Selection for Monogenic Pest Resistance Traits with Coupling‐ and Repulsion‐Phase RAPD Markers

Haley, Scott D.; Afanador, L.; Kelly, James D.

doi:10.2135/cropsci1994.0011183x003400040041x

Cited by 81 publications

(47 citation statements)

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“…A number of RAPD markers: OAD19 690 linked (1.9 cM) in coupling and OS13 660 linked (7.1 cM) in repulsion to the bc-3 gene have been identified (Haley et al, 1994a). In addition, Johnson et al (1997) developed SCAR markers from the OC11 350/420 (ROC11) and OC20 460 RAPD markers linked to the bc-3 gene to improve their utilization.…”

Section: Introductionmentioning

confidence: 99%

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

et al. 2005

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SummaryNecrotic strains of bean common mosaic potyviruses are becoming increasingly problematic in bean growing areas of Africa and Europe. Pyramiding epistatic resistance genes provides the most effective long-term strategy for disease control against all known strains of the virus. Indirect selection using tightly linked markers should facilitate the breeding of desired epistatic resistance gene combinations. In common bean, the most effective strategy for broad spectrum control of the bean common mosaic potyviruses is to combine I and bc-3 genes. We describe the use of near-isogenic lines and segregating populations from different gene pools combined with bulked segregant analysis to identify markers tightly linked with the recessive bc-3 gene that conditions resistance to all strains of bean common mosaic necrosis virus. We identified a RAPD marker, OG6 595 , linked at 3.7 cM from the bc-3, and the marker was used to confirm the location of bc-3 gene on bean linkage group B6. A codominant AFLP marker, E ACA M CGG -169/172 was identified and linked at 3.5 cM from the bc-3 and the AFLP and OG6 595 markers flanked the bc-3 gene. The AFLP marker was converted to the STS marker SE ACA M CGG -134/137 which showed co-segregation with the original AFLP marker. The 134 bp fragment associated with resistance was linked with the bc-3 gene present in a diverse group of bean genotypes except in two kidney bean lines. The OG6 595 marker mapped on B6 supported independence of bc-3 from the I gene located on B2, which provides the opportunity to readily combine both genes in a single bean cultivar for broad spectrum resistance to bean common mosaic potyviruses.

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Section: Introductionmentioning

confidence: 99%

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

et al. 2005

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“…The coupling and repulsion markers can be used together as a codominant pair and will be equally informative as codominant markers in detecting heterozygotes in segregating populations (Haley et al, 1994;Johnson et al, 1995). AFLP combines principles of both RFLP and RAPD and involves four steps: a) restriction digestion of genomic DNA and ligation of oligonucleotide adapters, b) pre-selective amplification of the restricted fragments with primers specific to adapters, c) selective amplification of a set of restriction fragments, and d) gel electrophoretic separation of the amplified fragments (Vos et al, 1995).…”

Section: Genetic Markersmentioning

confidence: 99%

Chickpea Improvement: Role of Wild Species and Genetic Markers

Singh

Sharma

Varshney

et al. 2008

Biotechnology and Genetic Engineering Reviews

111

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Chickpea is an important grain legume of the semi arid tropics and warm temperate zones, and forms one of the major components of human diet. However, a narrow genetic base of cultivated chickpea (Cicer arietinum L.) has hindered the progress in realizing high yield gains in breeding programs. Furthermore, various abiotic and biotic stresses are the major bottlenecks for increasing chickpea productivity. Systematic collection and evaluation of wild species for useful traits has revealed presence of a diverse gene pool for tolerance to the biotic and abiotic stresses. Relationships among the species of genus Cicer are presented based on crossability, karyotype and molecular markers. The reproductive barriers encountered during interspecific hybridization are also examined. Recent information on genetic linkage maps, comparison of isozymes

