Selection and Characterization of Phage Miniantibodies to Actins of Different Origin

Abstract

Single-chain miniantibodies (scFv's) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv's (containing 2x10(8) independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4x10(7) and 1.2x10(6) M(-1) were chosen. The isolated miniantibodies could specifically detect various plant and animal actins.

Quantitative cell bioimaging using gold‐nanoshell conjugates and phage antibodies

“…Phabs (also termed recombinant antibodies) against SPEV cells were obtained by using a combinatorial sheep phage display library, kindly provided by Prof. W. Harris (University of Aberdeen, UK) [34]. To obtain specific phages, we performed three selection rounds as described previously [35]. Put at its simplest, phage display is the expression of singlechain Fv (scFv) antibody fragments at the surface of phage particles.…”