Selecting the optimal time to perform biopsy for preimplantation genetic testing

Scott, Katherine; Hong, K.H.; Scott, Richard T.

doi:10.1016/j.fertnstert.2013.07.004

Cited by 129 publications

(112 citation statements)

References 32 publications

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“…This embryo was transferred and the patient delivered a healthy baby carrying a chromosomal balanced translocation (tested by amniocentesis). There may be two reasons of this embryo, one is mosaicism [6][7][8][9][10], the other is self-correction during embryo development. Several reasons have been proposed for aneuploidy selfcorrection during later developmental stages, including primary misdiagnosis, aneuploidy allocation in the trophectoderm, cell growth advantage of diploid cells in mosaic embryos, lagging of aneuploid cell division, extrusion or duplication of an aneuploid chromosome, and abundance of DNA repair gene products [16].…”

Section: Discussionmentioning

confidence: 99%

“…At present, blastocyst stage is the optimal time to perform biopsies for preimplantation genetic testing [10]. Trophectoderm biopsies do less harm to development of the embryo [20], while blastocyst biopsy can obtain multiple trophectoderm cells, resulting in an increased number of DNA copies in the biopsy specimen, and therefore more accurate results compared with a single blastomere cell [21].…”

Section: Discussionmentioning

confidence: 99%

“…Day 4 embryos have also been studied, with extensive abnormalities observed [9]. When embryos develop to the blastocyst stage they still show chromosomal abnormalities and mosaicism, although the proportion decreases [10].…”

Section: Introductionmentioning

confidence: 99%

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Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Huang

Zhao

Wang

et al. 2015

J Assist Reprod Genet

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Purpose To investigate chromosomal characteristics in the same embryos at cleavage and blastocyst stage. Methods Six PGD/PGS cycles were retrospective studied, including five chromosomal translocation PGD cycles and one recurrent abortion PGS cycle. The cleavage embryos were biopsied one blastomere at day 3, followed by blastocyst culture. Trophectoderm cell biopsy was performed when embryos developed into the blastocyst stage. Whole genome amplification and 24-chromosomal analysis by CGH/SNP microarray was performed on each blastomere and trophectoderm cells. Results After PGD/PGS, only one couple had no euploid embryos to transfer. Five couples delivered a healthy, balancedkaryotype baby. A total of 18 embryos had both blastomere and trophectoderm cell reliable results available. Of the 18 embryos, eleven embryos contained identical chromosomes from cleavage stage to blastocyst stage and six embryos contained almost identical chromosomes . Only one embryo presented opposite chromosomal results for the cleavage and blastocyst stage, with abnormal chromosomes in the blastomere but normal chromosomes in trophectoderm cells. Conclusions Most embryos maintain chromosomal stability during embryonic development. Compared to cleavage stage, blastocyst stage may provide more reliable aneuploidy results.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Huang

Zhao

Wang

et al. 2015

J Assist Reprod Genet

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“…Both PGD and PGS require access to the DNA of the preimplanted embryos, which is provided by either polar body biopsy, blastomere biopsy of cleavagestage embryos, or trophectoderm biopsy of blastocysts. PGS is mainly performed on trophectodermal cells biopsied from blastocysts to allow a better representation of the embryo ploidity [3][4][5][6][7]. In contrast, PGD for severe monogenic disorders is usually performed on blastomeres biopsied from day 3 embryos.…”

Section: Introductionmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

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“…The blastocyst stage is the optimal stage for performing biopsies for preimplantation genetic screening (PGS) [1]. The high efficiency of PGS at the blastocyst stage has being reported as a key factor determining the growing clinical application of this strategy worldwide [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

The optimal timing of blastocyst vitrification after trophectoderm biopsy of preimplantation genetic screening

Lee

2016

Fertility and Sterility

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Selecting the optimal time to perform biopsy for preimplantation genetic testing

Cited by 129 publications

References 32 publications

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Chromosomal characteristics at cleavage and blastocyst stages from the same embryos

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

The optimal timing of blastocyst vitrification after trophectoderm biopsy of preimplantation genetic screening

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