarwal

Choi

Johnson

et al. 2020

Cochrane Database of Systematic Reviews

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Background Plasmodium vivax ( P vivax ) is a focus of malaria elimination. It is important because P vivax and Plasmodium falciparum infection are co‐endemic in some areas. There are asymptomatic carriers of P vivax, and the treatment for P vivax and Plasmodium ovale malaria differs from that used in other types of malaria. Rapid diagnostic tests (RDTs) will help distinguish P vivax from other malaria species to help treatment and elimination. There are RDTs available that detect P vivax parasitaemia through the detection of P vivax‐ specific lactate dehydrogenase (LDH) antigens. Objectives To assess the diagnostic accuracy of RDTs for detecting P vivax malaria infection in people living in malaria‐endemic areas who present to ambulatory healthcare facilities with symptoms suggestive of malaria; and to identify which types and brands of commercial tests best detect P vivax malaria. Search methods We undertook a comprehensive search of the following databases up to 30 July 2019: Cochrane Infectious Diseases Group Specialized Register; Central Register of Controlled Trials (CENTRAL), published in the Cochrane Library; MEDLINE (PubMed); Embase (OVID); Science Citation Index Expanded (SCI‐EXPANDED) and Conference Proceedings Citation Index‐Science (CPCI‐S), both in the Web of Science. Selection criteria Studies comparing RDTs with a reference standard (microscopy or polymerase chain reaction (PCR)) in blood samples from patients attending ambulatory health facilities with symptoms suggestive of malaria in P vivax ‐endemic areas. Data collection and analysis For each included study, two review authors independently extracted data using a pre‐piloted data extraction form. The methodological quality of the studies were assessed using a tailored Quality Assessment of Diagnostic Accuracy Studies‐2 (QUADAS‐2) tool. We grouped studies according to commercial brand of the RDT and performed meta‐analysis when appropriate. The results given by the index tests were based on the antibody affinity (referred to as the strength of the bond between an antibody and an antigen) and avidity (referred to as the strength of the overall bond between a multivalent antibody and multiple antigens). All analyses were stratified by the type of reference standard. The bivariate model was used to estimate the pooled sensitivity and specificity with 95% confidence intervals (CIs), this model was simplified when studies were few. We assessed the certainty of the evidence using the GRADE approach. Main results We included 10 studies that ...

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Metallic nanoparticles in malaria treatment: advances in therapeutics, diagnostics, and future prospects

Sinha,

Sehgal,

Medhi

2024

AAPS Open

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Malaria remains one of the causes of extreme mortality in southern Africa and Southeast Asia. Despite extreme efforts to control and eliminate malaria, the appearance of drug-resistant parasites and their spread is a major concern. In this context various therapeutic, diagnostics, and technological advancements need to be studied and implemented in the near future. Nanoparticles have a crucial role in enhancing intracellular retention, subcellular target specificity, and even drug escape from intracellular enzymatic inactivation. Additionally, the selectivity and specificity of anti-malarial against the target were increased by the functionalization of nanoparticles, which led to improved safety and efficacy. Recent studies show the efficacy of metallic nanoparticles against the vector Anopheles mosquito and the malaria parasite Plasmodium species. So, here in the present review, we will discuss the various metal-based nanoparticles that have been studied for malaria treatment or used for the development of detection and diagnosis between the year 2013 and till date. This review also covers the future prospects of these nanoparticles in this field.

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Selecting better diagnostic kits for diagnosis of malarial parasites at point of care

Cited by 4 publications

References 38 publications

Accuracy of Rapid Diagnostic Test for Malaria Compared to Microscopy

Accuracy of Rapid Diagnostic Test for Malaria Compared to Microscopy

Rapid diagnostic tests forPlasmodium vivaxmalaria in endemic countries

Metallic nanoparticles in malaria treatment: advances in therapeutics, diagnostics, and future prospects

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