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“…Gene pyramiding is an alternative strategy for the development of varieties with durable resistance, but traditional breeding procedures are inefficient for such purpose due to the difficulties brought about by the need for multiple inoculations (Michelmore, 1995). Pyramiding of resistance genes assisted by molecular markers has been proposed as an alternative solution for this type of problem (Haley et al, 1993(Haley et al, , 1994Johnson and Gepts, 1994;Johnson et al, 1995;Young and Kelly, 1996a).…”

Section: Introductionmentioning

confidence: 99%

“…Random amplified polymorphic DNA (RAPD) markers (Williams et al, 1990) have been successfully used in common bean breeding programs to tag resistance genes for several important diseases (Haley et al, 1993(Haley et al, , 1994Johnson and Gepts, 1994;Johnson et al, 1995;Young and Kelly, 1996a;Alzate-Marin et al, 1999a). This technique is being used in our common bean breeding program to facilitate the development of isolines containing disease resistance genes of interest.…”

Section: Introductionmentioning

confidence: 99%

Identification of a RAPD marker linked to the Co-6 anthracnose resistant gene in common bean cultivar AB 136

et al. 2000

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The pathogenic variability of the fungus Colletotrichum lindemuthianum represents an obstacle for the creation of resistant common bean (Phaseolus vulgaris L.) varieties. Gene pyramiding is an alternative strategy for the development of varieties with durable resistance. RAPD markers have been proposed as a means to facilitate pyramiding of resistance genes without the need for multiple inoculations of the pathogens. The main aims of this work were to define the inheritance pattern of resistance present in common bean cultivar AB 136 in segregating populations derived from crosses with cultivar Rudá (susceptible to most C. lindemuthianum races) and to identify RAPD markers linked to anthracnose resistance. The two progenitors, populations F1 and F2, F2:3 families and backcross-derived plants were inoculated with race 89 of C. lindemuthianum under environmentally controlled greenhouse conditions. The results indicate that a single dominant gene, Co-6, controls common bean resistance to this race, giving a segregation ratio between resistant and susceptible plants of 3:1 in the F2, 1:0 in the backcrosses to AB 136 and 1:1 in the backcross to Rudá. The segregation ratio of F2:3 families derived from F2 resistant plants was 1:2 (homozygous to heterozygous resistant). Molecular marker analyses in the F2 population identified a DNA band of approximately 940 base pairs (OPAZ20(940)), linked in coupling phase at 7.1 cM of the Co-6 gene. This marker is being used in our backcross breeding program to develop Rudá-derived common bean cultivars resistant to anthracnose and adapted to central Brazil.
A antracnose do feijoeiro, causada por Colletotrichum lindemuthianum, é uma doença que ocasiona severas perdas, estando amplamente distribuída no Brasil. Trabalhos anteriores têm demonstrado que o cultivar AB 136, possuidor do gene Co-6, é uma importante fonte de resistência à antracnose no Brasil, uma vez que todos os patótipos identificados no país até o momento são incompatíveis com esse cultivar. O objetivo deste trabalho foi identificar um marcador RAPD ligado ao gene de resistência Co-6 presente no cultivar AB 136. Para este fim, foi utilizada uma população do cruzamento entre o progenitor Rudá, suscetível à raça 89 de C. lindemuthianum, e o cultivar AB 136. Nas populações F2 e F3 deste cruzamento observou-se uma segregação de 3 plantas resistentes para 1 suscetível, e 1 planta homozigota para 2 heterozigotas, respectivamente, sugerindo que a resistência é determinada pela ação de um único gene. Na população F2, foi identificado um marcador, de aproximadamente 940 pb (OPAZ20), ligado em fase de acoplamento a 7,1 cM do gene Co-6. Este marcador está sendo utilizado em nosso programa de melhoramento para desenvolver cultivares de feijoeiro derivados de Rudá resistentes à antracnose e adaptados ao Brasil Central

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Selection for Monogenic Pest Resistance Traits with Coupling‐ and Repulsion‐Phase RAPD Markers

Cited by 81 publications

References 0 publications

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

Chickpea Improvement: Role of Wild Species and Genetic Markers

Identification of a RAPD marker linked to the Co-6 anthracnose resistant gene in common bean cultivar AB 136

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