Selection and Characterization of a Native Pycnoporus sanguineus Strain as a Lignocellulolytic Extract Producer from Submerged Cultures of Various Agroindustrial Wastes

Abstract

A native strain of Pycnoporus sanguineus (CS2) was selected because of its lignocellulolytic potential on a color-based agar plate screening system; pectinolytic and amylolytic capabilities were also assessed. The effects of different lignocellulosic substrates under submerged cultures of selected fungi on the enzymatic production of cellulases, xylanases, amylases, and laccases were evaluated. Seven different treatments, in which different combinations of wheat straw (WS), wheat bran (WB), orange peels (OP), grapefruit peels (GP), and apple peels (AP) were used, were established. Controls of 2% (w/v) carboxymethyl-cellulose (CMC) and xylan were used in a rich medium for basidiomycetes (RMB). The highest titers were achieved using OP-based cultures, with large titers of CMCases (33.5 U), avicelases (15.7 U), ß-D-glucosidase (72.9U), and xylanases (18.3 U). The best levels of amylase and laccase activity were obtained in the RMB plus CMC (RMB-CMC) (7.2 U) and in the medium OF/AP/GP (6.4 U), respectively. The relative molecular sizes of cellulase, xylanase, and amylase were 66.2, 56.5, and 90.8 kDa, respectively. Laccase and amylase had maximum activities at 60 °C whereas cellulase and hemicellulase had maximum activities at 70 °C. The optimal pH for cellulases, xylanases, and amylases was 5.0 in every case, and more than 95% activity was observed at pH 6. These results reveal some efficient operating parameters for the application of these enzymatic extracts as adjuvants to improve animal nutrition.

The effects of hydrolysed sorghum on growth performance and meat quality of rabbits

“…Trametes maxima CU1 and Pycnoporus sanguineus CS2 are 2 basidiomycetes from North-eastern Mexico with great lignocellulose potential (Hernández-Luna et al, 2008;Gutiérrez-Soto et al, 2015a), and are recognised for their production of laccases with high redox potential, as well as for the production of thermostable cellulases, xylanases and pectinases (Gutiérrez-Soto et al,2015b). The aim of the current study was to evaluate the potential use of sorghum hydrolysed by Trametes maxima CU1 and Pycnoporus sanguineus CS2 strains as a feed supplement to enhance rabbit performance and meat quality.…”

PENGARUH KONDISI KULTUR PADA AKTIVITAS SELULASE ISOLAT Pycnoporus sp. DAN Phlebiopsis sp. (EFFECTS OF CULTURE CONDITIONS ON CELLULASE ACTIVITIES PRODUCED BY Pycnoporus sp. AND Phlebiopsis sp.)

“…Sistem enzim hidrolitik terdiri sejumlah enzim, diantaranya endo-1,4-β-glukanase, selobiohidrolase (ekso-1,4-β-glukanase), β-glukosidase, dan xylanase; sedangkan sistem enzim oksidatif terdiri dari enzim lakase, mangan peroksidase dan lignin peroksidase (Gutiérrez-Soto et al, 2015). Enzimenzim penyusun selulase-kompleks memiliki peran yang sinergis dalam mengurai rantai polimer selulosa menjadi glukosa sebagai sumber karbon untuk menunjang pertumbuhan dan siklus hidup fungi tersebut (Falkoski et al, 2012).…”

“…Goyal and Soni (2011) yang meneliti optimasi produksi selulase dari isolat fungi Pleurotus florida, P. Ostreatus, dan P. sajor-caju pada media campuran Malt Ekstrak 0,5% dan CMC 1%, melaporkan bahwa kultur fungi yang diinkubasi pada pH 5,0 suhu 35-40°C menghasilkan aktivitas endo-1,4-β-glukanase dan selobiohidrolase (ekso-1,4-β-glukanase) yang paling optimal; sedangkan aktivitas β-glukosidase paling optimal diperoleh dari kultur fungi yang diinkubasi pada pH 4,5 suhu 30°C. Gutiérrez-Soto et al (2015) melaporkan bahwa Pycnoporus sanguineus yang dikultivasi pada media limbah kulit buah-buahan 81 menghasilkan titer enzim selulase paling optimal pada pH 5-6 suhu 70°C. Hal ini menunjukkan bahwa kondisi lingkungan kultur, seperti komposisi media kultivasi, pH, dan temperatur mempengaruhi aktivitas enzim yang dihasilkan.…”

“…Sejumlah isolat jamur pelapuk putih seperti Phanerochaete spp., Pleurotus spp., Trametes spp., dan Pycnoporus spp., telah diteliti dan dievaluasi kemampuannya dalam menghasilkan selulase (Goyal and Soni, 2011;Gutiérrez-Soto et al, 2015;Khalil et al, 2011;Levin, Herrmann and Papinutti, 2008;Quiroz-Castañeda et al, 2011). Pycnoporus sp.…”

SEED CULTURE CONDITIONS FOR HIGH YIELD OF CELLULOLYTIC ENZYMES PRODUCTION FROM Pycnoporus sanguineus

“…PS has a promising capacity with multi applications, so that it can be a feedstock bio refinery sector as well as for enzymes industry [5]. PS is also a good and thermostable cellulase producer that can maintain more than 90% of activities above temperature of 90 °C [6]. It can grow at moderately high temperature (37 °C ) [1].…”