Selection of a fungal isolate for the control of the pink hibiscus mealybug <i>Maconellicoccus hirsutus</i>

Ibarra-Cortés, Karla H.; Guzmán‐Franco, Ariel W.; González‐Hernández, Héctor; González-Camacho, Juan Manuel; Baverstock, J.

doi:10.1002/ps.3452

Cited by 10 publications

(3 citation statements)

References 29 publications

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“…Conidial production is a critical step for developing an economical mycopesticide given the large number of conidia required in field pest management applications 44 . In this study, a useful by‐product soybean meal from the oil industry was utilized during solid‐state fermentation to produce a cost‐effective and ecofriendly mycopesticide.…”

Section: Discussionmentioning

confidence: 99%

Isolation, fermentation, and formulation of entomopathogenic fungi virulent against adults of Diaphorina citri

Awan

Meng

Xia

et al. 2021

Pest Management Science

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BACKGROUND Mycopesticides are important for integrated management of the Asian citrus psyllid, Diaphorina citri. However, there are few reports on the fermentation and formulation for mycopesticides with high virulence against D. citri. RESULTS From four different locations in South China, 12 fungal strains were isolated and classified into Beauveria bassiana (two isolates), Fusarium fujikuroi (five isolates), and Cordyceps javanica (five isolates) based on the phylogenetic analysis of ITS1‐5.8S rDNA‐ITS4 and morphology of colonies and conidia. Based on the highest initial virulence (86.52 ± 2.35%) and best growth characteristics, F‐HY002‐ACPHali was further optimized for biphasic fermentation (7.85 ± 2.62 × 109 g–1 dry substrate) with soybean meal + cottonseed flour (1:1) as the solid substrate and full light as the light/dark cycle. Furthermore, the oil‐based conidial formulation was optimized with sesame oil as an effective carrier, which significantly enhanced conidial shelf life up to 16 weeks at 26 °C and 4 °C, thermo‐stress (50 °C) and UV‐B stress resistance, and virulence against adults of D. citri with increased mortalities and decreased LT50 in comparison to that of unformulated conidia. CONCLUSION Our results not only provide a valuable native strain with high virulence against adults of D. citri, but also imply the soybean meal + cottonseed flour as valuable solid substrate, full‐light exposure as environmental stimuli for solid‐state fermentation, and the sesame oil as an effective carrier for the formulation of the C. javanica. These findings will facilitate the development of a potential mycopesticide for the biological control of Asian citrus psyllid.

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Section: Discussionmentioning

confidence: 99%

Isolation, fermentation, and formulation of entomopathogenic fungi virulent against adults of Diaphorina citri

Awan

Meng

Xia

et al. 2021

Pest Management Science

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“…It is generally recognized that two key characteristics must be present or improved when choosing acceptable fungal BCA for use as biocides: (i) high virulence against the intended pests and (ii) environmental stress tolerance [ 15 , 16 ]. Despite the fact that these BCA have been studied for their potential to parasitize plant pathogens [ 5 , 6 ], little research focus has been placed on determining how well these isolates can withstand abiotic stress and/or recover from it.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Assessment of Eight Selected Indigenous Fungal Isolates Tolerance to Various Abiotic Stresses and their Effects on Seed Germination

