Selection and electrochemical-sensor application of an DNA-aptamer for methyl parathion detection

Recent Progress in Single-Nucleotide Polymorphism Biosensors

“…Biosensing methods based on electrochemical transduction mechanisms have been reported to be sensitive, selective, rapid, and amenable to miniaturization and experimental convenience [ 89 , 90 , 91 , 92 , 93 , 94 ]. A variety of strategies aiming at improving the target recognition and signal transduction performance have been developed [ 95 , 96 , 97 , 98 , 99 ]. Owing to the features of enzyme-free, LNA-integrated, and toehold-mediated SDR techniques, Gao et al developed a reusable DNA sensor for SNP detection.…”

An electrochemical aptasensor based on silver-thiolated graphene for highly sensitive detection of Pb2+

“…15,16 AuNPs have good chemical stability, electronic properties, remarkable catalytic activity and excellent biocompatibility; therefore, their modied nanocomposites have been widely used in biosensing applications. [17][18][19] An aptamer [20][21][22] is a small fragment of DNA or RNA that specically recognizes the corresponding ligand with high specicity and affinity for its target ion or molecule, now widely used for sensor construction. Mamta Kushwah et al 23 constructed a rGO/AuNPs/ssDNA-based electrochemical aptasensor for Pb 2+ , which has been successfully used for the detection of Pb 2+ in environmental water samples.…”

“…An aptamer 20–22 is a small fragment of DNA or RNA that specifically recognizes the corresponding ligand with high specificity and affinity for its target ion or molecule, now widely used for sensor construction. Mamta Kushwah et al 23 constructed a rGO/AuNPs/ssDNA-based electrochemical aptasensor for Pb 2+ , which has been successfully used for the detection of Pb 2+ in environmental water samples.…”

Ultrasensitive Electrochemical Aptasensing of Malathion Based on Hydroxylated Black Phosphorus/Poly-L-Lysine Composite

“…However, there are two lone pair electrons in each phosphorus atom [18], so it is highly sensitive to oxygen and humidity [19], easily oxidized and degraded by air, and its stability is poor [20]. Aptamers are "chemical antibodies", which also have some advantages that antibodies do not have, such as easy chemical synthesis [21], non-immunogenicity, small size, good biochemical stability [22], and so on. The targets for aptamers include proteins [23], viruses, small molecular organics [24][25][26][27], and living cells, which can bind to a wide range of substances.…”