Selection, Formulation, and Field Evaluation of Bacillus amyloliquefaciens PMB01 for Its Application to Manage Tomato Bacterial Wilt Disease

Chou, Hau-Ping; Huang, Yi-Chiao; Lin, Yi-Hsien; Deng, Wen-Ling

doi:10.3390/agriculture12101714

Cited by 7 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Toluquinol formulations were prepared as T-SL and T-WP. T-SL is an aqueous solution and more environmentally friendly than other formulations (Chou et al, 2022 ). T-WP is a solid formulation suspended in water.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo antimicrobial activity of the fungal metabolite toluquinol against phytopathogenic bacteria

Chung,

Nguyen,

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionBacterial plant diseases cause tremendous economic losses worldwide. However, a few effective and sustainable control methods are currently available. To discover novel and effective management approaches, we screened marine fungi for their antibacterial activity against phytopathogenic bacteria in vitro and in vivo.MethodsWe screened the culture broth of 55 fungal strains isolated from various marine sources (seawater, algae, and sediment) for their in vitro antibacterial activity using the broth microdilution method. Then, only the fungal strain (designated UL-Ce9) with higher antibacterial activity in vitro was tested in an in vivo experiment against tomato bacterial wilt. The active compounds of UL-Ce9 were extracted using ethyl acetate, purified by a series of chromatography, and the structure was elucidated by nuclear magnetic resonance spectroscopy. Pesticide formulations of toluquinol were prepared as soluble concentrates and wettable powder. The disease control efficacy of toluquinol formulations was evaluated against blight of rice and the bacterial wilt of tomato.Results and discussionThe culture broth of UL-Ce9 showed high antibacterial activity against Agrobacterium tumefaciens, Ralstonia solanacearum, and Xanthomonas arboricola pv. pruni in vitro, and we selected UL-Ce9 for the in vivo test. The UL-Ce9 culture broth completely suppressed the bacterial wilt of tomato at a dilution of 1:5. The phylogenetic analysis identified UL-Ce9 as Penicillium griseofulvum, and the antibacterial metabolites were revealed as patulin, gentisyl alcohol, and toluquinol, all of which were associated with the biosynthetic pathway of the mycotoxin patulin. Patulin exhibited the highest antibacterial activity against 16 phytopathogenic bacteria in vitro, followed by toluquinol and gentisyl alcohol. As patulin is toxic, we selected toluquinol to investigate its potential use as a pesticide against bacterial plant diseases. Compared with the chemicals currently being applied in agriculture (streptomycin and oxytetracycline), toluquinol formulations exhibited similar and higher control efficacies against bacterial leaf blight of rice and bacterial wilt of tomato, respectively. To the best of our knowledge, this is the first report of the antibacterial activity of toluquinol against phytopathogenic bacteria. Our results suggest that toluquinol is a potential candidate for the development of novel and effective pesticides for the management of bacterial plant diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo antimicrobial activity of the fungal metabolite toluquinol against phytopathogenic bacteria

Chung,

Nguyen,

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Bacterial wilt of tomato, caused by Ralstonia pseudosolanacearum and R. solanacearum [155], are the major constrain in tomato losses in temperate, tropical and subtropical regions in the worldwide [23]. These pathogens have the ability to infect around 200 plant species, including those in the Solanaceae family [24]. The typical symptoms exhibited an infected tomato plants may include stunted growth, yellowing of leaves, vascular discoloration, wilting and ultimate cause death, all of which are associated with the presence of the bacterial wilt pathogens R. solanacearum and R. pseudosolanacearum [25].…”

Section: Bacterial Wiltmentioning

confidence: 99%

“…Chemical fungicides are commonly utilized to prevent various pests and diseases, but they have limited effectiveness and pose harm to crop physiology, human health and also harmful impact on environment [1]. In order to manage these diseases and decrease pathogen populations in crops, novel and alternative approaches like the utilization of fungi and bacteria as biocontrol agents have been implemented [24]. The possession of both antagonistic and plant growth-promoting characteristics by biocontrol agents is considered significant for managing plant diseases and improving fruit yield.…”

