Selection and Preparation of Explants for the Clonal Propagation of Horticultural Plants in Plant Factory Systems

Tigrel, Ahmet; Arslan, Merve; Arıcı, Beyza; Yücesan, Buhara

doi:10.1007/978-981-19-0055-6_2

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…The explants consisted of trimmed branches with sprouting fresh shoots. Meristematic nodal explants were cut into pieces measuring 2.5 cm in length and were subjected to surface sterilization following the protocol described in Tigrel et al (2022). Brie y, the fresh shoot explants, after sprouting from the nodes on each branch, were immersed in a commercial bleach solution containing < 5% sodium hypochlorite for 20 minutes, followed by a 1-minute dip in 70% ethanol (v/v).…”

Section: Selection Of Plant Materialsmentioning

confidence: 99%

Effects of Sucrose and 6-Benzylaminopurine Concentrations on Shoot Regeneration and Vitrification in Aronia melanocarpa: Insights for Plant Tissue Culture Systems

Bayhan,

Yucesan

2023

Preprint

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This study focuses on investigating the effects of sucrose and 6-Benzylaminopurine (BAP) concentrations on shoot regeneration and the development of vitrification in Aronia melanocarpa. Initially, the presence of BAP, in combination with varying sucrose concentrations, leads to a substantial increase in shoot number. The highest shoot formation is observed in the MS medium containing 3% sucrose and 5.0 mg/L BAP. Furthermore, sucrose concentration plays a crucial role in shoot growth, with higher concentrations promoting more extensive shoot development. However, when sucrose levels were at 3% combined with higher BAP concentrations (1.0 or 2.5 mg/L), an increased incidence of vitrification was observed over time. Interestingly, lower sucrose concentrations (1% or 2%) combined with 0.5 mg/L or 2.5 mg/L BAP initially delayed vitrification but eventually led to its occurrence. Microscopic analysis of leaf samples with varying levels of vitrification indicates significant differences in stoma characteristics, such as density and size, further confirming the detrimental impact of vitrification on cellular structures and physiological processes. The recovery of vitrified plants was evaluated using different growth media combinations. The absence of BAP in the medium led to higher recovery percentages without necrosis, while the addition of 0.5 mg/L BAP promoted shoot growth but potentially inhibited root development. During acclimatization, vitrified plants exhibited stunted shoot growth, shorter and narrower leaves, reduced root numbers and lengths, and decreased survival rates, particularly under lower humidity conditions. The cultivation period required for the recovery of the crop was determined to be six weeks under controlled greenhouse conditions.

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Section: Selection Of Plant Materialsmentioning

confidence: 99%

Effects of Sucrose and 6-Benzylaminopurine Concentrations on Shoot Regeneration and Vitrification in Aronia melanocarpa: Insights for Plant Tissue Culture Systems

Bayhan,

Yucesan

2023

Preprint

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Sustainable Management of Soilborne Diseases of Horticultural Crops

Visen,

Thakur,

Atluri

2024

Advances in Postharvest and Analytical Technology of Horticulture Crops

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Preserving Nature's Balance in the Intelligent Systems Era

Garole,

Reddy,

Lippert

2024

Advances in Mechatronics and Mechanical Engineering

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The proposed chapter provides a thorough examination of related ideas, beginning with a basic knowledge of ecological systems and the significance of biodiversity, explores the patterns and processes of biodiversity, including the measurements and methods for quantifying it along with the variables, like habitat heterogeneity and latitude, that affect its distribution. The paper then explores the patterns and processes of biodiversity, including the measurements and methods for quantifying it along with the variables, like habitat heterogeneity and latitude, that affect its distribution.. The study also outlines important risks to biodiversity, highlighting the significant contribution of human activity to biodiversity loss. The study examines potential future paths for biodiversity conservation and research, taking into account new developments in technology and fashion while recognizing the continuous difficulties in maintaining biodiversity in the face of global change.

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Selection and Preparation of Explants for the Clonal Propagation of Horticultural Plants in Plant Factory Systems

Cited by 3 publications

References 45 publications

Effects of Sucrose and 6-Benzylaminopurine Concentrations on Shoot Regeneration and Vitrification in Aronia melanocarpa: Insights for Plant Tissue Culture Systems

Effects of Sucrose and 6-Benzylaminopurine Concentrations on Shoot Regeneration and Vitrification in Aronia melanocarpa: Insights for Plant Tissue Culture Systems

Sustainable Management of Soilborne Diseases of Horticultural Crops

Preserving Nature's Balance in the Intelligent Systems Era

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