Selection and properties of spontaneous mutants ofListeria monocytogenes ATCC 15313 resistant to different bacteriocins produced by lactic acid bacteria strains

Rekhif, N.; Atrih, Abdelmadjid; Lefèbvre, G.

doi:10.1007/bf01575967

Cited by 93 publications

(90 citation statements)

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“…Moreover, some listerias could become immune probably through the outgrowth of a spontaneous mutant population resistant to nisin (Hanlin et al Individual lactic acid bacteria and cheese starter cultures 1993). Although the majority of the initial target cells are inhibited by nisin, some cells can escape and regrow after an apparent lag time (Rekhif et al 1994). The mechanism of nisin resistance is attributed to changes in the cell membrane (fatty acid composition and fluidity) and S-layer (Mantovani & Russell 2001).…”

Section: Resultsmentioning

confidence: 99%

“…The big concern in this area is the danger of development of highly tolerant and/or resistant strains. It has been observed that listerias develop tolerance towards nisin and pediocin-like substance (PLS) in laboratory media at relatively high frequency (Rekhif et al 1994). In addition, the resistance to a bacteriocin may extend to other bacteriocins within the same class or even in other classes and thus create multi-resistance listeria strains (Naghmouchi et al 2007).…”

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confidence: 99%

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Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources

Složilová¹,

Purkrtová²,

Kosová³

et al. 2014

Czech J. Food Sci.

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Abstract:Složilová I., Purkrtová S., Kosová M., Mihulová M., Šviráková E., Demnerová K. (2014): Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources. Czech J. Food Sci., 32: 145-151.Eight individual bacteriocin-producing lactic acid bacteria (LAB) strains and three bacteriocin-non-producing cheese starter cultures were evaluated for their ability to inhibit the growth of six Listeria monocytogenes strains, originating from the guinea-pig lymph nodes, raw cow milk, and manufacturing dairy equipment. Results showed that either live cells or cell-free neutralised supernatant (CFNS) and/or heated CFNS of six individual LAB strains (Lcc. lactis subsp. lactis CCDM 416 and NIZO R5, Lbc. plantarum HV 11 and DC 1246, P. acidilactici HV 12, and Ent. mundtii CCM 1282) and one starter culture (DELVO-ADD ® 100-X DSF) were effective in the suppression of at least one listeria strain. Neither any individual LAB strain nor starter culture was antagonistic toward all studied L. monocytogenes strains, indicating diverse sensitivity/resistance among L. monocytogenes strains to antimicrobial compounds of LAB. The significant susceptibility of listerias isolated from raw milk and dairy equipment together with the strong antilisterial activity of DELVO-ADD ® 100-X DSF could be applied in dairy technology, where commonly used starter cultures could play both the biopreservative and fermentation role.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources

Složilová¹,

Purkrtová²,

Kosová³

et al. 2014

Czech J. Food Sci.

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“…1996 ; Maisnier-Patin and Richard 1996). The mechanism of resistance to class IIa bacteriocins, on the other hand, Strains and crude bacteriocin preparation remains poorly understood, but is reportedly a stable phenomenon (Rekhif et al 1994 After an 18 h growth cycle, equivalent aliquots of the for bacteriocin production in this study. All strains were grown for 48 h in de Man, Rogosa and Sharpe (MRS) broth co-cultures were transferred to fresh medium, to a total of 12 successive transfers.…”

mentioning

confidence: 95%

“…The frequency of the spontaneous resistant phenotype however, was lower than the 10 −3 -10 −4 reported by a BHI bacteriocin-free control series. Plates were incubated for 48 h and the relative numbers of cfu on bacteriocin- Rekhif et al (1994) and may be attributed to strain-specific variations in spontaneous mutation rate. In contrast to the containing and control plates determined.…”

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confidence: 99%

Fitness costs associated with class IIa bacteriocin resistance in Listeria monocytogenes B73

