Selection and evolution of resistance to antimicrobial drugs

Hughes, Diarmaid

doi:10.1002/iub.1278

Cited by 38 publications

(20 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A phenomenon of selection of streptomycin‐resistant strains could have influenced the higher frequency and persistence of strains of ST32 between Chilean Cmm strains studied. Hughes () discussed how bacteria respond to the selective pressure upon antibiotic exposure. Antibiotic usage over many years has selected a high frequency of resistant strains among clinically relevant bacterial pathogens, and once resistant strains with high fitness are established in a bacterial community, it is very difficult to reduce their resistance frequency.…”

Section: Discussionmentioning

confidence: 99%

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

et al. 2018

View full text Add to dashboard Cite

Streptomycin has been used for decades in Chile to control Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of tomato bacterial canker. The aim of this work was to evaluate streptomycin resistance and to analyse the presence of resistance‐related genes in Cmm strains from Chile. A collection of 25 Cmm strains isolated from different localities in central Chile between 1996 and 2015 was analysed. Minimum inhibitory concentration (MIC) of streptomycin was determined. A search of streptomycin resistance‐related genes was carried out in Cmm genomes, and the presence of these genes was studied in all Chilean strains using PCR and sequencing techniques. MIC results showed that four of 25 strains were highly sensitive to streptomycin, with MIC values <2 μg mL−1. The remaining 21 strains possessed MIC of streptomycin ≥100 μg mL−1. The strB gene, encoding an aminoglycoside 6‐phosphotransferase that inactivates streptomycin, was detected in all Chilean strains, including sensitive and resistant strains. In the 21 resistant strains, a mutation in codon 43 of the rpsL gene was determined, conferring high streptomycin resistance. Interestingly, the four streptomycin‐sensitive Cmm strains did not possess this mutation. This study proposes that the continuous use of streptomycin leads to emergence of resistant Cmm strains, challenging researchers to look for novel alternatives to control this plant pathogenic bacterium.

show abstract

Section: Discussionmentioning

confidence: 99%

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Antimicrobial resistance is one of the greatest threats to global public health and the World Health Organisation warns against a return to a pre-antibiotic era [1]. Higher prevalence of resistance among human pathogens increases the risk of uncontainable infections, prolonged illness and hospital stay, increased mortality, and consequently increased health care costs [2]. Antibiotic use is the main driver of antibiotic resistance, why addressing the excessive and inappropriate use of antibiotics is essential [3].…”

Section: Introductionmentioning

confidence: 99%

Acute lower respiratory tract infections: Symptoms, findings and management in Danish general practice

Hansen

Lykkegaard

Thomsen

et al. 2019

European Journal of General Practice

View full text Add to dashboard Cite

show abstract

“…Further, it is important to consider potential alterations in the fitness of MDROs in causing persistent colonization or infection. Although mutations that lead to antibiotic resistance may result in reduced fitness, studies have shown that additional mutations over time may compensate for these fitness costs and that MDROs can subsequently become even more virulent even in settings free of antimicrobials (12)(13)(14)(15).…”

mentioning

confidence: 99%

Fecal Microbiota Transplantation and Successful Resolution of Multidrug-Resistant-Organism Colonization

2015

View full text Add to dashboard Cite

cWe report a case in which fecal microbiota transplantation (FMT) utilized for relapsing Clostridium difficile colitis successfully eradicated colonization with several multidrug-resistant organisms (MDROs). FMT may have an additive benefit of reducing MDRO carriage and should be further investigated as a potential measure to eradicate additional potentially virulent organisms beyond C. difficile. CASE REPORTA 66-year-old male was admitted to Scripps Mercy Hospital in June of 2012 for debridement of a large sacral wound. His past medical history was significant for a spinal epidural abscess in March of 2011 that resulted in C4-level spinal cord injury (quadriplegia). His condition required residence in a skilled nursing facility and placement of a tracheostomy and a feeding tube and chronic Foley catheterization. While at the facility, he was found to be colonized by multiple multidrug-resistant (MDR) organisms (MDROs), including carbapenem-resistant Enterobacteriaceae (CRE), methicillin-resistant Staphylococcus aureus (MRSA), and MDR Acinetobacter baumannii, and developed sepsis on a monthly basis during the 6 months preceding admission. Three days prior to admission, he was diagnosed with a CRE Klebsiella pneumoniae urinary tract infection (UTI).As a result of his ventilator-dependent condition and complex medical needs, he was admitted to the intensive care unit (ICU) at our facility. The sacral wound was treated with debridement and flap placement as well as a diverting colostomy. He was diagnosed with Clostridium difficile colitis during the first week of admission and treated with oral vancomycin (250 mg orally [p.o.] via a feeding tube 4 times/day). In addition, during the first 15 weeks of hospitalization, a variety of MDROs were isolated, including carbapenem-resistant (CR) Pseudomonas aeruginosa (respiratory tract), MDR Acinetobacter baumannii (wound and respiratory tract), CRE Klebsiella pneumoniae (wound), vancomycin-resistant Enterococcus faecalis (VRE) (wound), and MRSA (respiratory tract, urine, and abdominal fluid) (Fig. 1A). The patient received antibiotic courses for these infections, but these were limited to short courses due to concurrent C. difficile colitis and MDROs that were resistant to all or the majority of antibiotics.Despite continual oral vancomycin therapy for C. difficile colitis over the first 3.5 months of hospitalization, multiple episodes of relapsing C. difficile colitis occurred when vancomycin therapy was tapered. Hence, in September 2012, voluntary informed consent was obtained for a FMT. A stool donation was obtained from his sister, who underwent predonation blood and stool screening that excluded a variety of bacterial, parasitic, and viral pathogens (i.e., HIV, hepatitis A, B, and C virus, and Treponema pallidum [syphilis], plus stool examination for ova and parasites [O&P], C. difficile, and enteric pathogens). Vancomycin treatment was discontinued on the day prior to FMT, and the donor stool (480 ml) was transferred to the patient via a colonoscope. He tolerated the...

show abstract

Selection and evolution of resistance to antimicrobial drugs

Cited by 38 publications

References 60 publications

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

Acute lower respiratory tract infections: Symptoms, findings and management in Danish general practice

Fecal Microbiota Transplantation and Successful Resolution of Multidrug-Resistant-Organism Colonization

Contact Info

Product

Resources

About