Selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast Rhodosporidium toruloides NCYC 921 using flow cytometry

Freitas, Cláudia; Parreira, Teresa Margarida; Roseiro, J. Carlos; Reis, Alberto; Silva, Teresa Lopes da

doi:10.1016/j.biortech.2014.02.071

Cited by 64 publications

(43 citation statements)

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“…The closest species genetically to those studied in this work are Rhodotorula and Rhodosporidium (97%), both characterized as lipid and carotenoid producers (Saenge et al 2011, Freitas et al 2014. A more distant similarity (81%) was found with species of the Thraustochytriaceae family, characterized by high levels of DHA production (Chi et al 2007).…”

Section: Discussionmentioning

confidence: 56%

“…Rhodosporidium toruloides and Rhodotorula glutinis produce around 70 and 33.7% of total lipids in dry weight, respectively. However, the fatty acids produced by these species include myristic, palmitic, palmitoleic, steric, oleic, linoleic and -linoleic acids (Li et al 2006, Freitas et al 2014, in contrast to the strains studied here that produce high concentrations of DHA and EPA, neither of which have been described in Rhodotorula and Rhodosporidium (Li et al 2007, Hu et al 2009). …”

Section: Discussionmentioning

confidence: 80%

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Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Pino¹,

Socias²,

González³

2015

Rev. biol. mar. oceanogr.

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Resumen.-La creciente demanda de ácidos grasos poliinsaturados (AGPI) provocada por la escasez de pesca extractiva, ha impulsado la obtención de fuentes alternativas sustentables para abastecer su mercado. Los Thraus tochytridos y levaduras marinas (fungoides marinos), son potenciales productores de lípidos y carotenoides de importancia comercial. Desde una colección de 41 cepas de fungoides marinos aisladas en el Sistema de Corriente de Humboldt, se determinó la capacidad de producir DHA (ácido docosahexanoico), EPA (ácido eicosapentanoico) y CT (carotenoides totales) en cultivos líquidos comercial (MS) y alternativos (PDB, GAM, BAM y MCM). Los resultados indican que el medio MS y PDB, presentaron las mayores velocidades de crecimiento (0,02 h -1 ). No obstante, el medio MS presenta estos parámetros cinéticos a 16ºC y el PDB a 37°C. Trece de las cepas analizadas presentaron una alta capacidad para producir DHA (hasta 23% peso seco) y CT (hasta 18% peso seco), comparables a los niveles observados en Schizochytrium sp. KH105 y Rhodosporidium toruloides. Adicionalmente, todas las cepas ensayadas también produjeron pequeñas cantidades de EPA (hasta 0,3% peso seco). La microscopia electrónica revela que la cepa C36 es morfológicamente consistente con levaduras y su secuencia parcial del gen ribosomal 18s presenta un 97% de similitud con el género Rhodotorula, el que hasta ahora no ha sido reportado como productor de DHA y EPA. Finalmente, las cepas C36, C22 y C4 son interesantes objetos de estudio para escalar su producción y proyectar su uso comercial, como por ejemplo para suplementar en omega-3 y carotenoides alimentos para consumo humano, animales de corral o para estadios larvales de peces cultivados y pigmentar la carne del salmón, entre otros.Palabras clave: Fungoides marinos, DHA, EPA, carotenoides totales, Rhodotorula, parámetros cinéticos Abstract.-Declining fishing yields have pushed the search for sustainable alternative sources for polyunsatura ted fatty acids (PUFAs). Thraustochytrids and marine yeasts (marine fungoid protists) are potential commercial sources of lipids and carotenoids. It was determined the capacity of a collection of 41 strains of marine fungoid isol ated in the Humboldt Current System, to produce DHA (docosahexanoic acid), EPA (eicosapentanoic acid) and CT (total carotenoids) in commercial growing media (MS) and alternative growing mediums (PDB, GAM, BAM and MCM). The media MS and PDB exhibited the highest growth rate (0.02 h -1 ), at 16 and 37°C, respectively. Thirteen of the studied strains showed high capacity to produce DHA (up to 23% dry weight) and CT (up to 18% dry weight), comparable to levels observed in Schizochytrium sp. KH105 and Rhodosporidium toruloides. Additionally, all studied strains produce small amounts of EPA (up to 0.3% of dry weight). Scanning electron microscopy reveals that strain C36 is morphologically consistent with yeasts, while partial sequencing of the 18s ribosomal gene shows 97% similarity to the genus Rhodotorula, which has not been reported until n...

