Selection and application of biological safety cabinets in diagnostic and research laboratories with special emphasis on COVID-19

Pawar, Shailesh D.; Khare, Ajay; Keng, Sachin S.; Kode, Sadhana S.; Tare, Deeksha S.; Singh, Dinesh Kumar; More, Ramesh L.; Mullick, Jayati

doi:10.1063/5.0047716

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…The specimens were used to evaluate the performance of all three SARS-CoV-2 RT-qPCR assay kits. All specimens were anonymized, and all tests were performed in Class-II Biosafety cabinets in accordance with safety regulations [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Rapid Multiplex Reverse Transcription-Quantitative Polymerase Chain Reaction Assays for SARS-CoV-2 Detection in Individual and Pooled Samples

Baek,

Park,

Lim

et al. 2023

Life

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Although coronavirus disease 2019 (COVID-19) is no longer a Public Health Emergency of International Concern (PHEIC), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has had a vast impact to date. Hence, continuous management is required, given the uncertainty caused by the potential evolution of SARS-CoV-2. Reverse transcription-quantitative PCR (RT-qPCR) diagnosis has been fundamental in overcoming this issue. In this study, the performances of two rapid RT-qPCR assays (Real-Q Direct SARS-CoV-2 Detection Kit and Allplex™ SARS-CoV-2 fast PCR Assay) with short PCR times were comparatively evaluated using a STANDARD M nCoV Real-Time Detection Kit (STANDARD M, conventional RT-qPCR assay). All kits showed a limit of detection values (102–103 copies/reaction). The evaluation showed that the two rapid assay tests had ≥97.89% sensitivity and ≥99.51% specificity (κ = 0.98) for individual samples and ≥97.32% sensitivity and ≥97.67% specificity for pooled samples compared to STANDARD M. These results indicate that the two rapid RT-qPCR kits, which showed significant time reduction in performance, are as effective as a conventional RT-qPCR assay. They are likely to increase not only the number of tests that can be performed but also the efficiency of sustainable management of COVID-19 in the long term.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Rapid Multiplex Reverse Transcription-Quantitative Polymerase Chain Reaction Assays for SARS-CoV-2 Detection in Individual and Pooled Samples

Baek,

Park,

Lim

et al. 2023

Life

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show abstract

“…Enhanced measures may be implemented as necessary (World Health Organization 2022). Prior to the inactivation of the samples, the samples must be placed in a fully functional Class I or Class II biosafety cabinet (Pawar et al 2021). Virus isolation is too complex and has low sensitivity, and the requirement for biosafety level (BSL-3) or higher facilities makes this method impractical (Hong et al 2023).…”

Section: Laboratory Biosafetymentioning

confidence: 99%

Monkeypox Diagnosis in Clinical Settings: A Comprehensive Review of Best Laboratory Practices

Jain,

Umar,

Sayad

et al. 2024

Advances in Experimental Medicine and Biology

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An outbreak of monkeypox (Mpox) was reported in more than 40 countries in early 2022. Accurate diagnosis of Mpox can be challenging, but history, clinical findings, and laboratory diagnosis can establish the diagnosis. The pre-analytic phase of testing includes collecting, storing, and transporting specimens. It is advised to swab the lesion site with virus transport medium (VTM) containing Dacron or polyester flock swabs from two different sites. Blood, urine, and semen samples may also be used. Timely sampling is necessary to obtain a sufficient amount of virus or antibodies. The analytical phase of infectious disease control involves diagnostic tools to determine the presence of the virus. While polymerase chain reaction (PCR) is the gold standard for detecting Mpox, genome sequencing is for identifying new or modified viruses. As a complement to these methods, isothermal amplification methods have been designed. ELISAassays are also available for the determination of antibodies. Electron microscopy is another effective diagnostic method for tissue identification of the virus. Wastewater fingerprinting provides some of the most effective diagnostic methods for virus identification at the community level. The advantages and disadvantages of these methods are further discussed. Post-analytic phase requires proper interpretation of test results and the preparation of accurate patient reports that include relevant medical history, clinical guidelines, and recommendations for follow-up testing or treatment.

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“…Using a lens or mirror, the fraction of scattered light is focused on an optical photodetector, resulting in a peak proportion of the signal to particle size [1][21] [22]. The number of particles in the BSC is counted using a tool called the Particle Counter [23][24] [25].…”

Section: Introductionmentioning

confidence: 99%

Use of a Portable Particle Counter to Analyze Particle Stability Time in a Biological Safety Cabinet (BSC)

Putri

Nugraha

Yulianto

et al. 2022

j.electron.electromedical.eng.med.inform

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Biological Safety Cabinet (BSC) is a laboratory work area with air ventilation that has been engineered to protect workers working with material samples, the environment and material samples from the possible danger of contamination or causing the spread of pathogenic bacteria or viruses. The purpose of this study is to analyze the stability of the time required for the BSC to reach the condition of no particles in the BSC space. This is done by making a module using the PMS7003 sensor to detect the number of particles. This study uses the Arduino Mega system for data processing and then displays it in the form of graphs and numbers. In the condition of the number of particles of 162,965, the time required for the BSC is 29 seconds, while in the condition of the number of particles of 186,408, the time required is 38 seconds. So it is known that if the number of particles in the BSC space is more and more particles in the BSC space, the longer it takes for the BSC to reach the no-particle condition. BSC that uses a single fan blower cannot achieve a stable number of particles simultaneously.

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Selection and application of biological safety cabinets in diagnostic and research laboratories with special emphasis on COVID-19

Cited by 8 publications

References 5 publications

Evaluation of Rapid Multiplex Reverse Transcription-Quantitative Polymerase Chain Reaction Assays for SARS-CoV-2 Detection in Individual and Pooled Samples

Evaluation of Rapid Multiplex Reverse Transcription-Quantitative Polymerase Chain Reaction Assays for SARS-CoV-2 Detection in Individual and Pooled Samples

Monkeypox Diagnosis in Clinical Settings: A Comprehensive Review of Best Laboratory Practices

Use of a Portable Particle Counter to Analyze Particle Stability Time in a Biological Safety Cabinet (BSC)

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