Ramatsitsi,

Khosa,

Mashamaite

et al. 2023

Curr Microbiol

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Fungal bio-control agents (BCA) can minimize use of agro-chemicals while increasing plant productivity and tolerance to biotic-abiotic stressors. Ideally, BCA should tolerate varying environmental conditions they are introduced into, to successfully dominate and protect plants from stressors. However, BCA are living micro-organisms, their survival and efficacy can be impeded by extreme conditions. The current study aimed at evaluating whether indigenous fungal isolates, viz, Aspergillus flavus, A. terreus, Penicillium sp. AL-38 IRH-2012b, Talaromyces minioluteus, T. purpureogenus, T. sayulitensis, Trichoderma ghanense and T. viride can tolerate different levels of salinity, pH, nutrient and temperature. Certain fungal species are pests with potential of destroying many crops; the pathogenic effects of the aforementioned fungal isolates were further assessed on different crops’ seeds. The results showed that, although being indigenous, Aspergillus, T. sayulitensis and T. ghanense failed to thrive in high salinity and pH. While Penicillium sp. AL-38 IRH-2012b failed to thrive under reduced nutrient level and all fungal isolates failed to grow at 10–20 °C. Furthermore, it was noted species within the same genus could affect crops in both favorable and unfavorable ways. The study demonstrated that the selected indigenous fungal isolates can tolerate different abiotic conditions and have potential to improve seed germination and seedling growth.

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“…Temperature affects the relationship between EPF and the host thus determining the success of plant pest control to a large extent [10][11][12], but thermal requirements often vary even within one species [13,14]. Research on the thermal requirements of EPF is mainly limited to determining the minimum and maximum temperature at which the pathogen is able to function [15,16]. For example, most isolates of EPF of the Hypocreales (Ascomycota) order grow in the temperature range from 8 • C to 30-32 • C, with their optima varying from 20 • C to 30 • C. In turn, the temperature that limits their development is 5 • C and 35 • C [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Temperature Requirements for the Colony Growth and Conidial Germination of Selected Isolates of Entomopathogenic Fungi of the Cordyceps and Paecilomyces Genera

Tkaczuk,

Majchrowska-Safaryan

2023

Agriculture

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The aim of the study was to determine the effect of temperature on the colony growth and conidia germination of selected species of entomopathogenic fungi in the genus Cordyceps (C. farinosa, C. fumosorosea and C. coleopterorum) and one isolate of Paecilomyces suffultus. In the first part of the experiment, selected isolates were grown on Sabouraud (SDA) medium at six temperatures: 5, 10, 15, 20, 25 and 30 °C. Colony growth was observed every 3 days until day 18, by measuring the colony diameter. In the second part of the experiment, slides with an SDA medium and spores were placed in incubators with the above temperature and observations on conidia germination were carried out after 24 and 48 h. The results revealed that the thermal optimum for colony growth of the fungal isolates was within the temperature range of 15 °C and 25 °C. The optimum temperature for the growth of P. suffultus colonies was 15 °C, with 20 °C for C. farinosa and C. coleopterorum. The highest thermal requirements were demonstrated by the C. fumosorosea, which developed best at 25 °C. Cordyceps farinosa and C. fumosorosea developed in a wider temperature range, from 5 °C to 30 °C. In contrast, growth of C. coleopterorum and P. suffultus colonies was observed only at temperatures between 10 °C and 25 °C. After 24 h, spore germination of the fungal species was most intense at 25 °C. After both 24 and 48 h, the temperature of 5 °C stopped the spore germination of all fungal species, and in the case of C. farinosa and C. fumosorosea no germination was also found at 30 °C. This study on the effect of temperature on the growth and spore germination of the species C. coleopterorum and P. suffultus is the first research of its type. The fungal isolates tested in this work in terms of thermal requirements have shown high pathogenicity in relation to selected plant pests in previous studies, which indicates their potential usefulness in IPM programs.

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Selection of a fungal isolate for the control of the pink hibiscus mealybug Maconellicoccus hirsutus

Abstract: Isolate Ma65 shows the best potential to be developed as a microbial control agent for M. hirsutus.

Cited by 10 publications

References 29 publications

Isolation, fermentation, and formulation of entomopathogenic fungi virulent against adults of Diaphorina citri

Isolation, fermentation, and formulation of entomopathogenic fungi virulent against adults of Diaphorina citri

In Vitro Assessment of Eight Selected Indigenous Fungal Isolates Tolerance to Various Abiotic Stresses and their Effects on Seed Germination

Temperature Requirements for the Colony Growth and Conidial Germination of Selected Isolates of Entomopathogenic Fungi of the Cordyceps and Paecilomyces Genera

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