Dykes¹,

Hastings²

1998

Letters in Applied Microbiology

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I NG S. 1998. In order to assess the potential for the spread of class IIa bacteriocin resistance in natural populations of Listeria monocytogenes, the fitness costs associated with resistance to leucocins A, B and E and sakacin A in L. monocytogenes B73 in the absence of bacteriocin were examined. The resistant phenotype had a lower growth rate (and thus relative fitness) than the sensitive phenotype in monoculture experiments. Furthermore, resistant phenotypes were unable to invade populations of the sensitive strain, even at frequencies of 10 −1 or higher, when grown in co-culture. These results held true for resistant strains that had been exposed to bacteriocin for 25 successive growth cycles. It was concluded that the class IIa bacteriocin-resistant phenotype of L. monocytogenes B73 is unlikely to become stable in natural populations based on this evidence. Due to the possibility of variations in the frequencies of spontaneous mutation and fitness among Listeria strains, however, the extrapolation of these results to the species as a whole should not be made. INTRODUCTIONthe class IIa group is apparent, while bacteriocins belonging to other classes are not protected against (Rekhif et al. 1994). Bacteriocins are proteinaceous anti-competitor toxins, pro-The ecology of resistance to antimicrobial compounds has duced by bacteria, that act against the same or closely related been investigated with respect to many commonly used traspecies (Jack et al. 1995 ; Nes et al. 1996). The class II ditional antibiotics, such as tetracycline, because of the medibacteriocins are a subset of these compounds, which are cal significance of the spread of such resistance (Johnson and characteristically small, heat-stable, cationic peptides, proAdams 1992). Little, however, is known about the ecology of duced by lactic acid bacteria (Klaenhammer 1993). Class IIa resistance to bacteriocins and other antimicrobial peptides bacteriocins, such as the leucocins produced by members of (Frank 1994 ; Dykes 1995). With an increased interest in the the genus Leuconostoc, exhibit inhibitory activity against the commercial application of bacteriocins as biopreservatives (de important foodborne pathogen Listeria monocytogenes Vuyst and Vandamme 1994), investigations into the ecology (Hastings et al. 1991 ;Klaenhammer 1993 ; Felix et al. 1994).of resistance to these compounds is important to assess the The development of spontaneous L. monocytogenes mutants, potential for spread of resistance in natural populations. In resistant to class IIa bacteriocins such as mesenterocin 52 and this study the fitness costs associated with class IIa bacteriocin pediocin AcH, have been reported (Larsen and No Ârrung resistance in L. monocytogenes was investigated, along with 1993 ; Noerlis and Ray 1994 ;Rekhif et al. 1994). The mechimplications this has for the spread of resistance in popuanism of resistance to bacteriocins such as nisin, which lations of this pathogen. belongs to the class I bacteriocins or lantibiotics, have been investigated widely and...

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“…The nisin resistant strain of L. monocytogenes ATCC 700302 was observed to be showing cross resistance to the class IIa bacteriocin pediocin PA-1 and the class IV leuconocin S (Crandall and Montville 1998). L. monocytogenes mutants resistant to mesenterocin 52, curvaticin 13, and plantaricin were also reported to be cross-resistant to the other bacteriocins (Rekhif et al 1994). In addition, piscicolin 126-resistant mutants of L. monocytogenes which emerged in cheese made from milk containing the bacteriocin were also resistant to pediocin P02 (Ukuku and Shelef 1997).…”

Section: Bacteriocins Cross Resistancementioning

confidence: 99%

Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

et al. 2011

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The bacteriocin susceptibility of Listeria monocytogenes MTCC 657, Enterococcus faecium DSMZ 20477, E. faecium VRE, and E. faecalis ATCC 29212 and their corresponding bacteriocin resistant variants was assessed. The single and combined effect of nisin and pediocin 34 and enterocin FH99 bacteriocins produced by Pediococcus pentosaceus 34, and E. faecium FH99, respectively, was determined. Pediocin34 proved to be more effective in inhibiting L. monocytogenes MTCC 657. A greater antibacterial effect was observed against E. faecium DSMZ 20477 and E. faecium (VRE) when the a combination of nisin, pediocin 34 and enterocin FH99 were used whereas in case of L. monocytogenes MTCC 657 a combination of pediocin 34 and enterocin FH99 was more effective in reducing the survival of pathogen. Bacteriocin cross-resistance and the antibiotic susceptibility of wild type and their corresponding resistant variants were assessed and results showed that resistance to a bacteriocin may extend to other bacteriocins within the same class and also the acquired resistance to bacteriocins can modify the antibiotic susceptibility/resistance profile of the bacterial species used in the study. According to the hydrophobicity nisin resistant variant of L. monocytogenes was more hydrophobic (p<0.001), whereas the pediocin 34 and enterocin FH99 resistant variants were less hydrophobic than the wild type strain. Nisin, pediocin 34 and enterocin FH99 resistant variants of E. faecium DSMZ 20477 and E. faecium VRE were less hydrophobic than their wild type counterparts. Nisin resistant E. faecalis ATCC 29212 was less hydrophobic than its wild type counterpart.

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Selection and properties of spontaneous mutants ofListeria monocytogenes ATCC 15313 resistant to different bacteriocins produced by lactic acid bacteria strains

Cited by 93 publications

References 13 publications

Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources

Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources

Fitness costs associated with class IIa bacteriocin resistance in Listeria monocytogenes B73

Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

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