Selection and characterization of in vitro–induced mutants of Dendrobium ‘Earsakul’ resistant to black rot

Khairum, Apinya; Hualsawat, S.; Chueakhunthod, Witsarut; Tharapreuksapong, Akkawat; Tomsantia, Benjawan; Yenchon, Sureerat; Papan, P.; Tantasawat, Piyada Alisha

doi:10.1007/s11627-022-10261-0

Cited by 3 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of EMS on A. odoratum is evident by the increasing concentration and duration of treatment. Similar doses were noted as effective in other studies on orchids as well [7,14]. Maximum FW of 60 ± 7 mg was attained at EMS (0.1%) and minimum 4 ± 3 mg at EMS (0.5%) for 5 days of treatment, whereas it varied from 37 ± 7 mg to 0 for 7 days of treatment [Table 1].…”

Section: Effect Of Ems Treatment On M 0 Generationsupporting

confidence: 80%

“…However, it can cause cell death either instantly or by mitotic death that occurs when the cell divides [9]. Research has shown that EMS can be used to produce mutants in a variety of crop plants [9][10][11][12] However, very few studies are available claiming the success of EMS-induced mutations in orchids [13,14]. Therefore, this study aimed to establish superior A. odoratum mutant lines in terms of morphology and osmolytes, pigments, and phenol contents.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular, morphological, and biomolecular characterization of ethyl methanesulfonate-induced mutations in Aerides odoratum, an orchid

Jyothsna,

S.,

Hallur

et al. 2024

J App Biol Biotech

View full text Add to dashboard Cite

Aerides odoratum L. is a medicinal and ornamental orchid of the Eastern Himalayas, India. Its uses in pharmacology are nought because of its rare occurrence, longer life cycle, and lack of artificial methods for large-scale propagation. Protocorms (60 days old) were treated with various concentrations of ethyl methanesulfonate ranging from 0.025% to 0.5% for different durations (2, 5, 7, and 10 days) during M0 (treatment) in vitro generation. The survival of these protocorms and the generation of shoots and roots were noted. 10–20 plantlets of each treatment showing variation in the number and weight of shoots in M0 generation (treatment generation) were maintained for three subsequent generations. M3 (third generation) axenic plantlets were hardened in the orchidarium with a 75% survival frequency. Four mutant lines (M3V1–M3V4) were established based on RAPD analysis of the leaf DNA of 300-day-old plants. M3V-1 was noted as the best among all mutant lines based on morphological analysis, whereas M3V-2 was noted as the most superior in terms of having the highest quantity of osmolytes, pigments, stomata, and phenol. M3V-3 had maximum root length, leaf breadth, and carotenoids. M3V-4 showed the viridis chlorophyll spectrum as the most distinguishing factor. Hence, this standardized protocol can be used for the genetic improvement of A. odoratum.

show abstract

Section: Effect Of Ems Treatment On M 0 Generationsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Molecular, morphological, and biomolecular characterization of ethyl methanesulfonate-induced mutations in Aerides odoratum, an orchid

Jyothsna,

S.,

Hallur

et al. 2024

J App Biol Biotech

View full text Add to dashboard Cite

show abstract

“…When compared with our previous works using ISSR markers for characterizing NaN 3 and EMSinduced Dendrobium sp. 'Earsakul' mutants from in vitro selection for black rot resistance, the extent of the found polymorphisms was lower than that found by Khairum et al (2022) and Tantasawat et al (2017) (28.02%-46.50%), but it was higher than that found by Hualsawat et al (2022) (22.67%). The polymorphism percentage is largely dependent on the types of markers and the types and number of genotypes being studied.…”

Section: Resultsmentioning

confidence: 53%

“…Sixteen Dendrobium sp. 'Earsakul' lines provided by Hualsawat et al (2022) and Khairum et al (2022) were used in our experiment: four nonmutagenized controls and 12 black rot-resistant mutants obtained with in vitro NaN 3 and EMS mutagenesis and three in vitro selection cycles with 30%-30%-40%, and 50%-50%-60% (volume/volume) culture filtrate of P. palmivora. Information about the plant materials is presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Sequence-related Amplified Polymorphism and Target Region Amplification Polymorphism Markers-based Profiling of Sodium Azide and Ethyl Methanesulfonate-derived Black Rot-resistant Dendrobium sp. ‘Earsakul’ Mutants from In Vitro Mutagenesis and Selection