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 80%

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Pino¹,

Socias²,

González³

2015

Rev. biol. mar. oceanogr.

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“…They showed that the overall biodiesel production cost was economically competitive (US$ 0.76/l) to that of vegetable biodiesel (US$ 0.81/l) and the yield of biodiesel is 6.3-fold higher (4172 l/ha of cultivated sugarcane) than that obtained from yield of soybean biodiesel (661 l/ha of cultivated soybean) [65]. Besides this various low-cost raw materials such as sugarcane molasses (SCM) are extensively used as raw materials for oleaginous yeast due to its availability and high sugars contents [66]. It has been earlier reported that Rhodosporidium toruloides could produce 63.2% and 56.5% lipid content (w/w) when hydrolysates of non-edible crops Cassava and Jerusalem artichoke respectively were provided [67].…”

Section: Low-cost Substrates Utilized By Oleaginous Yeasts To Producementioning

confidence: 99%

Assessment of fuel properties on the basis of fatty acid profiles of oleaginous yeast for potential biodiesel production

Patel

Arora

Mehtani

et al. 2017

Renewable and Sustainable Energy Reviews

196

100

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A B S T R A C TOver the last decade, there has been a huge upsurge of interest in sustainable production of biomass-based biofuels to fulfill the existing energy demand and simultaneously reducing the environmental deterioration. Earlier, vegetable oils and animal fats were utilized for biodiesel production, but due to food crisis and environmental sustainability, renewable sources such as neutral lipid derived from microbes are gaining much attention for budding biodiesel industries. Among various types of microorganisms, oleaginous yeasts are more promising feedstock to accomplish the current demand of biodiesel production and utilize a large number of cost-effective renewable substrates for their growth and lipid accumulation. However, biodiesel obtained from oleaginous yeasts have certain restrictions regarding their commercial utilization due to their unstable fuel properties such as oxidative stability, cetane number, viscosity and low-temperature performance etc. Numerous articles have been published in the public domain describing the fatty acid profiles of oleaginous yeast as feedstock for biodiesel production. However, the evaluation of quality parameters of biodiesel obtained from oleaginous yeasts is still in infancy. Although there is a huge disparity in a number of papers published for biodiesel production yet the reporting performance on diesel engines need to be verified in details. In this review article, attempt has been made to assess the important biofuel properties on the basis of the fatty acid profile of oleaginous yeast. Thus this evaluation would provide a guideline to the biodiesel producer to improve the production plans related to feedstocks for oleaginous yeast, culture conditions and biodiesel blending.

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“…In particular, red yeasts of the genera Phaffia, Rhodotorula and Sporidibolus exhibit production of considerable amounts of carotenoids (Davoli et al, 2004;Dias et al, 2016), rendering these yeast strains potential sources for industrial pigment production (Freitas et al, 2014;Yen and Chang, 2015).…”

Section: Introductionmentioning

confidence: 99%

A single desaturase gene from red yeast Sporidiobolus pararoseus is responsible for both four- and five-step dehydrogenation of phytoene

Zhang

Song

et al. 2016

Gene

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Selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast Rhodosporidium toruloides NCYC 921 using flow cytometry

Cited by 64 publications

References 13 publications

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Assessment of fuel properties on the basis of fatty acid profiles of oleaginous yeast for potential biodiesel production

A single desaturase gene from red yeast Sporidiobolus pararoseus is responsible for both four- and five-step dehydrogenation of phytoene

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