Selection and analytical applications of aptamers

Hamula, Camille; Guthrie, Jeffrey; Zhang, Hongquan; Li, Xing‐Fang; Le, X. Chris

doi:10.1016/j.trac.2006.05.007

Cited by 268 publications

(152 citation statements)

References 48 publications

Supporting

Mentioning

147

Contrasting

Unclassified

Order By: Relevance

“…These help to remove undesirable adsorbed oligonucleotides from the poolby matrixes that used for immobilizing the targets and distinction of similar structures [43]. In addition, in order to enhance targets applicability, other variants SELEX used are: Whole bacteria-SELEX [43], blended SELEX [44], TECS-SELEX [45], complex target SELEX [46], toggle SELEX targetswitching [47], expressions cassette also known as SELEX-SAGE [48], the mirror-image SELEX or Spiegelmer technology [49], whole cell-SELEX [50]. Efficacy is the prerequisite for any aptamer-based design; several SELEX strategies such as non-SELEX or NECEEM-SELEX, CE-SELEX [51], microfluidic SELEX [52], HTS-SELEX or automated SELEX [53], FluMag-SELEX [54], in silico SELEX [55] are applicable.…”

Section: Selection Of Aptamer Probesmentioning

confidence: 99%

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Ndikubwimana¹

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

Section: Selection Of Aptamer Probesmentioning

confidence: 99%

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Ndikubwimana¹

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

“…Aptamers can bind to specific molecular targets including small molecules, proteins, nucleic acids, phospholipids and can also be targeted to complex structures such as cells, tissues, bacteria and other organisms. Because of their strong and specific binding through molecular recognition, aptamers are promising tools in molecular biology and have both therapeutic and diagnostic clinical applications [32][33][34][35]. Unfortunately, some limitations of the SELEX technique have seriously slowed down the progress of discovering aptamers [36].…”

Section: Aptamer Designmentioning

confidence: 99%

Maximum Entropy in Drug Discovery

Tseng

Tuszyński

2014

Entropy

View full text Add to dashboard Cite

Drug discovery applies multidisciplinary approaches either experimentally, computationally or both ways to identify lead compounds to treat various diseases. While conventional approaches have yielded many US Food and Drug Administration (FDA)-approved drugs, researchers continue investigating and designing better approaches to increase the success rate in the discovery process. In this article, we provide an overview of the current strategies and point out where and how the method of maximum entropy has been introduced in this area. The maximum entropy principle has its root in thermodynamics, yet since Jaynes' pioneering work in the 1950s, the maximum entropy principle has not only been used as a physics law, but also as a reasoning tool that allows us to process information in hand with the least bias. Its applicability in various disciplines has been abundantly demonstrated. We give several examples of applications of maximum entropy in different stages of drug discovery. Finally, we discuss a promising new direction in drug discovery that is likely to hinge on the ways of utilizing maximum entropy.

show abstract

“…Aptamers are often called 'synthetic antibodies' because they can mimic antibodies in a number of applications and bind their targets with affinities and specificities that can be comparable to those of antibodies: the equilibrium dissociation constants of aptamers to targets are usually in the range of picomolar (pM) to micromolar (µM), similar to those of antibodies for antigens. [209][210] However, aptamers present some advantages. In the past few years, integration of functional aptamers into nanomaterials has become a new interdisciplinary field that aims at providing new hybrid sensing systems (sensors) for specific and sensitive molecular recognition.…”

Section: Cnt-aptasensorsmentioning

confidence: 99%

Carbon Nanotubes as Platforms for Biosensors with Electrochemical and Electronic Transduction

Pujadó

2012

Springer Theses

View full text Add to dashboard Cite

CCa ar rb bo on n n na an no ot tu ub be es s a as s p pl la at tf fo or rm ms s f fo or r b bi io os se en ns so or rs s w wi it th h e el le ec ct tr ro oc ch he em mi ic ca al l a an nd d e el le ec ct tr ro on ni ic c t tr ra an ns sd du uc ct ti io on n By Mercè Pacios Pujadó Universitat Autònoma de Barcelona The present thesis entitled "CARBON NANOTUBES AS PLATFORMS FOR BIOSENSORS WITH ELECTROCHEMICAL AND ELECTRONIC TRANSDUCTION"has been performed at the laboratories of the "Sensor and Biosensor" group of Chemistry Department at "Universitat Autònoma de Barcelona" under the direction of Dra. Maria José Esplandiu Egido CSIC senior reseracher in the Institute of Nanoscience and Nanotechnology (CIN2-CSIC) and Dr. Manel del Valle Zafra, UAB Titular professor. Memòria presentada per aspirer al Grau de Doctor per Mercè Pacios PujadóDra. Maria José Esplandiu Egido Dr. Manel del Valle Zafra Bellaterra, 26th September 2011The present PhD thesis was financially supported by the Ministry of Education andScience of Spain through a FPI pre-doctoral grant (Project NAN2004-093006-C05-03and CTQ-2006-15681-C02-01). Grup de Sensors i BiosensorsUnitat de Quimica Analitica A AB BB BR RE EV VI IA AT TI IO ON NS S A AN ND D S SY YM MB BO OL LS S ABBREVIATIONS AND SYMBOLS SUMMARYThe convergence of nano and biotechnology is enabling scientific and technical knowledge for improving human well being. Within this new field of nanobiotechnology, the area of (bio)sensors is the most developed and the one which has the highest potential for short-term applications. In this context, (bio) sensors based on electrochemical principles stand out due to their marked advantages in terms of simplicity, robustness, low cost, miniaturization capability and integration to devices.These sensors are becoming very attractive for rapid and simple diagnostic, in fields such as biotechnology, clinical and environmental research.Historically, carbon has been a widely used and practical electrode material due to its desirable properties for electrochemical applications. Available in a variety of structures, carbon electrodes provide, in general, good electrical conductivity, high thermal and mechanical stability, a wide operable potential window with slow oxidation kinetics and, in many cases, electrocatalytical activity. On top of that, they are recognized as versatile and easy handling materials, and also praised by their rich surface chemistry which has been exploited to influence surface reactivity. Thus, The third and last part of the study is focused on exploiting the semiconductor character of carbon nanotubes for sensor technology by using a field effect transistor XXIX configuration (FET). The CNT-FET device has been optimized for operating in liquid environment by performing passivation protocols. This, together with the use of bifunctional pyrene linkers for the immobilization of the aptamers, has allowed sensitive electronic detection of protein/aptamer interaction. Additionally, we were also able to follow up protein adsorption and protei...

show abstract

Selection and analytical applications of aptamers

Cited by 268 publications

References 48 publications

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Maximum Entropy in Drug Discovery

Carbon Nanotubes as Platforms for Biosensors with Electrochemical and Electronic Transduction

Contact Info

Product

Resources

About