Selection of a nucleopolyhedrovirus isolate from<i>Helicoverpa armigera</i>as the basis for a biological insecticide

Arrizubieta, Maite; Williams, Trevor; Caballero, Primitivo; Simón, Oihane

doi:10.1002/ps.3637

Cited by 29 publications

(38 citation statements)

References 42 publications

(89 reference statements)

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“…Moreover, additional genotypes may be present in the HearSP1-wt population, thus attenuating the influence of HearSP1B on HearSP1-wt transmissibility, as occurs with certain genotypic variants in wild-type isolates of SeMNPV (18) and Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) (16). In contrast, among the different variants isolated from larvae that died during laboratory rearing, no marked differences in pathogenicity were detected, but differences in speed of kill were observed, with HearLB6 being among the fastest-killing variants, even more so than HearSP1-wt, which was previously selected as one of the fastest-killing isolates from diseased larvae collected in the Iberian Peninsula (36). These results are in line with data from previous studies performed with different variants of HearSNPV, in which variants of similar pathogenicities presented marked differences in speed of kill (36,50).…”

Section: Discussionmentioning

confidence: 99%

“…The HearSNPV-SP1 (HearSP1) isolate was originally obtained from diseased larvae from tomato crops in Guadajira, Badajoz, Spain. HearSP1 was included as a reference isolate in this study because it was previously identified as a highly pathogenic isolate in terms of concentration-mortality metrics (27,36). From a total of 17 HearSNPV isolates obtained from individual larvae that died of polyhedrosis disease during rearing of H. armigera larvae collected from cotton crops in Lebrija, Spain, six different EcoRI restriction endonuclease profiles were identified.…”

Section: Methodsmentioning

confidence: 99%

“…OBs were extracted from virus-killed larvae as described previously (36). Briefly, individual cadavers were homogenized in 300 l water, filtered through a piece of muslin, washed with 500 l of 0.1% sodium dodecyl sulfate (SDS), and then washed twice in distilled water.…”

Section: Isolation Of Hearsnpv Genotypic Variants (I)mentioning

confidence: 99%

“…Concentration-mortality responses were determined by using five OB concentrations of each variant that were estimated to result in a range of mortality rates of between ϳ5 and ϳ95% (36). Groups of 24 secondinstar insects that had molted in the previous 12 h were inoculated with each OB concentration in each replicate; larvae that consumed the inoculum within 10 min were individually transferred into 25-ml cups with diet and reared at 25°C Ϯ 1°C in darkness.…”

Section: Isolation Of Hearsnpv Genotypic Variants (I)mentioning

confidence: 99%

“…A Spanish field isolate of HearSNPV obtained from viruskilled larvae collected at Guadajira, Badajoz, Spain, named Hear-SNPV-SP1 (HearSP1), showed a suitable insecticidal phenotype, in terms of occlusion body (OB) pathogenicity, speed of kill, and OB productivity, compared with isolates from other parts of the Iberian Peninsula (36). Some of the genotypes present in the HearSP1 isolate were identified as the active ingredient for a potential biological insecticide (40).…”

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confidence: 99%

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A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

Arrizubieta

Simón

Williams

et al. 2015

Appl Environ Microbiol

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The genotypic diversity of two Spanish isolates of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) was evaluated with the aim of identifying mixtures of genotypes with improved insecticidal characteristics for control of the cotton bollworm. Two genotypic variants, HearSP1A and HearSP1B, were cloned in vitro from the most pathogenic wild-type isolate of the Iberian Peninsula, HearSNPV-SP1 (HearSP1-wt). Similarly, six genotypic variants (HearLB1 to -6) were obtained by endpoint dilution from larvae collected from cotton crops in southern Spain that died from virus disease during laboratory rearing. Variants differed significantly in their insecticidal properties, pathogenicity, speed of kill, and occlusion body (OB) production (OBs/larva). HearSP1B was ϳ3-fold more pathogenic than HearSP1-wt and the other variants. HearLB1, HearLB2, HeaLB5, and HearLB6 were the fastest-killing variants. Moreover, although highly virulent, HearLB1, HearLB4, and HearLB5 produced more OBs/ larva than did the other variants. The co-occluded HearSP1B:LB6 mixture at a 1:1 proportion was 1.7-to 2.8-fold more pathogenic than any single variant and other mixtures tested and also killed larvae as fast as the most virulent genotypes. Serial passage resulted in modified proportions of the component variants of the HearSP1B:LB6 co-occluded mixture, suggesting that transmissibility could be further improved by this process. We conclude that the improved insecticidal phenotype of the HearSP1B:LB6 co-occluded mixture underlines the utility of the genotypic variant dissection and reassociation approach for the development of effective virus-based insecticides.A lphabaculoviruses (Baculoviridae) are lepidopteran-specific nucleopolyhedroviruses (NPVs) that have been used successfully as biological control agents against several agricultural and forest pests (1). These viruses are characterized by high intraspecific heterogeneity not only between isolates from different geographic regions (2, 3) but also within single isolates that often comprise mixtures of several genotypes present in different proportions (4-8). The molecular heterogeneity of genotypes is often associated with phenotypic differences in pathogenicity, speed of kill, and virus production (8, 9), which are traits of practical importance for the use of these viruses as biological insecticides (10). Infections involving mixtures of genotypes can result in positive (11-14) or negative (15-18) effects on a number of these traits.As natural alphabaculovirus populations in virus-killed insects comprise mixtures of genotypes, the genotypic interactions that modulate the insecticidal characteristics have attracted the attention of insect pathologists (8,(19)(20)(21) and have been used to develop novel products based on unique user-defined genotypic combinations in specific user-defined proportions that result in improved insecticidal properties of the final mixture (22,23). This has resulted in the generation of a novel paradigm for the development of baculovirus-based insectici...

