Selection and mechanism exploration for salt-tolerant genes in tomato

Kou, Xiaohong; Chen, Xiuyu; Mao, Cuiyu; He, Yulong; Feng, Yang; Wu, Caie; Xue, Zhaohui

doi:10.1080/14620316.2018.1486739

Cited by 5 publications

(5 citation statements)

References 46 publications

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“…Five candidate genes for salt tolerance in tomato were chosen based on literature. The genes HKT1;1 , WRKY8 , SOS1 and SOS2 were previously shown to be associated with salt tolerance in tomato [ 40 , 73 – 76 ]. The gene CESA2 was also included as it showed a high homology to AtCESA6 , which is important to salt tolerance in Arabidopsis thaliana [ 77 ].…”

Section: Methodsmentioning

confidence: 99%

Synthetic bacterial community derived from a desert rhizosphere confers salt stress resilience to tomato in the presence of a soil microbiome

et al. 2022

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The root bacterial microbiome is important for the general health of the plant. Additionally, it can enhance tolerance to abiotic stresses, exemplified by plant species found in extreme ecological niches like deserts. These complex microbe-plant interactions can be simplified by constructing synthetic bacterial communities or SynComs from the root microbiome. Furthermore, SynComs can be applied as biocontrol agents to protect crops against abiotic stresses such as high salinity. However, there is little knowledge on the design of a SynCom that offers a consistent protection against salt stress for plants growing in a natural and, therefore, non-sterile soil which is more realistic to an agricultural setting. Here we show that a SynCom of five bacterial strains, originating from the root of the desert plant Indigofera argentea, protected tomato plants growing in a non-sterile substrate against a high salt stress. This phenotype correlated with the differential expression of salt stress related genes and ion accumulation in tomato. Quantification of the SynCom strains indicated a low penetrance into the natural soil used as the non-sterile substrate. Our results demonstrate how a desert microbiome could be engineered into a simplified SynCom that protected tomato plants growing in a natural soil against an abiotic stress.

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Section: Methodsmentioning

confidence: 99%

Synthetic bacterial community derived from a desert rhizosphere confers salt stress resilience to tomato in the presence of a soil microbiome

et al. 2022

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“…PCR amplification and quantitative real-time PCR (qRT-PCR) were performed using S1000 thermal cycler (BIO-RAD, USA) and LightCycler96 (Roche, Basel, Switzerland), respectively. β-Tublin was selected as the internal reference gene [26].…”

Section: Determination Of the Genes Expression Related To Sugar Metabmentioning

confidence: 99%

Effects of Exogenous Trehalose on the Metabolism of Sugar and Abscisic Acid in Tomato Seedlings Under Salt Stress

Feng

Chen

et al. 2019

Trans. Tianjin Univ.

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Salt stress affects the growth and development of plants, which results in a decrease in crop quality and yield. In this study, we used tomato seedlings treated with salt and trehalose as experimental materials and analyzed them using the technique for order preference by similarity to ideal solution analysis to select the optimal trehalose concentration for treatment. We also determined the contents of sugar and abscisic acid (ABA) and detected the expression of genes involved in the metabolism of sugar and ABA by quantitative real-time PCR. Results showed that the optimal trehalose concentration was 2 mmol/L for tomato seedlings under salt stress. Exogenous trehalose decreased the starch content and increased the soluble sugar content by affecting the expression of genes related to the metabolism of starch and soluble sugar. Exogenous trehalose altered the accumulation and distribution of sugar by inducing the upregulation of sugar transporter genes. Furthermore, trehalose increased the ABA content to induce salt stress response by regulating the expression of genes related to the synthesis and metabolism of ABA. In conclusion, trehalose can effectively alleviate salt stress and enhance salt tolerance of tomato. These findings provide a novel perspective and a better resource to investigate the salt tolerance mechanism and a new method for alleviating salt stress in tomato.

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“…In response to abiotic stresses such as drought, enzymatic and catalytic activities in plants, such as superoxide dismutase, are altered, which may lead to increased oxidative stress and affect the organism’s growth and development. Eight genes were selected for the validation of the transcriptome analysis’ results ( Figure 9 D), among which GH3.1 ( Potri.001G298300 ), GH3.6 ( Potri.014G136800 ), MADS-box18 ( Potri.003G170000 ), and MADS-box27 ( Potri.009G079000 ) may be involved in root growth and development [ 47 , 48 ], and RGLG2 ( Potri.006G002600 ), PP2C44 ( Potri.008G168400 ), MYB4 ( Potri.002G038500 ), and ASR3 ( Potri.005G193600 ) may play important roles in abiotic stress [ 49 , 50 , 51 , 52 ]. The results of the qRT-PCR analysis were highly consistent with the RNA-Seq expression trends, indicating that the DEGs identified through the RNA-Seq screening were reliable.…”

Section: Resultsmentioning

confidence: 99%

PtrABR1 Increases Tolerance to Drought Stress by Enhancing Lateral Root Formation in Populus trichocarpa

Sun,

Dong,

Song

2023

IJMS

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Roots are the main organ for water uptake and the earliest part of a plant’s response to drought, making them of great importance to our understanding of the root system’s response to drought. However, little is known about the underlying molecular mechanisms that control root responses to drought stress. Here, we identified and functionally characterized the AP2/ERF family transcription factor (TF) PtrABR1 and the upstream target gene zinc-finger protein TF PtrYY1, which respond to drought stress by promoting the growth and development of lateral roots in Populus trichocarpa. A root-specific induction of PtrABR1 under drought stress was explored. The overexpression of PtrABR1 (PtrABR1-OE) promoted root growth and development, thereby increasing tolerance to drought stress. In addition, PtrYY1 is directly bound to the promoter of PtrABR1 under drought stress, and the overexpression of PtrYY1 (PtrYY1-OE) promoted lateral root growth and development and increased tolerance to drought stress. An RNA-seq analysis of PtrABR1-OE with wild-type (WT) poplar identified PtrGH3.6 and PtrPP2C44, which share the same pattern of expression changes as PtrABR1. A qRT-PCR and cis-element analysis further suggested that PtrGH3.6 and PtrPP2C44 may act as potential downstream targets of PtrABR1 genes in the root response pathway to drought stress. In conclusion, these results reveal a novel drought regulatory pathway in which PtrABR1 regulates the network through the upstream target gene PtrYY1 and the potential downstream target genes PtrGH3.6 and PtrPP2C44, thereby promoting root growth and development and improving tolerance to drought stress.

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Selection and mechanism exploration for salt-tolerant genes in tomato

Cited by 5 publications

References 46 publications

Synthetic bacterial community derived from a desert rhizosphere confers salt stress resilience to tomato in the presence of a soil microbiome

Synthetic bacterial community derived from a desert rhizosphere confers salt stress resilience to tomato in the presence of a soil microbiome

Effects of Exogenous Trehalose on the Metabolism of Sugar and Abscisic Acid in Tomato Seedlings Under Salt Stress

PtrABR1 Increases Tolerance to Drought Stress by Enhancing Lateral Root Formation in Populus trichocarpa

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