2015
DOI: 10.1093/nar/gkv1057
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Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity

Abstract: Using a Systematic Evolution of Ligands by Exponential Enrichment (SELEX) protocol capable of selecting xeno-nucleic acid (XNA) aptamers, a 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamer (referred to as FA1) to HIV-1 reverse transcriptase (HIV-1 RT) was selected. FA1 bound HIV-1 RT with KD,app values in the low pM range under different ionic conditions. Comparisons to published HIV-1 RT RNA and DNA aptamers indicated that FA1 bound at least as well as these aptamers. FA1 contained a 20 nucleotide 5′ DNA se… Show more

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Cited by 80 publications
(125 citation statements)
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“…reported a 2′-FANA aptamer to human immunodeficiency virus-1 (HIV-1) reverse transcriptase 51 . This 2′-FANA aptamer was isolated from a 2′-FANA modified DNA pool through SELEX using electrophoretic mobility shift assay 51 .…”
Section: Aptamer Selection Using Sugar-modified Nucleotidesmentioning
confidence: 99%
See 1 more Smart Citation
“…reported a 2′-FANA aptamer to human immunodeficiency virus-1 (HIV-1) reverse transcriptase 51 . This 2′-FANA aptamer was isolated from a 2′-FANA modified DNA pool through SELEX using electrophoretic mobility shift assay 51 .…”
Section: Aptamer Selection Using Sugar-modified Nucleotidesmentioning
confidence: 99%
“…reported a 2′-FANA aptamer to human immunodeficiency virus-1 (HIV-1) reverse transcriptase 51 . This 2′-FANA aptamer was isolated from a 2′-FANA modified DNA pool through SELEX using electrophoretic mobility shift assay 51 . The developed 2′-FANA modified DNA aptamer, FA 1 had a K d value of 4 pM and showed greater resistance to nucleases 51 …”
Section: Aptamer Selection Using Sugar-modified Nucleotidesmentioning
confidence: 99%
“…2'F, 2'OMe, and 2'NH 2 nucleotides have been incorporated into RNA aptamers with Y639F mutant T7 RNA polymerase [1]. Other chemically modified nucleotides such as 2'-deoxy-2'-fluoroarabinonucleotide (FANA) [11], 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA)[12], and C2'-O-methyl(C2'-OMe)/ C2'-Fluorine (C2'-F) [13] have been incorporated into aptamers with engineered polymerase. The 2'-O-carbamoyl uridine (U cm ) is successfully incorporated by a wild-type T7 RNA polymerase [14].…”
Section: Chemically Modified Aptamersmentioning
confidence: 99%
“…To increase serum stability, in-SELEX and post-SELEX have been applied to incorporate modified nucleotide into aptamers. So far, it is very encouraging that 2′F, 2'OMe, 2'NH 2 [1], and newly developed modified nucleotides [1114] have been successfully incorporated with the engineered polymerases in the in-SELEX. Currently available modified nucleotides are modified at the 2′-position of ribose.…”
Section: Expert Opinionmentioning
confidence: 99%
“…Kuwahara and co-workers used a mixture of mutant and/or wild type KOD DNA polymerases to “transcribe” and “reverse transcribe” mixed LNA/DNA oligonucleotides during selection of an anti-thrombin aptamer [15,16]. Holliger and co-workers have dramatically expanded the potential of these analogs by evolving a series of TgoT DNA polymerase mutants to better “transcribe” and “reverse transcribe” these modified nucleotides, and they have used them to select HNA aptamers targeting HIV trans-activating response RNA and hen egg lysozyme [17•], a FANA aptamer targeting HIV-1 RT [18], and catalytic nucleic acids containing ANA, FANA, HNA, and CeNA moieties [19]. In addition, we have evolved several mutants of the Stoffel fragment that better “transcribe” and “reverse transcribe” fully modified 2’-F and 2’-OMe oligonucleotides [11].…”
Section: Selex With Modified Rna and Dnamentioning
confidence: 99%