Chueakhunthod,

Khairum,

Jaukwon

et al. 2023

horts

View full text Add to dashboard Cite

To reveal the applicability of sequence-related amplified polymorphism (SRAP) and target region amplification polymorphism (TRAP) markers to mutant genotyping and marker identification for resistance to black rot (Phytophthora palmivora) in orchids (Dendrobium sp. ‘Earsakul’), we fingerprinted four nonmutagenized controls and 12 black rot-resistant mutants obtained with in vitro sodium azide (NaN3) and ethyl methanesulfonate (EMS) mutagenesis and in vitro selection using culture filtrate of P. palmivora. Each of the 20 SRAP and TRAP primer combinations yielded 375 and 384 scorable DNA bands, respectively, of which 94 (24.42%) and 88 (22.91%) were polymorphic, respectively. Mantel’s test cophenetic correlation coefficients of SRAP, TRAP, and SRAP/TRAP were 0.750, 0.921, and 0.861, respectively, indicating the efficiency of these markers, especially TRAP and SRAP/TRAP, for Dendrobium sp. ‘Earsakul’ mutant characterization. Moreover, the correlations between the matrices of cophenetic correlation values for the dendrograms of SRAP with TRAP, SRAP with SRAP/TRAP, and TRAP with SRAP/TRAP were 0.399, 0.566, and 0.793, respectively, and the dendrograms based on SRAP vs. TRAP and SRAP vs. SRAP/TRAP, with lower correlations, had more variations, i.e., the number of clusters, the members of clusters, and the placement of the materials, than the ones based on TRAP vs. SRAP/TRAP. Among the three dendrograms, all nonmutagenized controls were clustered together, whereas all the highly resistant and the most resistant mutants were distributed separately as individuals. Interestingly, the four SRAP and TRAP markers were significantly associated with black rot resistance. Overall, our results will be useful for facilitating future Dendrobium sp. ‘Earsakul’ breeding programs.

show abstract

“…The development of resistant varieties is the most effective method to control black rot disease. Recently, we have developed several black rot resistant D. 'Earsakul' mutants from mutagenesis with ethyl methanesulfonate (EMS) and in vitro P. palmivora (Khairum et al, 2022). Based on genetic variation, DNA content, genome size and morphological characters, they have chanisms against P. palmivora have not yet been established.…”

Section: Introductionmentioning

confidence: 99%

Systemic induction of ß-1,3-glucanase and chitinase activities in black rot resistant mutants of Dendrobium ‘Earsakul’ in response to Phytophthora palmivora infection

Khairum,

Chueakhunthod,

Siwapitakpong

et al. 2023

Europ.J.Hortic.Sci.

View full text Add to dashboard Cite

To protect themselves against diverse pathogens, plants produce pathogenesis-related (PR) proteins as parts of constitutive and induced chemical defenses. In this study, we investigated the systemic and local induction of two PR proteins, ß-1,3response to Phytophthora palmivora infection in resistant Dendrobium and systemic induction of ß-1,3-glucanase were observed in highly resistant mutants SUT13E18301 induced chitinase activities in both distal and infected leaves. Interestingly, the up-regulated protein bands but not in the non-mutagenized controls. Some of these proteins appeared to be associated with ß-1,3-glucanase and chitinase activities, suggesting that these unique protein bands may be related to antifungal activities during the infection of P. palmivora and may be used as in orchids. These biochemical characteristics provide insight into the resistance mechanism of Dendrobium against P. palmivora and may be effectively utilized to characterize other putative D resistance resource for future breeding programs.

show abstract

Selection and characterization of in vitro–induced mutants of Dendrobium ‘Earsakul’ resistant to black rot

Cited by 3 publications

References 32 publications

Molecular, morphological, and biomolecular characterization of ethyl methanesulfonate-induced mutations in Aerides odoratum, an orchid

Molecular, morphological, and biomolecular characterization of ethyl methanesulfonate-induced mutations in Aerides odoratum, an orchid

Sequence-related Amplified Polymorphism and Target Region Amplification Polymorphism Markers-based Profiling of Sodium Azide and Ethyl Methanesulfonate-derived Black Rot-resistant Dendrobium sp. ‘Earsakul’ Mutants from In Vitro Mutagenesis and Selection

Systemic induction of ß-1,3-glucanase and chitinase activities in black rot resistant mutants of Dendrobium ‘Earsakul’ in response to Phytophthora palmivora infection

Contact Info

Product

Resources

About