Section: Control Of Fungal and Bacterial Disease Through Bacillus Sp ...mentioning

confidence: 99%

Management of Phytopathogens by Antagonistic Bacillus spp in Tomato Crop

Iqbal,

Li,

Ahmed Rajput

et al. 2024

Tomato Cultivation and Consumption - Innovation and Sustainability

View full text Add to dashboard Cite

Bacillus is a genus of gram-positive bacteria that is widely distributed in the environment. The species of this genus present in the endosphere, phyllosphere and rhizosphere in the plant and perform as a beneficial biocontrol agent and promote plant health. These strains exhibit diverse capabilities, including the potentiality to directly suppress the germination of microbial, stimulate plant development, reduce pathogen infections, degrade different types of hydrocarbons, function effectively across a wide temperature range, and induce immune resistance in host plants. The species/strains of Bacillus genus have proven promising biocontrol agents against a large number of fungal and bacterial causal organism, as well as plant-damaging insects. They induce a wide range of composites with antifungal properties, such as iturin, surfactin, cyclic lipopeptides, bacillomycin, bacteriocins, polyketide, lentibiotics, phospholipid, polyketide microlectine, isocosmarin and amino sugar. These compounds play a crucial role in preventing and controlling diseases in plants. The synthesis of these compounds is initiated in response to the presence of bacterial and fungal pathogen biomass and their cell walls. The purpose of this review is to offer a thorough exploration of the disease suppression mechanisms utilized by Bacillus, with a specific emphasis on their function as plant growth-promoting rhizobacteria (PGPR).

show abstract

“…A medium modified by Chou [51] was prepared in a 10 L fermentation tank (model FS-V-D10P, Major Science Co., Ltd., Taoyuan, Taiwan). The medium, comprised of 0.75% molasses, 0.5% soy protein, 0.5% yeast powder, 0.1% K 2 HPO 4 , 0.1% KH 2 PO 4 , and 7 L of distilled water.…”

Section: Preparation Of Bacillus Velezensis Khh13 Rermentation Brothmentioning

confidence: 99%

Characterization of Biofertilization and Biocontrol Potential of Bacillus velezensis KHH13 from Organic Soils

Chen,

Tzean,

Chang

et al. 2024

Agronomy

View full text Add to dashboard Cite

Efficient and sustainable food production is crucial in global agricultural development. Overuse of chemical fertilizers leads to soil acidification, destruction of soil properties, and harm to soil micro-organisms. Plant growth-promoting rhizobacteria (PGPR) have emerged as a solution, enhancing soil fertility and crop yields while reducing chemical fertilizer dependency and disease occurrence. In this study, Bacillus strains KHC2, KHH5, and KHH13, isolated from organic rice field soils in Taiwan, were identified through molecular techniques as B. velezensis (KHC2, KHH13) and B. amyloliquefaciens (KHH5). The strains exhibited various hydrolytic enzymes (including protease, cellulase, amylase, and lecithinase), with KHH13 showing the highest phosphate solubilization (2186.1 µg mL−1 day−1) and indole-3-acetic acid (IAA) production (63.067 ± 0.595 ppm mL−1). These properties indicate KHH13’s potential as a bio-enhancer for plant growth. Therefore, we hypothesized that KHH13 can enhance plant growth and control soil-borne diseases. A greenhouse experiment demonstrated that KHH13, KHC2, and KHH5 effectively promoted the growth of red lettuce, with KHH13 showing superior efficacy. The study also found KHH13’s treatment enhanced the growth of various vegetables, including tomato, cucumber, and red lettuce. In terms of disease control, KHH13 significantly reduced Fusarium wilt in cucumbers, as evidenced by the reduction in disease index from 74.33% to 41.67% after KHH13 treatment. The treatment group displayed better plant growth, including plant height and fresh weight, compared to the control group in the greenhouse experiment. Furthermore, oral and pulmonary acute toxicity analysis in rats showed no adverse effects on rat weight or mortality, indicating KHH13’s safety for mammalian use. These findings suggest B. velezensis KHH13 as a safe, effective, and sustainable biological agent for enhancing vegetable growth and controlling soil-borne diseases, with potential applications in sustainable agriculture.

show abstract

Selection, Formulation, and Field Evaluation of Bacillus amyloliquefaciens PMB01 for Its Application to Manage Tomato Bacterial Wilt Disease

Cited by 7 publications

References 53 publications

In vitro and in vivo antimicrobial activity of the fungal metabolite toluquinol against phytopathogenic bacteria

In vitro and in vivo antimicrobial activity of the fungal metabolite toluquinol against phytopathogenic bacteria

Management of Phytopathogens by Antagonistic Bacillus spp in Tomato Crop

Characterization of Biofertilization and Biocontrol Potential of Bacillus velezensis KHH13 from Organic Soils

Contact Info

Product

Resources

About