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Isolation Of Hearsnpv Genotypic Variants (I)mentioning

confidence: 99%

Section: Isolation Of Hearsnpv Genotypic Variants (I)mentioning

confidence: 99%

mentioning

confidence: 99%

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A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

Arrizubieta

Simón

Williams

et al. 2015

Appl Environ Microbiol

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Insecticidal efficacy and persistence of a co‐occluded binary mixture of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) variants in protected and field‐grown tomato crops on the Iberian Peninsula

Arrizubieta

Simón

Torres‐Vila

et al. 2015

Pest Management Science

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BACKGROUND A binary co‐occluded mixture (HearSP1B:LB6) of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) variants was previously found to be highly pathogenic under laboratory conditions. The insecticidal efficacy and persistence of this mixture were determined in greenhouse and field‐grown tomato crops in Spain and Portugal. RESULTS Concentrations of 109–1011 occlusion bodies (OBs) L−1 of HearSP1B:LB6 resulted in 89–100% mortality of larvae on treated tomato plants in growth chambers. In protected tomato crops, application of 1010 OBs L−1 of HearSP1B:LB6 was as effective as Bacillus thuringiensis (Bt) and spinosad in reducing the percentage of damaged fruits, and resulted in higher larval mortality than the Bt treatment. In open‐field tomato crops, virus treatments were as effective in reducing the percentage of damaged fruit as spinosad, Bt and chlorpyrifos treatments. The persistence of the insecticides on tomato plants was negatively correlated with solar radiation in both field and greenhouse settings. Residual insecticidal activity of OBs on protected tomato crops at 6 days post‐application was 55 and 35% higher than that of Bt and spinosad respectively. On field‐grown tomato, OB persistence was significantly lower than with spinosad or chlorpyrifos. CONCLUSION The efficacy and persistence of HearSP1B:LB6 OBs were comparable with those of commercial insecticides in both field and greenhouse tomato crops. Future studies should focus on reducing application rates to determine insecticidal efficacy at lower OB concentrations. © 2015 Society of Chemical Industry

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Leveraging insect viruses and genetic manipulation for sustainable agricultural pest control

Sun,

Fu,

et al. 2023

Pest Management Science

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Insect viruses have long been recognized for their potential as biological control agents in managing agricultural pests. Despite their promise, challenges such as host specificity, virulence, and limited viral resources have hindered their widespread application. The need for efficient discovery and utilization of insect viruses is paramount for sustainable agricultural practices. With the introduction of high‐throughput sequencing (HTS) technologies, the discovery and identification of new insect viruses have been significantly accelerated, enriching the available resources for pest management. Furthermore, advancements in reverse genetics tools have enabled the development of multifunctional viral expression vectors, enhancing the specificity and efficacy of insect viruses against targeted pests. This review provides a comprehensive overview of the methodologies for insect virus identification using HTS and the potential of genetically modified insect viruses in pest control. Genetically engineered insect viruses offer a promising avenue for targeted and efficient pest management. While HTS technologies have revolutionized the discovery of insect viruses, challenges remain. Addressing these challenges and harnessing the potential of these technologies will be pivotal in shaping sustainable agricultural management strategies in the future.This article is protected by copyright. All rights reserved.

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Selection of a nucleopolyhedrovirus isolate fromHelicoverpa armigeraas the basis for a biological insecticide

Abstract: The insecticidal properties of HearSNPV-SP1 mean that this strain is likely to prove useful as the basis for a biological insecticide for control of Helicoverpa armigera in Spain.

Cited by 29 publications

References 42 publications

A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

Insecticidal efficacy and persistence of a co‐occluded binary mixture of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) variants in protected and field‐grown tomato crops on the Iberian Peninsula

Leveraging insect viruses and genetic manipulation for sustainable agricultural pest control

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